Method and system for extracting ergosterol from zymophyte residues

A technology of ergosterol and fermentation bacteria, applied in the direction of steroids, organic chemistry, etc., can solve the problems of long extraction process and high energy consumption, achieve the effect of optimizing extraction process parameters, improving product quality, and reducing extraction process consumption

Pending Publication Date: 2020-12-29
内蒙古麦维生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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The patented extraction proces

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  • Method and system for extracting ergosterol from zymophyte residues
  • Method and system for extracting ergosterol from zymophyte residues
  • Method and system for extracting ergosterol from zymophyte residues

Examples

Experimental program
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Effect test

Embodiment 1

[0130] (1) Extraction: Weigh 1 kg of penicillin fermentation residue, add it to the extraction tank, add 1.2 kg of methanol, heat to maintain the temperature at 55 ° C, add 70 g of solid sodium hydroxide, stir for 6 hours, then add 1.2 kg of petroleum ether, and heat Maintain the temperature at 50°C and stir for 3 hours.

[0131] (2) Filtration and phase separation: the material after the extraction reaction in step (1) is vacuum filtered, the filtrate is collected, the filtrate is pumped into the phase separation tank and left to stratify, the upper layer light phase liquid is collected, and the lower layer solvent is recovered. At the same time, 0.5 kg of filter cake was collected.

[0132] (3) Decolorization and crystallization: Add the upper layer liquid collected in step (2) filtration and phase separation into a decolorization tank with a stirring device, maintain the temperature at 50°C, add 1g of activated carbon, stir for 2 hours, filter while hot, and collect the fil...

Embodiment 2

[0135] (1) Extraction: Weigh 1 kg of penicillin fermentation residue, add it to the extraction tank, add 1.2 kg of methanol, heat to maintain the temperature at 55 ° C, add 70 g of solid sodium hydroxide, stir for 6 hours, then add 1.2 kg of petroleum ether, and heat Maintain the temperature at 50°C and stir for 3 hours.

[0136] (2) Filtration and phase separation: the material after the extraction reaction in step (1) is vacuum filtered, the filtrate is collected, the filtrate is pumped into the phase separation tank and left to stratify, the upper layer light phase liquid is collected, and the lower layer solvent is recovered. At the same time, 0.5 kg of filter cake was collected.

[0137] (3) Second extraction of filter cake: add 0.6kg of petroleum ether to the extraction tank, then add 0.5kg of filter cake produced in step (2) after filtration and phase separation; heat up to 50°C, stir and maintain the temperature for extraction for 2 hours .

[0138] (4) Filtration an...

Embodiment 3

[0142] (1) Extraction: Weigh 1 kg of penicillin fermented bacteria residue, add it to the extraction tank, add 1.2 kg of chloroform, heat to maintain the temperature at 57° C., add 50 g of sodium hydroxide, and stir for 4 hours. Add 1.2 kg of n-heptane, heat to maintain the temperature at 50°C, and stir for 3 hours.

[0143](2) Filtration and phase separation: the material after the extraction reaction in step (1) is vacuum filtered, the filtrate is collected, the filtrate is pumped into the phase separation tank and left to stratify, the upper layer light phase liquid is collected, and the lower layer solvent is recovered.

[0144] (3) Decolorization and crystallization: Add the upper light phase liquid collected in step (2) filtration and phase separation into a decolorization tank with a stirring device, heat up to 60°C, add 1g of activated carbon, stir for 2 hours, and use vacuum pumping while it is hot Filter, collect the filtrate, heat and distill under negative pressure...

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Abstract

The invention discloses a method for extracting ergosterol from zymophyte residues, which comprises the following steps: S1, extraction: adding a solvent, a saponifying agent and an extracting agent into the zymophyte residues containing ergosterol, and carrying out saponification and extraction reaction to obtain an extraction mixture; S2, carrying out phase splitting, namely carrying out phase splitting on the extracted mixture in the step S1 to obtain an extracting solution containing ergosterol; S3, crystallization: concentrating and crystallizing the extracting solution in the step S2 toobtain an ergosterol crude product. The invention further discloses a system for extracting ergosterol from the zymophyte residues. The method has the advantages of short process flow, short extraction time, low energy consumption in the extraction process, high product purity and the like.

Description

technical field [0001] The invention belongs to the field of pharmaceutical intermediates, in particular to a method and system for extracting ergosterol from fermented bacteria residues. Background technique [0002] Ergosterol is also known as ergosterol. Ergosterol is an important component of microbial cell membranes, and plays an important role in ensuring the integrity of cell membranes, the activity of membrane-bound enzymes, membrane fluidity, cell viability, and cell material transport. Ergosterol is a precursor for the production of vitamin D2 and an intermediate in the production of hormone drugs, which can be used to produce cortisone. It has the effect of vitamin D2. [0003] The traditional synthetic method of ergosterol is mainly based on microbial fermentation supplemented by traditional chemical synthesis and separation. The production process starts from ERG9 to prepare ergosterol. The high cost leads to high product prices and insufficient market supply...

Claims

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Application Information

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IPC IPC(8): C07J9/00
CPCC07J9/00
Inventor 潘桀杨志刚畅通杨东泽
Owner 内蒙古麦维生物科技有限公司
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