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Composite material for improving microbial diversity and inhibiting pathogenic bacteria and preparation method thereof

A composite material and diverse technology, applied in the fields of botanical equipment and methods, organic fertilizers, inorganic fertilizers, etc., can solve the problems of high proportion of pathogenic bacteria and poor diversity of environmental microbial communities.

Active Publication Date: 2021-01-01
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of poor environmental microbial community diversity and high proportion of pathogenic bacteria in current garden materials, and provide a composite material and its preparation method that can improve microbial diversity and inhibit pathogenic bacteria

Method used

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  • Composite material for improving microbial diversity and inhibiting pathogenic bacteria and preparation method thereof
  • Composite material for improving microbial diversity and inhibiting pathogenic bacteria and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The used raw material component of inoculant in the embodiment is specifically:

[0027] 1. Horticultural peat soil, the peat soil is a common landscaping soil, generally through composting treatment technology (60-70 degrees Celsius in the high temperature stage, at least one hour), does not contain plant seeds.

[0028] 2. Mixed soil of pine needles and leaves on the surface layer of coniferous and broad-leaved mixed forest. This type of soil is taken from the mixed coniferous and broad-leaved forest. The main tree species include: Pinus sylvestris, larch, white birch, walnut catalpa, Mongolian oak, etc., including all litter layers and 10cm surface layer soil.

[0029] 3. Activated carbon: adopt GB / T 7701.7-1997 coal-based granular activated carbon for high-efficiency adsorption, particle pore size: 2mm.

[0030] 4. Dried moss: dry water moss for gardens.

[0031] 5, fresh pine needles: can be the needles of any kind (embodiment adopts Pinus sylvestris needles).

...

Embodiment 2-4 and comparative example 1-3

[0043] Embodiment 2-4 is the preparation of composite material, the inoculation agent of embodiment 2-4 uses the inoculation agent prepared in embodiment 1, and specific formula sees table 2 for details, and specific preparation method is: add cyclodextrin and supernatant to inoculant Calcium oxide, the humidity is adjusted to 50%, the pH is adjusted to 7 ± 0.5, and composting is carried out for 2 weeks (25 ± 5 degrees Celsius), and it is protected from rain and fully mixed.

[0044] Table 2 embodiment 2-4 and comparative example 1-3 are composite material raw material proportioning (weight part)

[0045] components Example 2 Example 3 Example 4 Comparative example 1 Comparative example 2 Comparative example 3 Inoculants 100 100 100 100 100 100 Cyclodextrin 1 0.5 1.5 - 1 - calcium peroxide 2 1.0 3.0 2 - -

Embodiment 5

[0047] The specific application effects of the composite materials obtained in the following test examples 2-4 and comparative examples 1-3 are as follows: 25 parts of composite materials are added to 75 parts of landscaping soil, and then the two are fully mixed and applied In the outdoor environment, the species abundance diversity index and the number of pathogenic bacteria were detected, and the results are shown in Table 3.

[0048] Among them, the species abundance of the microbial community was detected using the Illumina Miseq high-throughput sequencing platform to analyze the bacterial 16s rRNA gene. The total number of bacterial gene sequences was 15,000, and the human infectious pathogen index (sum) was calculated by Picrust software.

[0049] Table 3 embodiment 8-14 is application effect test result

[0050]

[0051] Note: The species abundance and diversity index refers to the original garden soil without adding composite materials.

[0052]It can be seen from...

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Abstract

The invention relates to a composite material for improving microbial diversity and inhibiting pathogenic bacteria and a preparation method of the composite material. The composite material comprisesthe following raw material components in parts by weight of 90-110 parts of inoculant, 0.5-1.5 parts of cyclodextrin and 1-3 parts of calcium peroxide. The inoculant comprises the following raw material components in percentage by weight of 45-55% of peat soil, 15-25% of mixed broadleaf-conifer forest soil, 5-15% of activated carbon, 1-3% of dry moss, 0.3-0.8% of fresh pine needles, 1-3% of freshbroad leaves, 3-8% of fresh shrub branches, 0.5-1.5% of shrub fresh leaves, 3-8% of dry pine bark, 0.3-0.8% of fresh usnea and 3-5% of dry corn stalks. The composite material can be applied to landscaping materials (soil, gravel and turf), pathogenic bacteria in the landscaping materials are inhibited, the diversity of microbial communities in the landscaping materials is improved, and the purposeof optimizing the microbial communities around a human body is achieved.

Description

technical field [0001] The invention relates to the field of garden materials, in particular to a composite material for improving microbial diversity and inhibiting pathogenic bacteria and a preparation method thereof. Background technique [0002] The current global urbanization process is rapid, the population is becoming denser and denser, and the pressure on the urban ecological environment is intensifying. In densely populated cities, the pollution is intensified and the ecology is damaged, followed by the outbreak of immune-mediated diseases and infectious diseases. Common ones include eczema, allergic dermatitis, bronchitis, pneumonia, tuberculosis, etc. The pressure on public health and disease prevention and control is huge, and the medical system is overwhelmed. How to reduce the "overboard" by improving the ecological environment in the upper reaches of the river is the demand of the disease prevention and control department. [0003] Environmental microbes are...

Claims

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Application Information

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IPC IPC(8): A01G18/20C05G3/00C05G3/60
CPCA01G18/20C05D3/00C05G3/00C05G3/60C05D9/00C05F11/00C05F11/02
Inventor 惠楠刘欣欣
Owner SHANGHAI JIAO TONG UNIV
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