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Norovirus vaccine based on chimpanzee adenovirus vector and preparation method and application thereof

A virus vaccine and adenovirus technology, applied in the field of vaccines, can solve the problems of difficulty in the development of norovirus vaccines and no norovirus vaccines on the market.

Active Publication Date: 2021-01-01
IMMUNE PATH BIOTECHNOLOGY SUZHOU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the lack of small animal models and virus in vitro cell culture systems, the development of norovirus vaccines is difficult, and no norovirus vaccines are currently on the market

Method used

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  • Norovirus vaccine based on chimpanzee adenovirus vector and preparation method and application thereof
  • Norovirus vaccine based on chimpanzee adenovirus vector and preparation method and application thereof
  • Norovirus vaccine based on chimpanzee adenovirus vector and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] 1. Experimental materials

[0062] 1.1 cells

[0063] The HEK293 cell line was purchased from ATCC cell bank in the United States (product number: CRL-1573), and the medium was DMEM (GIBCO, #11995-040) containing 10% fetal bovine serum (GIBCO, #10091-148).

[0064] 1.2 BALB / c mice

[0065] All the mice involved in this experiment were purchased from Shanghai Xipuer-Bikay Experimental Animal Co., Ltd. All animal experiments were carried out in the SPF barrier of Shanghai Experimental Animal Resource Center.

[0066] 2. Experimental method

[0067] 2.1 Packaging of recombinant chimpanzee adenovirus ChAd63-GII.4

[0068] Plasmid pChAd63-GII.4 was synthesized by Suzhou Jinweizhi Biotechnology Co., Ltd. restriction endonuclease SwaI linearized the plasmid pChAd63-GII.4. Cover 6-well plates with HEK293 cells, and when the cell density reaches 80%–90%, transfect about 6 μg of the linearized pChAd63-GII.4 digest product with the transfection reagent Lipofectamine 2000 (...

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Abstract

The invention discloses a norovirus vaccine based on a chimpanzee adenovirus vector and a preparation method and application thereof. The norovirus vaccine is prepared by the following steps that a nucleotide sequence of a norovirus target protein is inserted into a recombinant chimpanzee adenovirus vector plasmid to construct a recombinant plasmid, and then packaging and processing are carried out to obtain the norovirus recombinant adenovirus vaccine with immunogenicity. According to the norovirus vaccine, the norovirus vaccine is developed for the first time based on the chimpanzee adenovirus vector, and the vaccine cannot be influenced by a pre-stored adenovirus antibody in a human body; the adenovirus vector vaccine is simple to prepare and good in safety, an adjuvant does not need tobe added, and immunization routes such as muscle immunization or oral immunization can be adopted; and the norovirus preventive vaccine is prepared on the basis of the replication-defective chimpanzee adenovirus vector, and a vaccine product which is easy to prepare, low in cost, good in safety, free of adjuvant and wide in application prospect is expected to be obtained.

Description

technical field [0001] The invention relates to the technical field of vaccines, in particular to a norovirus vaccine based on a chimpanzee adenovirus vector, a preparation method and application thereof. Background technique [0002] Norovirus, also known as Norwalk Viruses, is a highly contagious virus in the Human Calicivirus family (Human Calicivirus) that is mainly transmitted through the fecal-oral route. Major causative agent of intermittent outbreaks of nonbacterial gastroenteritis in China. Norovirus has no obvious pathogenicity in healthy people, but it can cause severe disease and last for a long time in immunocompromised patients, the elderly and children. Norovirus diarrhea is prevalent worldwide. In developed countries such as the United States, the Netherlands, the United Kingdom, Japan, and Australia, 60–90% of the annual outbreaks of abacterial diarrhea are caused by noroviruses. Among Chinese children with diarrhea under 5 years old, the detection rate o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61P31/14C12N15/861C07K14/08
CPCA61K39/12A61K2039/5256A61P31/14C07K14/005C12N15/86C12N2710/10043C12N2770/16022C12N2770/16034
Inventor 史力侯永凡张超张玲莉刘海涛李军瑞王紫琰朱钱钧周晨亮田文莉刘革
Owner IMMUNE PATH BIOTECHNOLOGY SUZHOU CO LTD
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