Norovirus vaccine based on chimpanzee adenovirus vector and preparation method and application thereof

Abstract

The invention discloses a norovirus vaccine based on a chimpanzee adenovirus vector and a preparation method and application thereof. The norovirus vaccine is prepared by the following steps that a nucleotide sequence of a norovirus target protein is inserted into a recombinant chimpanzee adenovirus vector plasmid to construct a recombinant plasmid, and then packaging and processing are carried out to obtain the norovirus recombinant adenovirus vaccine with immunogenicity. According to the norovirus vaccine, the norovirus vaccine is developed for the first time based on the chimpanzee adenovirus vector, and the vaccine cannot be influenced by a pre-stored adenovirus antibody in a human body; the adenovirus vector vaccine is simple to prepare and good in safety, an adjuvant does not need tobe added, and immunization routes such as muscle immunization or oral immunization can be adopted; and the norovirus preventive vaccine is prepared on the basis of the replication-defective chimpanzee adenovirus vector, and a vaccine product which is easy to prepare, low in cost, good in safety, free of adjuvant and wide in application prospect is expected to be obtained.

Description

technical field

[0001] The invention relates to the technical field of vaccines, in particular to a norovirus vaccine based on a chimpanzee adenovirus vector, a preparation method and application thereof. Background technique

[0002] Norovirus, also known as Norwalk Viruses, is a highly contagious virus in the Human Calicivirus family (Human Calicivirus) that is mainly transmitted through the fecal-oral route. Major causative agent of intermittent outbreaks of nonbacterial gastroenteritis in China. Norovirus has no obvious pathogenicity in healthy people, but it can cause severe disease and last for a long time in immunocompromised patients, the elderly and children. Norovirus diarrhea is prevalent worldwide. In developed countries such as the United States, the Netherlands, the United Kingdom, Japan, and Australia, 60–90% of the annual outbreaks of abacterial diarrhea are caused by noroviruses. Among Chinese children with diarrhea under 5 years old, the detection rate o...

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