Preparation method and application of complex microbial agent for removing nitrogen-containing pollutants in high-salinity wastewater

A technology of compound bacterial agent and high-salt wastewater, applied in the field of microorganisms, can solve the problems of poor impact load resistance, unstable treatment effect, high operating cost, etc., achieve low cost, overcome toxic effects, and solve the effects of biological nitrogen removal limitations

Active Publication Date: 2021-01-05
CHONGQING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the need to add a large amount of chemicals in the physical and chemical methods, the treatment cost is high, and the excessive amount of chemicals is easy to cause secondary pollution, so it has the disadvantages of high operating costs and polluting the environment; biological methods are widely used in wastewater treatment because of their economy and high efficiency. Denitrification treatment
[0004] Traditional biological methods have great advantages in denitrification treatment under low salinity conditions, but when the salinity exceeds 3.5%, due to the high concentration of chloride ions in high-salt wastewater, it will quickly change the cell osmotic pressure of microorganisms and destroy Bacterial cells can inhibit the growth of bacteria, especially for NOB (Nitrite-oxidizing bacteria) bacteria and anaerobic ammonia oxidation bacteria. Therefore, for high-salt wastewater, commonly used biological methods include activated sludge method, Oxidation pond method and constructed wetland method, etc. Among these methods: activated sludge method has poor impact load resistance; oxidation pond method and constructed wetland method occupy a large area, and the treatment effect is unstable due to natural factors such as season and temperature. and the effect is not obvious

Method used

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  • Preparation method and application of complex microbial agent for removing nitrogen-containing pollutants in high-salinity wastewater
  • Preparation method and application of complex microbial agent for removing nitrogen-containing pollutants in high-salinity wastewater
  • Preparation method and application of complex microbial agent for removing nitrogen-containing pollutants in high-salinity wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Step 1) Acclimatization of strains

[0059] Acclimatization of Rhodococcus crimson C1 (CCTCC NO: M 2019725) with high salt tolerance: take 2 mL of bacterial liquid and inoculate 250 mL of triangular medium containing 100 mL of sterilized CM medium (nitrate nitrogen concentration: 490mg / L) in a bottle, shake well, 30°C, 150 r min -1 Conditioned culture 3d. Under the same conditions as above, increase the concentration of NaCl and carry out continuous subculture for a total of 6 passages. The corresponding NaCl concentrations are 3%, 5%, 7%, 10%, 13%, and 15%.

[0060] High-salt tolerance acclimatization of Zoonococcus lepitoides (CICC 23939): Inoculate 2 mL of bacterial liquid into a 250 mL Erlenmeyer flask containing 100 mL of sterilized CM medium (nitrate nitrogen concentration: 490 mg / L) , shake well, 30°C, 150 r min -1 Conditioned culture 3d. Increase the NaCl concentration (the same as increasing the NaCl concentration of Rhodococcus crimson C1, the same below),...

Embodiment 2

[0079] Example 2: Efficient nitrogen removal experiment of compound bacterial agent in artificial wastewater with a salinity of 12%

[0080] The artificial wastewater formula is: sodium acetate 4g, NaNO 3 3g / L, KH 2 PO 4 0.35g / L, NaHCO 3 0.56g / L, the nitrogen concentration in the obtained artificial wastewater is 490 mg / L.

[0081] Take 100 mL of artificial wastewater in a 250 mL Erlenmeyer flask, and add 2 mL of compound bacterial agent solution (OD 600 0.9 to 1.2), sealed with a parafilm, placed in a shaker set at 30°C, 150 r min -1 conditions, and then measure the OD of the bacterial solution every 24 h 600 value to determine the growth of bacteria, and at the same time measure the content of nitrogen (nitrate) in the artificial wastewater to determine the removal effect of nitrogen. Depend on figure 1 It can be seen that the removal rate of nitrogen (nitrate) in artificial wastewater by the compound bacterial agent is as high as 91.2% under the condition of salini...

Embodiment 3

[0082] Example 3: Efficient Nitrogen Removal Experiment of Compound Bacteria at Different Salinity

[0083] The artificial wastewater formula is: sodium acetate 4g, NaNO 3 3g / L, KH 2 PO 4 0.35g / L, NaHCO 3 0.56g / L, the nitrogen concentration in the obtained artificial wastewater is 490 mg / L.

[0084] Take 100 mL of artificial wastewater in a 250 mL Erlenmeyer flask, and add 2 mL of compound bacterial agent solution (OD 600 1-2), sealed with parafilm, set different conical flasks to different salinities (3%, 5%, 7%, 10%, 13%, 15%), put them into the shaker and set at 30°C, 150 rpm -1 Cultivate under conditions, measure the OD of the bacterial solution 600 value to determine the growth of bacteria, and at the same time measure the content of nitrogen (nitrate) in the artificial wastewater to determine the removal effect of nitrogen.

[0085] Depend on figure 2 It can be seen that the composite bacterial agent can efficiently remove nitrogen (nitrate) in artificial waste...

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Abstract

The invention relates to a preparation method and application of a complex microbial agent for removing nitrogen-containing pollutants in high-salinity wastewater. The complex microbial agent is prepared by compounding Rhodococcus ruber C1, Planococcus rifietoensis, pseudomonas aeruginosa, alcaligenes faecalis and acinetobacter. The complex microbial agent provided by the invention has the characteristics of high salt resistance and high nitrogen resistance, and can realize efficient denitrification of high-nitrogen wastewater in an aerobic environment under a high-salinity condition through the synergistic effect of five microorganisms.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to a preparation method and application of a composite bacterial agent for removing nitrogenous pollutants in high-salt wastewater. Background technique [0002] With the intensification of my country's industrialization process, the amount of high-salt (salinity > 3%) wastewater produced by chemical wastewater, pickling wastewater, printing and dyeing wastewater, and oil recovery wastewater has also increased significantly, and most of the high-salt wastewater has a high nitrogen content. , Improper treatment of high-nitrogen wastewater and direct discharge into water bodies will lead to eutrophication of water bodies and other phenomena, resulting in deterioration of water body quality. [0003] At present, there are many methods for the treatment of wastewater denitrification, such as physical method, chemical method, biological method and so on. Due to the need to add a larg...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/36C02F3/34C12R1/01C12R1/385C12R1/05
CPCC12N1/20C12N1/36C02F3/34
Inventor 赵天涛张千陈雪刘毫封丽张云茹张磊艾铄
Owner CHONGQING UNIV OF TECH
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