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Bacillus altitudinis YG045 and application thereof

A technology of bacillus uplander and bacterial suspension, applied in the direction of application, bacteria, and fungicides, can solve problems such as food safety and ecological environment threats, unsatisfactory control effects, and increased application dosage, so as to improve food and ecological environment safety , good economic and ecological benefits, the effect of reducing the amount of consumption

Active Publication Date: 2021-01-29
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term irrational use of chemical pesticides has led to the gradual increase in the resistance of pathogenic bacteria, the blind increase in the application dose, and the unsatisfactory control effect. The comprehensive control effect on diseases is 50% to 70%. threaten
In addition, due to the high cost and huge investment risk of developing safe and efficient chemical fungicides, in recent years, there are few types of new chemical fungicides registered for the control of crop bacterial diseases

Method used

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  • Bacillus altitudinis YG045 and application thereof
  • Bacillus altitudinis YG045 and application thereof
  • Bacillus altitudinis YG045 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Isolation and identification of Bacillus highlander strain YG045

[0045] (1) Separation of strain resources to be screened: randomly take 50 g of soil from the rhizosphere of an apple tree, take 10 parts of soil samples in total, mix them evenly, put 10 g of soil into a container containing 20 fine glass beads and 100 mL of sterilized deionized water In a 250mL Erlenmeyer flask, place on a shaker at 150r / min and shake at 28°C for 1h. After standing for 10 minutes, take 1 mL of the supernatant, dilute it 10 times with sterilized deionized water, draw 100 μL of each concentration gradient dilution, and spread it on the NA solid plate, and culture it at 28°C for 36 hours. Pick a single colony with an inoculation loop and streak and purify it twice on NA solid medium, transfer the purified single colony with different forms to the NA solid plate, culture it upside down at 28°C for 48h, and store it at 4°C for later use.

[0046] (2) Plate antagonism screening of biocontro...

Embodiment 2

[0051] Growth dynamics of Bacillus uplander YG045 in NB medium

[0052] Take out the YG045 strain preserved in glycerol from the -70°C ultra-low temperature refrigerator, inoculate it on the NA solid plate, and culture it at 28°C for 36 hours; use a sterilized toothpick to pick a single colony of YG045 and inoculate it into a 250mL cone containing 50mL NB liquid medium In the flask, cultured at 150r / min and 28°C for 36h as the seed liquid; inoculated the seed liquid into a 250mL Erlenmeyer flask containing 50mL NB liquid medium at a rate of 1% (v / v), 180r / min, Cultivate at 28°C for 84 hours, and measure the absorbance (OD) of the fermentation broth every 6 hours after inoculation. 600 ). Measure the cell density 60 hours after inoculation. The specific method: take samples at the above time points, perform 10-fold gradient dilution with sterilized water, draw 100 μL of each concentration gradient dilution and spread it on a NA solid plate, and incubate at 28°C for 48 hours. ...

Embodiment 4

[0057] Preparation of Bacillus highlander YG045 biocontrol agent

[0058] Take the glycerol strain of Bacillus uplander YG045 of the present invention, inoculate it on the NA solid plate by streaking, and cultivate it at 28°C for 36 hours; use a sterilized toothpick to pick a single colony of YG045 and inoculate it into a 250mL Erlenmeyer flask containing 50mL NB liquid medium cultured at 180r / min and 28°C for 16h as the seed solution; the seed solution was inoculated into a 1000mL Erlenmeyer flask containing 200mL NB medium at a ratio of 1% (v / v). Cultivate for 60 h at 28°C. Add 1.0% fatty alcohol polyoxyethylene ether, 0.2% xanthan gum, 0.3% polydimethoxyalkane, and 0.1% sodium benzoate to the fermentation broth, and use dilute hydrochloric acid to adjust the pH value to about 4.5, which is YG045 biocontrol Bacteria, then aseptically dispensed and stored at room temperature.

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Abstract

The invention belongs to the technical field of biology, and particularly relates to bacillus altitudinis YG045 and application thereof, the bacillus altitudinis YG045 is preserved in China General Microbiological Culture Collection Center on June 28th, 2020, and the preservation number of the strain is CGMCC No. 20157; the bacillus altitudinis YG045 has high antagonistic activity to xanthomonas oryzae pv. Oryzae, xanthomonas oryzae pv. Oryzae pv. Oryzae and botrytis cinerea, and has strong tolerance to common chemical bactericides for preventing and treating bacterial diseases of rice; the biocontrol microbial inoculum prepared from the strain provided by the invention has a good biocontrol effect on rice bacterial leaf blight and rice bacterial streak, can replace or reduce the use of chemical pesticides, improves the safety of food and ecological environment, and has good economic and social benefits.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a highland bacillus YG045 and its application. Background technique [0002] Rice bacterial blight (Xanthomonas oryzae pv.oryzae) and rice bacterial streak (Xanthomonas oryzae pv.oryzicola) cause rice bacterial blight and rice bacterial streak respectively, which are two major diseases in rice production in my country. Bacterial diseases, the perennial occurrence area is 10-15 million mu, and the damage area is 5-8 million mu, usually resulting in a yield loss of 5%-10%, and in severe cases, it can reach more than 50%, or even extinction. In recent years, due to the promotion of light and simplified rice planting patterns, the increase in the cultivation area of ​​two-line hybrid indica rice, the frequent exchange of germplasm resources, and the pathogenic variation of pathogenic bacteria, the two bacterial diseases have not only continued to be prevalent in southe...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00C12R1/07
CPCA01N63/22C12N1/20C12N1/205C12R2001/07
Inventor 赵延存刘凤权苗成琪徐会永孙伟波陈贤李朝辉汤宝赵杨扬徐高歌
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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