Method for highly producing short-chain fatty acid by utilizing microbial symbiotic fermentation technology

A short-chain fatty acid and symbiotic fermentation technology, which is applied in the field of microorganisms and can solve problems such as difficult applications

Active Publication Date: 2021-01-29
山东省大健康精准医疗产业技术研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The short-chain fatty acid prepared by the above method is a chemical material, which is mainly used as an external carbon source for biological nitrogen and phosphorus removal to improve the efficiency of nitrogen and phosphorus removal, and is difficult to apply in the fields of food and medicine.

Method used

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  • Method for highly producing short-chain fatty acid by utilizing microbial symbiotic fermentation technology

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Effect test

Embodiment 1

[0033] A method for high-yielding short-chain fatty acids using microbial symbiotic fermentation technology, comprising the following steps:

[0034] 1) Jerusalem artichoke pretreatment: wash 50kg of fresh Jerusalem artichoke, after the water on the surface is naturally dried, put it into a beater for beating, beat at 20Hz for 10 minutes, and obtain Jerusalem artichoke pulp;

[0035] 2) Seaweed pretreatment: Wash 50kg of seaweed, and after the moisture on the surface dries naturally, cut it into 2-5cm long and 2-5cm wide blocks, and dry it in a blast drying oven (70°C) until the water content is 10%, placed in a grinder to grind to 60 mesh, and sieved to obtain seaweed powder;

[0036] 3) Preparation of fermented pulp medium: take 10 kg of Jerusalem artichoke pulp prepared in step 1), 2 kg of seaweed powder prepared in step 2), and 0.1 kg of sodium glutamate, disperse in 87.9 kg of water, mix, and homogenize under a pressure of 20 MPa for 8 minutes , adjust the pH to 6, keep it...

Embodiment 2

[0040] A method for high-yielding short-chain fatty acids using microbial symbiotic fermentation technology, comprising the following steps:

[0041] 1) Jerusalem artichoke pretreatment: wash 50kg of fresh Jerusalem artichoke, after the water on the surface is naturally dried, put it into a beater for beating, beat at 20Hz for 10 minutes, and obtain Jerusalem artichoke pulp;

[0042] 2) Seaweed pretreatment: Wash 50kg of seaweed, and after the moisture on the surface is naturally dried, cut it into 2-5cm long and 2-5cm wide blocks, and dry it in a blast drying oven (70°C) until the moisture content is 10%, placed in a grinder to grind to 60 mesh, and sieved to obtain seaweed powder;

[0043] 3) Preparation of fermented pulp medium: take 20 kg of Jerusalem artichoke pulp prepared in step 1), 5 kg of seaweed powder prepared in step 2), and 0.5 kg of sodium glutamate, disperse in 74.5 kg of water, mix, and homogenize under a pressure of 20 MPa for 8 minutes , adjust the pH to 7,...

Embodiment 3

[0047] A method for high-yielding short-chain fatty acids using microbial symbiotic fermentation technology, comprising the following steps:

[0048] 1) Jerusalem artichoke pretreatment: wash 50kg of fresh Jerusalem artichoke, after the water on the surface is naturally dried, put it into a beater for beating, beat at 20Hz for 10 minutes, and obtain Jerusalem artichoke pulp;

[0049] 2) Seaweed pretreatment: Wash 50kg of seaweed, and after the moisture on the surface is naturally dried, cut it into 2-5cm long and 2-5cm wide blocks, and dry it in a blast drying oven (70°C) until the moisture content is 10%, placed in a grinder to grind to 60 mesh, and sieved to obtain seaweed powder;

[0050] 3) Preparation of fermented pulp culture medium: 40 kg of Jerusalem artichoke pulp prepared in step 1), 10 kg of seaweed powder prepared in step 2), and 1 kg of sodium glutamate were dispersed in 49 kg of water, mixed, homogenized under a pressure of 20 MPa for 8 min, and adjusted The pH ...

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Abstract

The invention relates to a method for highly producing short-chain fatty acid by using a microbial symbiotic fermentation technology. According to the present invention, the microbial symbiotic fermentation technology is adopted to produce the short-chain fatty acid at the high yield, the jerusalem artichoke and the seaweed with characteristics of high yield and easy obtaining are adopted as the raw materials, a food-grade material is adopted as a fermentation substrate, and the multi-bacteria symbiotic fermentation technology is adopted to produce the short-chain fatty acid, compared with theprocess of preparing short-chain fatty acids by a carbohydrate composition and pure cellulose in the prior art, the method overcomes the defect of high cost of the existing method for preparing the short-chain fatty acid, is simple to operate, safe, non-toxic, high in yield, easy for large-scale production, good in economic benefit and wide in application range, and provides a new raw material and a new method for preparing the short-chain fatty acid.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for high-yielding short-chain fatty acids by utilizing microbial symbiotic fermentation technology. Background technique [0002] Short-chain fatty acids (SCFAs), also known as volatile fatty acids (Volatilefatty acids, VFA), are organic carboxylic acids with 1 to 6 carbon atoms, and are carbohydrates (oligosaccharides, oligosaccharides, After non-starch polysaccharides, resistant starch, etc.) enter the large intestine, the end products produced by bacterial fermentation mainly include acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, and valeric acid, of which 90% to 95% The SCFAs are acetic acid, propionic acid, and butyric acid. [0003] Short-chain fatty acids are the fermentation products of intestinal microorganisms, and their effects on the health of the body have been widely studied: ①SCFAs can improve intestinal di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12P39/00C12N1/20C12N1/16C12R1/01C12R1/645
CPCC12P7/6409C12P39/00C12N1/20C12N1/16
Inventor 朱之炜苏真真高丽鹤李文靖
Owner 山东省大健康精准医疗产业技术研究院
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