Method for promoting microalgae growth and enhancing microalgae protein accumulation by utilizing yeast secreta
A secretion and protein technology, applied in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problem that microalgae technology has not become a mainstream sewage treatment technology, and achieve simple and easy operation methods. The effect of increasing the amount of synthesis and the amount of rapid synthesis
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Embodiment 1
[0040] This embodiment adopts purchased chlorella algae and yeast strains, and realizes it under medium conditions. It can be applied under the conditions of obtaining high-quality microalgae and high-quality protein under actual food-grade and non-wastewater conditions.
[0041] Bought Chlorella pyrenoidosa algae species from the Microbial Preservation Bank, take a small amount of Chlorella into 500mL M0 liquid medium, light intensity 5500-6000lx, culture at 25 degrees Celsius for 2-3 days, shake 2-3 times a day , to obtain a Chlorella culture.
[0042] If you want to obtain chlorella with high nitrogen and phosphorus removal rate, filter the chlorella culture and collect the sediment on the filter membrane; the sediment is washed 1-2 times with sterile water, and then transferred to M1 liquid culture base, illuminance 5500-6000lx, 25 degrees Celsius and 150rpm for 7 days, and the removal efficiency of nitrate, total nitrogen and total phosphorus was measured every week, and...
Embodiment 2
[0050] Chlorella algae strains separated from waste water are used, standard yeast strains are purchased, and realized under waste water conditions. It can be used to increase the removal rate of nitrogen and phosphorus in the process of wastewater treatment and at the same time increase the protein accumulation of chlorella.
[0051] 1. Take 30L of wastewater from the waterfowl breeding wastewater plant. First, after conventional isolation, remove suspended solids, large plankton, and oil in the wastewater, expose for 30 minutes, then settle for 30 minutes, and cycle 2-3 times for standby.
[0052] 2. Acquisition and cultivation of microalgae: obtain the concentrated solution by suction filtration, and collect the sediment on the filter membrane; the sediment is washed 1-2 times with sterile water, then transferred to M0 liquid medium, and illuminated at 4000-5000Lux throughout the day , cultured at 25 degrees Celsius for 3 days, shaking 3 times a day to obtain the primary cu...
Embodiment 3
[0056] Under the reactor condition, the chlorella protein accumulation is realized under the waste water condition with the chlorella algae strain and the yeast strain. It can be used to improve the removal rate of nitrogen and phosphorus in the reactor wastewater treatment process, and at the same time increase the protein accumulation of chlorella. The details are as follows: 1. In the pretreatment stage, 10-20L of wastewater is taken from the waterfowl breeding wastewater plant. First, after conventional isolation, the suspended solids, large plankton and oil in the wastewater are removed, exposed for 30 minutes, and then precipitated for 30 minutes. Repeat the treatment 2-3 times and set aside.
[0057] 2. The stage of obtaining and cultivating microalgae: obtain the concentrated solution by suction filtration, and collect the sediment on the filter membrane; after the sediment is washed 1-2 times with sterile water, it is transferred to the M0 liquid medium, and it is kep...
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