Primer group, probe group and kit for synchronously detecting multiple pathogens related to genital tract infection

Abstract

The invention discloses a primer group, a probe group and a kit for synchronously detecting multiple pathogens related to genital tract infection. The sequences of primer composition and probes are SEQIDNO: 1-45, and a fluorescent group and a quenching group are contained at the two ends of each sequence of the probe group. According to the kit disclosed by the invention, the kit comprises the primer group, the probe group, a pathogen marker standard solution as well as enzymes and reagents commonly used in PCR (polymerase chain reaction), can simultaneously detect 15 pathogens related to thegenital tract infection, and has the advantages of high reverse transcription efficiency, strong primer specificity, high sensitivity, comprehensive, rapid and accurate detection and wide applicationrange, and the detection result can be used for clinical diagnosis and treatment.

Description

technical field [0001] The invention relates to the technical field of nucleic acid amplification, in particular to a primer composition and a kit for synchronously detecting multiple pathogens related to reproductive tract infections. Background technique [0002] In recent years, the number of patients with genitourinary tract infections has been increasing. Epidemiological surveys of married women show that the prevalence of such infections has become the first among gynecological diseases. The pathogens that cause reproductive tract infections are various and complex, and there are certain asymptomatic infections, which cause many difficulties in the diagnosis and treatment of diseases. Genital tract pathogen infection usually leads to the following conditions: urinary system infection, vaginitis, pelvic inflammatory disease, etc. Reproductive tract pathogen infection will increase the incidence of male and female infertility, ectopic pregnancy, miscarriage, premature b...

AI Technical Summary

Problems solved by technology

The virus culture detection method has the characteristics of high specificity and is the "gold standard" for clinical diagnosis, but it is time-consuming, low-sensitivity, and requires high experimental operation; bacterial culture can detect drug resistance, but the sample is easy to contaminate, which affects the results. The impact is relatively large; the immunological detection of pathogens in reproductive tract infections often uses enzyme-linked immunosorbent assay. Clinical reference value; Fluorescent quantitative PCR technology has the advantages of high sensitivity, rapid detection, and relatively low cost, and has been widely used in clinical diagnosis

[0007] However, the specificity of dye-based fluorescent quantitative PCR is relatively poor, and different target products cannot be quantified separately. Multiplex fluorescent quantitative PCR is a multiple joint detection developed on the basis of it, which makes up for these shortcomings and realizes simultaneous detection in the same reaction tube. Amplification and specific detection of multiple gene sequences

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