Primer group, probe group and kit for synchronously detecting multiple pathogens related to genital tract infection

A technology for simultaneous detection of reproductive tract infection, applied in the field of primer compositions for pathogens and kits thereof, can solve the problems of low sensitivity, low sensitivity, and large impact on results, and achieves high reverse transcription efficiency, strong primer specificity, Check full effect

Active Publication Date: 2021-02-02
爱科睿特生物医疗科技(南京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The virus culture detection method has the characteristics of high specificity and is the "gold standard" for clinical diagnosis, but it is time-consuming, low-sensitivity, and requires high experimental operation; bacterial culture can detect drug resistance, but the sample is easy to contaminate, which affects the results. The impact is relatively large; the immunological detection of pathogens in reproductive tract infections often uses enzyme-linked immunosorbent assay. Clinical reference value; Fluorescent quantitative PCR technology h

Method used

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  • Primer group, probe group and kit for synchronously detecting multiple pathogens related to genital tract infection
  • Primer group, probe group and kit for synchronously detecting multiple pathogens related to genital tract infection
  • Primer group, probe group and kit for synchronously detecting multiple pathogens related to genital tract infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Preparation of a multiplex fluorescent quantitative PCR kit for the detection of 15 reproductive tract pathogens

[0046] The multiplex fluorescent quantitative PCR amplification kit used in the detection of 15 kinds of reproductive tract pathogens of the present invention, the composition of the PCR amplification kit is shown in Table 1.

[0047] Table 1 Composition list of PCR amplification kit

[0048]

[0049] The kit of the present invention can be used to detect 15 kinds of reproductive tract pathogens: herpes simplex virus type 1 (HSV1), herpes simplex virus type 2 (HSV2), group B streptococcus (SAg), atopobium (AV), common Revobacterium (PV), Megasphaerum (ME), Motonia (MB), Neisseria gonorrhoeae (NG), Gardnerella (GV), Trichomonas vaginalis (TV), Treponema pallidum (TP) , Candida albicans (CA), Ureaplasma urealyticum (UU), Chlamydia trachomatis (CT), Mycoplasma hominis (MH) nucleic acid.

[0050] The design of 15 kinds of reproductive tract path...

Embodiment 2

[0075] Embodiment 2: the detection method of multiplex fluorescent quantitative PCR kit

[0076] 1. Detection kit

[0077] The multiplex fluorescent quantitative PCR kit prepared in Example 1 for the detection of 15 kinds of reproductive tract pathogens.

[0078] Detection object

[0079] 2.1 Preparation of internal standard granule normal saline containing housekeeping gene (hereinafter referred to as "internal standard normal saline"): Measure 800mL of normal saline (0.9% sodium chloride solution) into a 1000mL volumetric flask in the reference substance preparation room in the clean work area , adding 5.0×10 9 Copies / mL pathogen plasmid or particle 1mL, dilute to 1000mL with normal saline, turn over to mix well, so that the final concentration is 5.0×10 6 copies / mL. Transfer the above solution to a 1000mL beaker and divide it for use.

[0080] 2.2 7 samples to be tested were negative

[0081]Collect cervicovaginal secretion samples such as HPV16, HPV18, HPV51, ciliat...

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Abstract

The invention discloses a primer group, a probe group and a kit for synchronously detecting multiple pathogens related to genital tract infection. The sequences of primer composition and probes are SEQIDNO: 1-45, and a fluorescent group and a quenching group are contained at the two ends of each sequence of the probe group. According to the kit disclosed by the invention, the kit comprises the primer group, the probe group, a pathogen marker standard solution as well as enzymes and reagents commonly used in PCR (polymerase chain reaction), can simultaneously detect 15 pathogens related to thegenital tract infection, and has the advantages of high reverse transcription efficiency, strong primer specificity, high sensitivity, comprehensive, rapid and accurate detection and wide applicationrange, and the detection result can be used for clinical diagnosis and treatment.

Description

technical field [0001] The invention relates to the technical field of nucleic acid amplification, in particular to a primer composition and a kit for synchronously detecting multiple pathogens related to reproductive tract infections. Background technique [0002] In recent years, the number of patients with genitourinary tract infections has been increasing. Epidemiological surveys of married women show that the prevalence of such infections has become the first among gynecological diseases. The pathogens that cause reproductive tract infections are various and complex, and there are certain asymptomatic infections, which cause many difficulties in the diagnosis and treatment of diseases. Genital tract pathogen infection usually leads to the following conditions: urinary system infection, vaginitis, pelvic inflammatory disease, etc. Reproductive tract pathogen infection will increase the incidence of male and female infertility, ectopic pregnancy, miscarriage, premature b...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/6895C12Q1/689C12Q1/6851C12Q1/14C12Q1/04
CPCC12Q1/6851C12Q1/689C12Q1/6895C12Q1/705C12Q2600/166C12Q2531/113C12Q2537/143C12Q2563/107C12Q2521/107C12Q2545/113
Inventor 闫泉熊永清王洪
Owner 爱科睿特生物医疗科技(南京)有限公司
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