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Bacillus methylotrophicus BMF 04 water suspending agent, preparation method and application thereof

A methylotrophic and bacillus-based technology, which is applied in the direction of fungicides, microbial-based methods, applications, etc., can solve the problems of late start of development and research

Active Publication Date: 2021-02-19
JIANGSU OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing microbial strains used for the development of microbial pesticides are mainly derived from terrestrial environments, and the development and research of microbial strains from the ocean started relatively late. Marine bacteria grow and reproduce quickly and have rich metabolites, which are important germplasm resources for screening resistant strains

Method used

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  • Bacillus methylotrophicus BMF 04 water suspending agent, preparation method and application thereof
  • Bacillus methylotrophicus BMF 04 water suspending agent, preparation method and application thereof
  • Bacillus methylotrophicus BMF 04 water suspending agent, preparation method and application thereof

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Experimental program
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Embodiment 1

[0043] Embodiment 1, methylotrophic bacillus (Bacillus methylotrophics) BMF 04 water suspension preparation experiment:

[0044] 1.1 Materials and methods

[0045] 1.1.1 Test strains

[0046] Methylotrophic Bacillus (Bacillus methylotrophics) BMF 04 was isolated, identified and preserved from Lianyungang sea area by our laboratory.

[0047] 1.1.2 Medium

[0048] Strain activation medium: PDA medium. Seed medium: PD medium. Fermentation medium: bran 1.0%, peanut powder 1.0%, CaCl2 1.0%, pH 7.5.

[0049] 1.1.3 Types of additives

[0050] Dispersant: sodium lignosulfonate, calcium dodecylbenzenesulfonate, sodium lauryl sulfate, calcium lignosulfonate, fatty alcohol polyoxyethylene ether 25, Tween 80; thickener: carboxymethyl Sodium cellulose, xanthan gum, soluble starch, polyvinyl alcohol, polyethylene glycol 6000; UV protector: ascorbic acid, sodium fluorescein, dextrin, humic acid, sodium alginate, urea; preservative: paraben Methyl Paraben, Ethyl Paraben, Sodium Benzoat...

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Abstract

The invention discloses a bacillus methylotrophicus BMF 04 water suspending agent, which is composed of a bacillus methylotrophicus BMF 04 fermentation broth and an auxiliary agent, and the auxiliaryagent comprises the following components in percentage by weight: 3.0% of calcium lignosulphonate, 0.20% of xanthan gum, 0.40% of potassium sorbate, 0.30% of sodium alginate, and 0.40% soybean oil. The invention also discloses a preparation method and application of the bacillus methylotrophicus BMF 04 water suspending agent. According to the invention, the water suspending agent is obtained by developing a bacillus methylotrophicus BMF 04 strain, is high in effective component, simple in preparation and processing technology, low in loss and low in cost, is a liquid preparation which is highin suspension rate, good in flowability and high in stability and obtained by researching an optimal auxiliary agent, and can be used for treating or preventing fruit ring rot and / or anthracnose.

Description

technical field [0001] The present invention relates to a kind of bacterial agent, particularly a kind of Bacillus methylotrophicus (Bacillus methylotrophicus) BMF 04 water suspension; The present invention also relates to the preparation method and application of methylotrophic Bacillus (Bacillus methylotrophicus) BMF 04 water suspension . Background technique [0002] Biological control has the advantages of no pollution to the environment, safety to humans and other organisms, long-lasting control effect, and no residue in the product. It can make up for the shortcomings caused by chemical pesticides. It is particularly important to screen more excellent strains that have strong antibacterial effects and promote crop growth. important. The existing microbial strains used for the development of microbial pesticides are mainly derived from terrestrial environments. The development and research of microbial strains from the ocean started relatively late. Marine bacteria gro...

Claims

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Application Information

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IPC IPC(8): A01N63/22A01N25/04A01P3/00C12N1/20C12R1/07
CPCA01N25/04A01N63/22C12N1/20
Inventor 马桂珍暴增海冷超凡万丹丹
Owner JIANGSU OCEAN UNIV
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