Method for preparing squalene by utilizing heterotrophic biological membrane adherent autotrophic culture of grape algae

A technology of grape algae and biofilm, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the slow growth of algae cells, the research on the production of grape algae squalene has not received much attention, and the limitation of grape algae Problems such as the medical application of squalene to achieve the effects of promoting synthesis, protecting the marine ecological environment, and increasing production

Pending Publication Date: 2021-02-23
NINGBO UNIV
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the slow growth of the algae cells, the research on the production of grape algae squalene

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing squalene by utilizing heterotrophic biological membrane adherent autotrophic culture of grape algae
  • Method for preparing squalene by utilizing heterotrophic biological membrane adherent autotrophic culture of grape algae
  • Method for preparing squalene by utilizing heterotrophic biological membrane adherent autotrophic culture of grape algae

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0020]Example 1: Comparison of squalene production between Botryococcus liquid culture and biofilm adherent culture

[0021]B. braunii in BG 11 medium, 1% CO2Concentration (volume ratio), light intensity 3000lux, temperature 25℃, liquid culture and adherent culture (figure 1 a and b). BG 11(g.L-1) The composition of the medium is as follows: potassium nitrate 1.5, magnesium sulfate heptahydrate 0.075, dipotassium hydrogen phosphate trihydrate 0.04, anhydrous calcium chloride 0.027, sodium carbonate 0.02, citric acid 0.006, ferric ammonium citrate 0.006, ethylene dichloride Sodium ferric aminetetraacetate 0.001, 5mL A5 solution, pH 7.5.

[0022]The photobioreactor (PBR) used for the B.braunii liquid suspension culture experiment consists of a flat plate reactor (length 50cm, width 20cm, thickness 1cm, volume 1L), and the light area is 0.1m2; Adherent culture is the separation of Botrytis and the culture medium, and the algae cells are placed on the mixed fiber filter membrane material with...

Example Embodiment

[0024]Example 2: Effect of different carbon sources and their concentrations on the growth of Botryococcus under heterotrophic conditions

[0025]Botrytis was cultivated at an additive concentration of 2.0 g.L under the same light intensity (3000lux) and the same conditions as in Example 1.-1In the different organic carbon culture media, the organic carbon sources are glycerol, glucose and acetic acid. The training results are asimage 3 shown in a. The results showed that Botryococcus algae grows best in the medium where the organic carbon source is glucose, and the biological yield is 1.01g.L-1.d-1.

[0026]Under the condition that the organic carbon source is determined to be glucose, set different carbon source concentrations as 0.5, 1.0, 2.5, 5.0 g.L respectively-1To cultivate. The results showed that Botryococcus in the glucose concentration of 2.5g.L-1The best growth rate is 1.21g.L-1.d-1.

Example Embodiment

[0027]Example 3: Adjusting the N concentration to increase the yield of squalene from Botryococcus under autotrophic conditions

[0028]Under the same conditions as in Example 1, Botryococcus algae was cultured adherently in BG 11 medium with different initial N concentrations for 7 days. The initial N concentration was set to normal BG 11 (1.5g.L-1), 1 / 2N(0.75g.L-1), 1 / 4N(0.375g.L-1), 1 / 8N(0.1875g.L-1), 0N.

[0029]The result isFigure 4 As shown, under the conditions of normal BG 11, 1 / 2N, and 1 / 4N of N concentration, Botryococcus is not much different, and the biological yield is 5.24, 5.08, 4.86g.m, respectively.-2.d-1, While the growth of algae cells is inhibited under the conditions of N concentration of 1 / 8N and 0N, and the biological yields are 3.32, 2.83g.m, respectively-2.d-1.

[0030]Regarding the content of squalene, the content of squalene under low-N conditions is significantly increased, and under different N concentration conditions (normal BG11, 1 / 2N, 1 / 4N, 1 / 8N, ON), the squ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Login to view more

Abstract

The invention provides a method for preparing squalene by culturing grape algae. The method comprises the following steps of: optimizing a culture mode of the grape algae for producing squalene, so asto improve the yield of squalene in the grape algae culture process; and further improving the medical application of grape algae squalene, reducing environmental destruction caused by squalene application and development, and making up the defects in the prior art. The invention firstly provides a method for culturing grape algae, and the method comprises the following steps of: adding glucose into a BG11 culture medium to culture the grape algae, so as to propagate the algae. The invention further provides a method for improving the yield of squalene by the grape algae, and the method comprises the following steps of: culturing the grape algae in the BG11 culture medium, and reducing the nitrogen concentration in the BG11 culture medium. According to the method disclosed by the invention, synthesis of squalene is promoted under the condition that growth of the grape algae is not influenced, the yield of squalene with medical value is further improved, the application prospects of grape algae squalene products are improved, and the catching and killing amount of sharks is expected to be reduced, so that the marine ecological environment is protected.

Description

technical field [0001] The invention belongs to the technical field of botrytis propagating cultivation and high-value product production thereof, and in particular relates to a method for preparing squalene by cultivating botrytis. Background technique [0002] As a biosynthetic precursor of plant and animal steroids, squalene is an unsaponified colorless oily triterpenoid hydrocarbon with significant antioxidant and antibacterial activities, and is widely used in biomedicine and food supplements , high-quality cosmetics and other industries. In 2020, according to British media reports, some COVID-19 vaccine candidates contain squalene. In 2014 alone, the global squalene market demand reached 260,000 tons, and the market value was as high as 240 million US dollars. [0003] However, the current commercial source of squalene is mainly the liver oil of deep-sea sharks, but due to the protection of marine wildlife and fishery resources, the continuous supply and future avail...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/12C12P5/00C12R1/89
CPCC12N1/12C12P5/026
Inventor 程鹏飞周成旭严小军褚蕊蕊刘天中王海霞
Owner NINGBO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap