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Bionic nano-emulsion and preparation method and use of bionic nano-emulsion

A technology of biomimetic nanometer and nanoemulsion, which is applied in the field of biomimetic nanoemulsion and its preparation, can solve the problems of limited targeting ability of tumor sites, etc., and achieve good application prospects, easy industrial production, and convenient operation

Active Publication Date: 2021-02-26
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a biomimetic nanoemulsion and its preparation method and application, aiming to solve the problem of the existing immune checkpoint inhibitors being diffusely distributed throughout the body and having limited targeting ability to tumor sites

Method used

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  • Bionic nano-emulsion and preparation method and use of bionic nano-emulsion

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Embodiment 1

[0062] The preparation steps of the biomimetic nanoemulsion (albumin-perfluorotributylamine-huaptorphyrin sodium wrapped in cell membrane with high expression of PD-1) in this example are as follows:

[0063] Mix human serum albumin and water, and the concentration of the mixed solution is 30 mg / mL, including 240 mg of human serum albumin, and then slowly add 300 μL of perfluorinated compound and stir. The above mixed solution was ultrasonically treated with a probe at 260W for 7 minutes to obtain an albumin-perfluorinated compound nanoemulsion.

[0064] Add 20 mg / mL of Sinoporphyrin sodium into the albumin-perfluorinated compound nanoemulsion and stir for 12 hours. The mass ratio of albumin to Sinoporphyrin sodium is 30:400 to obtain albumin bound to the surface of Sinoporphyrin sodium. - Perfluorochemical nanoemulsions.

[0065] Extract the cell membrane with high expression of PD-1: Digest HEK293T cells with trypsin, centrifuge at 1500rpm for 5min to get the cells, wash 3 ...

Embodiment 2

[0069] Toxicity evaluation of photodynamic therapy and immunotherapy combined therapy on 4T1 tumor cells

[0070] The standard MTT method was used to evaluate the effect of co-treatment of photodynamic therapy and immunotherapy on the survival rate of 4T1 cells. 4T1 cells at 5×10 per well 3 Density seeded into 96-well plates and placed at 37°C, 5% CO 2 Cultivate for 24 hours under the same conditions. Next, aspirate the old medium in the 96-well plate, and add DMEM medium containing 200ng / mL DVDMS, PFTBA@HSA-DVDMS(PHD), and PFTBA@HSA-DVDMS@PD-1NVs(PHD@PM) respectively. After continuing to culture for 2 hours, suck out the old medium in the 96-well plate, add 100 μL of DMEM medium to each well, and then irradiate the cells with a 635 nm laser for 5 minutes at a power of 200 mW / cm to each well of the light well. 2 . After irradiation, suck out the old medium in the 96-well plate, add 100 μL of medium solution containing 5 mg / mL MTT to each well, and continue culturing for 12...

Embodiment 3

[0073] Evaluation of the effect of low-frequency ultrasound irradiation on promoting the accumulation of biomimetic nanoemulsions in subcutaneous tumors

[0074] Female Balb / c mice (3 weeks, 15-20g), subcutaneously inject 2×10 6 4T1 tumor cells were used to establish a mouse subcutaneous tumor model. When the bilateral tumor volume exceeds 100mm 3 , a fluorescence imaging experiment was performed. At 1, 4, 8, 12, 24, 48, and 72 hours after the tail vein injection of the drug in the tumor-bearing mice, the changes of the tumor accumulation were observed by fluorescence imaging of Sinoporphyrin sodium and analyzed quantitatively. see results image 3 .

[0075] image 3 In a, with the prolongation of time after administration, the accumulation of the drug in the tumor gradually increases, and the fluorescence at the tumor site decays slowly at 24-72 hours, indicating that there is a short residence time. image 3 b is the fluorescence intensity value in the isolated organs...

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Abstract

The invention discloses a bionic nano-emulsion and a preparation method and use of bionic nano-emulsion. The bionic nano-emulsion comprises an albumin perfluorinated compound nano-emulsion with a surface combined with sinoporphyrin sodium and a PD-1 high-expression cell membrane wrapping the albumin perfluorinated compound nano-emulsion with the surface combined with the sinoporphyrin sodium. Thebionic nano-emulsion can simultaneously realize fluorescence imaging and photodynamic therapy and immunotherapy combined collaborative treatment of tumors, and thus has a good application prospect inthe field of tumor diagnosis and treatment. Meanwhile, a preparation process is simple, an operation is convenient, complex and expensive devices are not needed, and industrial production is easy to realize.

Description

technical field [0001] The invention relates to the field of medical nanomaterials, in particular to a biomimetic nanoemulsion and its preparation method and application. Background technique [0002] Photodynamic therapy of tumors activates photosensitizing drugs by light, thereby converting oxygen in tissues into toxic singlet oxygen, killing tumor cells, and achieving therapeutic purposes. Photodynamic therapy consists of three main elements: a photosensitizer, light, and oxygen. Among them, oxygen is used as the reaction substrate generated by singlet oxygen, and its concentration significantly affects the efficiency of photodynamic therapy. However, the deep part of solid tumors has varying degrees of hypoxia, which limits the efficacy of photodynamic therapy. A variety of liquid perfluorocarbons (such as perfluorohexane, perfluorotributylamine, perfluoro-15-crown-5-ether, etc.) have a high affinity for oxygen. Encapsulated in nanocarriers, it can realize the slow re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/107A61K47/64A61P35/00
CPCA61K41/0071A61K9/1075A61K47/643A61P35/00
Inventor 黄鹏张一帆廖云燕林静
Owner SHENZHEN UNIV
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