Artificial dermis and preparation method and application thereof

An artificial and dermal technology, applied in medical science, prosthesis, etc., can solve the problems of difficulty in ensuring the uniformity of preparations, affecting the effect of wound repair, and sudden release concentration of preparations, so as to avoid concentration of release concentration and promote cell proliferation. Growth and migration, effect of uniform concentration

Inactive Publication Date: 2021-02-26
SHENZHEN QIKANG MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practical applications, it is found that the uniformity of the preparation in the artificial dermis prepared in this way is difficult to ensure, so that the preparation is prone to "burst release" or excessively concentrated release concentration during the degradation process of the collagen sponge layer, which affects The effect of wound repair

Method used

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  • Artificial dermis and preparation method and application thereof
  • Artificial dermis and preparation method and application thereof
  • Artificial dermis and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0052] This embodiment provides a kind of artificial leather, such as figure 1 As shown, the artificial dermis includes a first collagen sponge layer 101 , a hydrogel biofilm layer 102 and a second collagen sponge layer 103 arranged in sequence. The first collagen sponge layer 101 and the second collagen sponge layer 103 are obtained by cross-linking collagen materials and chondroitin sulfate, and a certain amount of broad-spectrum antibacterial agent polyhexamethylene biguanide hydrochloride (PHMB) is mixed therein. The hydrogel biofilm layer 102 is a polycarboxymethyl chitosan-sodium alginate hydrogel scaffold doped with calcium polyphosphate particles. The particle diameter of the calcium polyphosphate particles is 100-300nm, and the repeating unit number of the phosphate group (Pi) is 40-100Pi. During the use of the artificial dermis, with the degradation of the hydrogel scaffold, the calcium polyphosphate particles are slowly released to the wound surface to provide meta...

Embodiment 2

[0060] This embodiment provides a method for preparing artificial dermis. The difference between this preparation method and Example 1 is that the components and contents of each step are different, as follows:

[0061] S1. Preparation of hydrogel biofilm: Weigh a certain amount of carboxymethyl chitosan into a small beaker, add deionized water and stir to dissolve to obtain a carboxymethyl chitosan aqueous solution with a concentration of 60 mg / mL. The same method was used to prepare a sodium polyphosphate aqueous solution with a concentration of 20 mg / mL and a gelatin aqueous solution with a concentration of 50 mg / mL. Take 5mL of sodium polyphosphate aqueous solution and add it into 10mL carboxymethyl chitosan aqueous solution through high-speed airflow atomization, and then add 15mL of MgCl with a concentration of 20mg / mL through high-speed airflow atomization 2 Aqueous solution, adjusted pH to 10 with 6M NaOH, stirred for 4h. Then, 10 mL of gelatin aqueous solution was ad...

Embodiment 3

[0066] This embodiment provides a method for preparing artificial dermis. The difference between this preparation method and Example 1 is that the components and contents of each step are different, as follows:

[0067] S1. Preparation of hydrogel biofilm: Weigh a certain amount of carboxymethyl chitosan into a small beaker, add deionized water and stir to dissolve to obtain a carboxymethyl chitosan aqueous solution with a concentration of 30 mg / mL. The same method was used to prepare a sodium polyphosphate aqueous solution with a concentration of 10 mg / mL and a sodium alginate aqueous solution with a concentration of 60 mg / mL. Take 5mL of sodium polyphosphate aqueous solution and add it into 10mL carboxymethyl chitosan aqueous solution through high-speed airflow atomization, and then add 15mL of CaCl with a concentration of 10mg / mL through high-speed airflow atomization 2 Aqueous solution, adjusted pH to 10 with 6M NaOH, stirred for 4h. Then, 10 ml of sodium alginate aqueous...

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Abstract

The invention provides artificial dermis and a preparation method thereof. The artificial dermis comprises a first collagen sponge layer, a hydrogel biofilm layer and a second collagen sponge layer which are arranged in sequence; and the hydrogel biofilm layer comprises a hydrogel support loaded with a therapeutic agent. Compared with a mode that the therapeutic agent is directly blended into thecollagen sponge layer of the artificial dermis or added into a polymer support through electrostatic spinning, the hydrogel support with the middle core layer is adopted as a carrier in the scheme; the therapeutic agent can be evenly distributed in the horizontal plane of the support; the long-term slow-release effect can be achieved along with degradation of the hydrogel support; in addition, theconcentration of therapeutic agent particles at different positions of a wound surface is uniform; the phenomenon of burst release or release concentration centralization is avoided; and the functions of the therapeutic agent are better reserved and played.

Description

technical field [0001] The invention relates to the technical field of tissue engineering regeneration, in particular to an artificial dermis and its preparation method and application. Background technique [0002] Exposed bone wounds and chronic wounds are usually difficult to heal, and the treatment cycle is long. Improper treatment may lead to amputation or even endanger the patient's life, which brings a huge economic burden to the patient and society, and seriously affects the quality of daily life of the patient. The traditional treatment method for exposed bone wounds and chronic wounds is skin flap / piece transplantation, but its disadvantage is that it will cause secondary damage to the donor site and a possible bloated wound appearance. And when encountering large-scale wounds, it is often impossible to perform flap / piece transplantation due to limited skin sources or poor systemic or local conditions. In recent years, the development of tissue engineering and bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/02A61L27/20A61L27/52A61L27/54A61L27/56A61L27/58A61L27/60
CPCA61L27/24A61L27/20A61L27/02A61L27/60A61L27/54A61L27/52A61L27/56A61L27/58A61L2300/232A61L2300/404A61L2300/602C08L89/00C08L5/08
Inventor 王丹妍谭荣伟梁嘉美许孟强郭远军刘曦陈滢滢毛诗哲马丽佘振定
Owner SHENZHEN QIKANG MEDICAL DEVICES
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