Application of bacillus licheniformis W10 bacterial suspension in inducing nectarine fruit to resist brown rot
A technology for Bacillus licheniformis and peach brown rot, applied in the field of microorganisms, can solve problems such as 3R", and achieve the effects of increasing expression, simple application method and improving activity
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Embodiment 1
[0027] Embodiment 1, Bacillus licheniformis W10 are to the control effect of nectarine fruit brown rot;
[0028] In order to study the control effect of Bacillus licheniformis W10 on peach brown rot of nectarine fruit, nectarine fruit was treated with Bacillus licheniformis W10. Set up 3 treatment groups: clear water treatment (CK), inoculation of peach brown rot treatment (M.fructicola), spraying W10 bacterial solution 1dpi inoculation of peach brown rot treatment (W10+M.fructicola), each nectarine fruit was sterilized with The needle pierces four 5mm deep holes evenly at the equator, so that the mushroom cake with a diameter of 6mm is just covered. Three peach fruits were used for each treatment, moistened with gauze, and placed in a 25°C, 12h light / 12h dark light incubator. This experiment was repeated 3 times. Culture 2dpi to measure the diameter of lesion on the surface of peach fruit and record it. The result is as figure 1Shown, the nectarine fruit of clean water tr...
Embodiment 2
[0029] Embodiment 2, bacillus licheniformis W10 is to the impact of nectarine fruit oxidative damage;
[0030] Oxidative damage caused by plant pathogens can adversely affect plant cell membranes. MDA content and conductivity are important indicators of membrane lipid peroxidation, cell death, and membrane permeability. Therefore, MDA content and electrical conductivity were measured in nectarine fruits treated with Bacillus licheniformis W10 at different time intervals (0, 1, 2, 3, 4 dpi). Set up 4 treatment groups: water treatment (Water), W10 bacterial suspension treatment (W10), spraying clean water 1dpi to inoculate peach brown rot treatment (Water+M.fructicola), spraying W10 bacterial suspension 1dpi to inoculate peach brown rot treatment (W10+M.fructicola), each nectarine fruit was evenly pierced with 4 holes of 5 mm deep at the equator with a sterilized needle, so that the bacterium cake with a diameter of 6 mm was just covered. Three peach fruits were used for each ...
Embodiment 3
[0034] Embodiment 3, Bacillus licheniformis W10 are to the influence of nectarine fruit active oxygen;
[0035] Reactive oxygen species (ROS) production is often associated with plant cell death and increased plant susceptibility to pathogenic bacteria, H 2 o 2 Is an important active oxygen. In order to accurately determine the effect of Bacillus licheniformis W10 on the active oxygen of nectarine fruit, 4 treatment groups were set up: water treatment (Water), W10 bacterial suspension treatment (W10), spraying water 1dpi to inoculate peach brown rot treatment (Water+M fructicola), spraying W10 bacterial suspension 1dpi to inoculate peach brown rot fungus treatment (W10+M.fructicola), each nectarine fruit is evenly pierced with a sterilized needle to puncture four 5mm deep holes at the equator, so that the bacteria with a diameter of 6mm The pie is just covered. Three peach fruits were used for each treatment, moistened with gauze, and placed in a 25°C, 12h light / 12h dark li...
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