Acyl coenzyme A oxidase 2 gene RKACOX2 and application thereof

Abstract

The invention discloses an acyl coenzyme A oxidase 2 gene RKACOX2, the nucleotide sequence of the acyl coenzyme A oxidase 2 gene RKACOX2 is shown as SEQ ID NO: 1, and the amino acid sequence coded bythe acyl coenzyme A oxidase 2 gene RKACOX2 is shown as SEQ ID NO: 2. The gene is separated from Rhodosporidium kratochvilovae YM25235, the gene is transferred into the Rhodosporidium kratochvilovae YM25235, experimental results show that overexpression of the RKACOX2 gene can cause improvement of the transcription level of the gene in cells to a certain extent, and overexpression of the RKACOX2 gene can promote synthesis of all components of total carotenoid and carotenoid. Microorganisms are modified through a genetic engineering means, the yield of microorganism carotenoid can be increased,and a foundation is laid for large-scale commercialized production of the carotenoid.

Description

technical field

[0001] The invention belongs to the field of biotechnology and genetic engineering, and relates to an acyl-CoA oxidase 2 gene RKACOX2 And application thereof; Specifically relate to from yeast --- rhodosporidium ( Rhodosporidium kratochvilovae ) cloned the gene in YM25235, connected it with the carrier and transferred it into yeast cells to increase the expression level of the gene and ultimately promote the synthesis of carotenoids. Background technique

[0002] Carotenoid is a general term for terpenoids and their derivatives composed of isoprenoid as the basic unit. So far, humans have discovered more than 600 natural carotenoids in nature; studies have shown that most carotenoids are compounds containing forty carbons, and their main chain is composed of 4 isoprene units. According to the molecular structure Different elements are divided into two categories: the molecular structure contains only two elements of carbon and hydrogen, such as β-carotene,...

Images