Application of bile acid complex microbial inoculant in preparation of pelteobagrus fulvidraco feed additive
A feed additive and compound bacterial agent technology, applied in the application, animal feed, animal feed and other directions, can solve the problems of time-consuming, drugs, labor and money, increase breeding consumption and cost, increase breeding cost, etc., and achieve liver protection. The effect of gallbladder, liver and gallbladder injury, physical enhancement and anti-stress ability, no toxic and side effects
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Embodiment 1
[0028] 1. Screening and identification of Parabacteroides distirii LCG-06
[0029] 1. Screening of Parabacteroides distirii LCG-06
[0030] Take intestinal feces from the intestines of pigs, mix 10g of feces and 100mL sterile water, filter to remove impurities, and make a feces dilution, and then continue to add sterile water to the dilution to prepare 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 diluent; then take 10 -2 、10 -3 、10 -4 、10 -5 Spread the dilution on soybean casein agar medium (TSA) in a medium containing 80% N 2 , 10% CO 2 and 10%H 2 Under an anaerobic environment, cultured at 37°C for 4 days, selected obvious colonies and purified them several times to obtain a single colony, which was named LCG-06, and then placed in soybean casein agar slant medium for refrigerated storage. The formula of described TSA culture medium is:
[0031] Tryptone 15.0g, soytone 5.0g, sodium chloride 5.0g, agar 15.0g, sterile water 1000mL, pH 7.3±0.2.
[0032] Such as figure 1 As s...
Embodiment 2
[0062] 70 mu of yellow catfish are cultured in fish ponds in Huzhou, Zhejiang, such as image 3 As shown, many fish with big belly and bulging appeared in the breeding, and a large number of deaths occurred. The farmers had to clean up more than 70 catties of dead fish every day. However, the veterinarian's anatomical diagnosis found that the yellow catfish was not only infected with trichomonas, but also had severe hepatobiliary syndrome leading to necrosis in the liver. The two problems caused the secondary infection of bacteria to cause the yellow catfish's abdominal cavity. Case.
[0063] Composite bacterial agent of the present invention is mixed in the feeding feed of yellow catfish with the ratio of 2mL / kg feed, feeds every day 3 times, after feeding for 1 week, the dead number of yellow catfish in the pond becomes less, after feeding for 2 weeks, in the pond There are no more fish with big bellies, and after dissection, the liver of the fish is ruddy and elastic, and ...
Embodiment 3
[0065] 30 mu of yellow catfish were cultured in a fishpond in Jiangsu, and 2 mu of yellow catfish were randomly selected to add the compound bacterial agent of the present invention to the special feed for yellow catfish. During the breeding process, it was found that the top of the head of the yellow catfish that did not use the compound bacterial agent was congested, the skin on the head was festered, and the top of the head of the fish was severely perforated, cracked, and even the skull was hollowed out, forming a long and narrow cavity, exposing the brain tissue ( Figure 5 ), diagnosed as split head disease. However, during the same breeding period, the yellow catfish treated with the compound bacterial agent only had slight swelling on the back, and there was no obvious damage ( Figure 6 ), which shows that the compound bacterial agent of the present invention can enhance the physique and anti-stress ability of the yellow catfish, also has a good bacteriostasis effect...
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