Preparation method and application of exosome for expressing ACE2 protein

An exosome and protein technology, applied in the medical field, can solve the problems of inability to use on a large scale and limited plasma sources.
CN112430581AActive Publication Date: 2021-03-02SUZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU UNIV
Publication Date
2021-03-02

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Abstract

The invention discloses a preparation method of an exosome for expressing ACE2 protein. The preparation method comprises the following steps: constructing an hMSC human mesenchymal stem cell line forstably expressing human ACE2 protein; and extracting exosome rich in ACE2 protein from a culture supernatant of an hMSC human bone marrow mesenchymal stem cell line through an ultracentrifugation method. The exosome can neutralize new coronavirus, and has treatment and prevention potential on the novel coronavirus. Therefore, the exosome for expressing the ACE2 protein, which is prepared by the method, can be applied to the preparation of anti-novel coronavirus SARS-CoV2 medicines.
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Description

technical field

[0001] The invention belongs to the field of medical technology, and relates to a preparation method and application of exosomes expressing ACE2 protein. Background technique

[0002] The novel coronavirus belongs to the genus Coronavirus, and its basic structure consists of M protein, S protein, N protein, E protein, genomic single-stranded RNA and envelope. Studies have shown that the mechanism of SARS-CoV-2 infecting the human body is similar to that of the S protein, which combines with the ACE2 protein of human cells to mediate the virus infecting the host.

[0003] Angiotensin-converting enzyme 2 (ACE2) is a homologue of carboxypeptidase ACE. Under physiological conditions, ACE converts angiotensin I (Ang I) to angiotensin II (Ang II), thereby activating renin-vascular Tensin system (RAS), and ACE2, as a negative regulator of RAS, negatively regulates the activation of RAS by cutting and inactivating Ang II, thereby regulating the homeostasis of the bo...

Claims

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