Microfluidic chip for separating neuroblastoma CTC and capture method thereof

A technology of neuroblastoma and microfluidic chip, which is applied in the field of microfluidic chip for separating circulating tumor cells (CTC), which can solve the problems of poor and intractable prognosis, and achieve the goal of reducing detection cost, reducing operation steps and high activity Effect
CN112481081APending Publication Date: 2021-03-12SHENZHEN CHILDRENS HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHENZHEN CHILDRENS HOSPITAL
Publication Date
2021-03-12

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Abstract

The invention discloses a microfluidic chip for separating neuroblastoma (CTC) and a capture method thereof, relates to a microfluidic chip for separating CTC, and aims to enable CTC cells to be quickly enriched and captured in the chip. The method provided by the invention realizes one-step separation and capture of the neuroblastoma CTC from whole blood based on the critical sorting size of fluid mechanics in physics and the antigen-antibody affinity effect in biochemistry, reduces the operation steps, and improves the capture efficiency and cell activity of the neuroblastoma CTC. The methodhas the advantages that one-step operation is achieved, and complex pretreatment is not needed; 2, the capture efficiency and purity of CTC are improved through two-stage separation; 3, the CTC cellswith high efficiency and high activity are finally obtained under the dual capture action; 4, the cells can be put under the microscope for observing and detecting.
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Description

technical field

[0001] The invention relates to a microfluidic chip for isolating circulating tumor cells (CTCs), in particular to a microfluidic chip for isolating neuroblastoma CTCs and a capture method thereof. Background technique

[0002] Neuroblastoma (NB) is the most common extracranial solid tumor in infants and young children, accounting for 8% to 10% of childhood malignancies. Neuroblastoma is a group of diseases with very different clinical manifestations and prognosis, ranging from tumor dissemination, metastasis, death of children, to tumor maturation into benign ganglioneuroma or spontaneous regression and other clinical outcomes. The adrenal gland is the most common primary site, followed by the abdominal sympathetic ganglion, thoracic sympathetic ganglion, cervical sympathetic ganglion, and pelvic sympathetic ganglion. About 1% of patients failed to find the primary tumor. Neuroblastoma can metastasize to lymph nodes, bone marrow, bone, dura mater, orbit, li...

Claims

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