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Composition containing lactic acid bacteria fermentation product for whitening and application of composition

A technology of lactic acid bacteria fermentation and composition, which is applied to the field of compositions containing lactic acid bacteria fermentation products, can solve the problems of accelerated skin, dull skin, aging and the like, and achieves the effects of improving skin quality and reducing accumulation

Pending Publication Date: 2021-03-12
锦乔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with age, lack of sleep, ultraviolet rays, life pressure and other external or internal factors continue to stimulate the skin, the production of melanin often exceeds the speed that the skin can metabolize and accelerate skin aging, resulting in dull skin or dark spots, skin cancer and other diseases

Method used

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  • Composition containing lactic acid bacteria fermentation product for whitening and application of composition
  • Composition containing lactic acid bacteria fermentation product for whitening and application of composition
  • Composition containing lactic acid bacteria fermentation product for whitening and application of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1: Morphology and General Properties of Lactic Acid Bacteria Strains

[0058] According to the 16S rDNA sequence analysis and the API bacterial identification system analysis results, the taxonomic characteristics of the strains were confirmed. Lactic acid bacteria strain of the present invention is listed in table 2 in detail in the feature on morphology and general property:

[0059] Morphological and general property characteristics of the lactic acid bacteria strain of the present invention

[0060]

[0061]

[0062]

[0063]

Embodiment 2

[0064] Embodiment 2: collection and preservation of lactic acid bacteria fermentation product

[0065] The fermented products produced by the fermentation of the lactic acid bacteria strains of the present invention are Bifidobacterium breve Bv-889 strains, Bifidobacterium longum subsp. At least one of the 688 strain, the Lactobacillus salivarius AP-32 strain and the Lactobacillus paracasei GL-156 strain is fermented to produce its fermented product, and then through steps such as centrifugation, filtration, and heat sterilization, the fermented liquid is finally purified. According to the requirement, the fermented liquid can be further dried into lactic acid bacteria fermented product powder. The powder or aqueous solution of the fermentation broth can be stored at normal temperature. The Bifidobacterium breve Bv-889 strain, the Bifidobacterium longum subsp. infantis BLI-02 strain, the Bifidobacterium animal CP-9 strain and the Bifidobacterium bifidum Bf-688 strain used in ...

Embodiment 3

[0066] Embodiment 3: analysis of the ability to inhibit melanin formation of lactic acid bacteria fermentation product

[0067] Melanoma cells (B16-F10) are widely used in the analysis of cell models of melanin production. In experiments, ultraviolet light irradiation can be used to induce the formation of melanin. Melanoma cells can also induce the activation of melanoma cells by adding melanocyte-stimulating hormone α-MSH (melanocyte-stimulating hormones), and then produce melanin. The generated melanin can be measured using OD 400nm to be quantified. Briefly, cells were lysed, melanin was extracted, and OD was analyzed using a spectrophotometer 400nm Absorbance value, the greater the absorbance value, the more melanin produced by melanoma cells.

[0068] This experiment is the Bifidobacterium breve Bv-889 bacterial strain of the present invention, Bifidobacterium longum subsp. Bacillus AP-32 strain or Lactobacillus paracasei GL-156 strain fermentation product powder 2.5...

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Abstract

The invention provides a composition containing a lactic acid bacteria fermentation product and used for whitening and an application of the composition. Specifically, the invention provides a fermentation product produced by fermenting at least one of the following lactic acid bacteria: a bifidobacterium breve Bv-889 strain, a bifidobacterium longum infant subspecies BLI-02 strain, a bifidobacterium animalis CP-9 strain, a bifidobacterium bifidum Bf-688 strain, a lactobacillus salivarius AP-32 strain and a lactobacillus paracasei GL-156 strain. A fermented product of the lactic acid bacteriahas a skin whitening effect and is present in the form of a food composition or a cosmeceutical composition.

Description

technical field [0001] The present invention relates to a composition and its use, especially a composition containing lactic acid bacteria fermented product for whitening effect and its use. Background technique [0002] It is the nature of human beings to love beauty, and it is the dream of many oriental women to have fair and bright skin. Long-term exposure of the skin to pollutants, carcinogens and ultraviolet rays will inevitably cause damage to the skin. In order to reduce the damage of these substances to the skin, the melanocytes in the skin will produce melanin. Melanin can absorb most of ultraviolet rays, thereby protecting and alleviating acute and chronic skin damage caused by ultraviolet light. [0003] Melanin accumulates in the skin, and skin cells will produce a cell metabolism mechanism to remove melanin in the basal layer. The principle is mainly through the metabolism of skin cells to replace the old melanin accumulation cells with new skin cell prolifer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K8/99A61Q19/02A23C9/127A23C9/16A23C19/032A23F3/00A23F5/00A23G3/36C12R1/01C12R1/225
CPCC12N1/20A61K8/99A61Q19/02A23C9/127A23C9/16A23C19/032A23F3/00A23F5/00A23G3/36A61K2800/85A23V2400/515A23V2400/517A23V2400/519A23V2400/533
Inventor 谢佩珊郭仲伟蔡宜钧何协勋郭易纬林家弘
Owner 锦乔生物科技有限公司