BCG_0349 gene-deleted recombinant bacillus calmette-guerin vaccine as well as construction method and application thereof

A technology of recombinant BCG and gene deletion, applied in the direction of microorganism-based methods, applications, genetic engineering, etc., can solve the problems of accelerating tuberculosis, not superior to BCG, and unable to achieve immune protection functions, etc., to achieve the goal of promoting the secretion of inflammatory cytokines Effect
CN112481184AActive Publication Date: 2021-03-12HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2021-03-12

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Abstract

The invention discloses a gene-deleted recombinant bacillus calmette-guerin vaccine, and belongs to the technical field of zoonosis prevention and treatment. The gene deleted by the strain is a BCG_0349 gene with a nucleotide sequence shown as SEQ ID NO: 1. A homologous exchange substrate containing upstream and downstream homologous arms of the BCG_0349 gene is transduced into a bacillus calmette-guerin vaccine genome in a thermo-sensitive phage transducing mediated homologous recombination mode to knock out the BCG_0349 gene, the strain is successfully constructed, and it is confirmed for the first time that a deleted strain can promote expression and secretion of inflammatory cytokines of the organism and is weakened in toxicity; and specifically, the reduction of the forming ability ofthe soxhlet structure and the reduction of the phenotype and intracellular survival ability of small bacterial colonies are easier for removal of organisms, and the vaccine has good application potential in the related fields of tuberculosis diagnosis of human and animals, vaccine and drug development and the like.
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Description

technical field

[0001] The invention belongs to the technical field of prevention and treatment of zoonoses, and relates to BCG, in particular to a gene-deleted recombinant BCG, and also relates to a construction method and application of the strain. Background technique

[0002] Bovine tuberculosis is a chronic consumptive zoonotic disease that occurs in a variety of animals and humans, and has brought huge economic losses to the world's cattle industry and public health. Mycobacterium bovis (M.bovis) is the main pathogenic bacterium that causes bovine tuberculosis, and it has 99.95% homology at the gene level with the main pathogenic bacterium of human tuberculosis, Mycobacterium tuberculosis (M.tb) , so the infection spectrum crosses each other, and both can infect almost all vertebrates, including humans. For this reason, the only official vaccine currently used to prevent human tuberculosis is an attenuated strain of Mycobacterium bovis - Mycobacterium bovis Bacille Ca...

Claims

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