Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing collagen peptide by using cod skin as raw material

A collagen peptide and cod technology, which is applied in the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problems of incomplete molecular weight distribution of peptides, incomplete odor adsorption, and high protein loss rate, and achieve anti- Strong oxidizing ability, eliminating odor, high biological activity effect

Active Publication Date: 2021-03-12
山东恒鑫生物科技有限公司
View PDF6 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the collagen peptides in my country are in the primary stage, and the collagen peptide products extracted from aquatic products are prone to produce unpleasant fishy smell, and the color is deep, the molecular weight distribution of peptides is not concentrated, and the extraction rate is low.
In the prior art, activated carbon is mostly used for odor adsorption, but this method is not thorough for odor adsorption, and the protein loss rate is high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) After cleaning the cod skin, add 0.6% sodium hydroxide aqueous solution to degrease for 3 hours, then add 20mL of 10% sodium chloride-citric acid aqueous solution per 1g for gradient soaking, the first gradient is chlorine The mass ratio of sodium chloride to citric acid is 1:0.3, soak for 2 hours, the mass ratio of the second gradient sodium chloride to citric acid is 2:0.3, after soaking for 2 hours, slowly add hydrogen carbonate with a concentration of 0.15g / mL Calcium aqueous solution (the molar ratio of calcium bicarbonate to citric acid is 3:2), continue to soak for 3 hours after the dropwise addition, filter after soaking, wash the fish skin with deionized water, and dry the fish skin after the surface moisture, every 1g Add 10mL of 7g / mL acetic acid solution and sodium alginate accounting for 0.6% of the mass of cod skin to the cod skin to swell for 12h, beat the pulp, adjust the pH to 7.5, and obtain the fish skin slurry for later use;

[0021] (2) Add 1200...

Embodiment 2

[0023] (1) After cleaning the cod skin, add 0.6% sodium hydroxide aqueous solution to degrease for 3 hours, and then add 25mL of 10% sodium chloride-citric acid aqueous solution per 1g for gradient soaking, the first gradient is chlorine The mass ratio of sodium chloride to citric acid is 1:0.5, soak for 2 hours, the mass ratio of the second gradient sodium chloride to citric acid is 2:0.5, after soaking for 2 hours, slowly add hydrogen carbonate with a concentration of 0.20g / mL Calcium aqueous solution (the molar ratio of calcium bicarbonate to citric acid is 3:2), continue to soak for 3 hours after the dropwise addition, filter after soaking, wash the fish skin with deionized water, and dry the fish skin after the surface moisture, every 1g Add 10mL of 7g / mL acetic acid solution and sodium alginate accounting for 0.3% of the mass of cod skin to the cod skin to swell for 12 hours, beat the pulp, adjust the pH to 7.5, and obtain the fish skin slurry for later use;

[0024] (2)...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of collagen peptide preparation, and particularly relates to a method for producing collagen peptide by using cod skin as a raw material. The method comprises the following steps: cleaning cod skin, adding a gradient sodium chloride- citric acid aqueous solution, stirring and soaking, dropwise adding a calcium bicarbonate aqueous solution, continuouslysoaking, filtering after soaking, cleaning the cod skin with deionized water, airing the surface moisture of the cod skin, adding an acetic acid solution and sodium alginate, swelling, pulping, adjusting the pH value, obtaining fish skin pulp for later use; and adding papain and squalene into the fish skin pulp, carrying out enzymolysis, carrying out enzyme deactivation after the enzymolysis is finished, centrifuging, taking the supernatant, carrying out ultrafiltration on the supernatant, and carrying out freeze drying. According to the extraction method provided by the invention, the collagen body yield can be increased, the extracted collagen peptide is high in purity, uniform in molecular weight distribution, high in oxidation resistance and free of peculiar smell, the bitter and fishysmell of the collagen peptide prepared from aquatic products is reduced to the maximum extent, and the enzymolysis time is greatly shortened.

Description

technical field [0001] The invention belongs to the technical field of preparation of collagen peptides, and in particular relates to a method for producing collagen peptides from cod skin raw materials. Background technique [0002] Collagen is a structural protein of many connective tissues, and its content reaches more than 30% in mammalian proteins. Collagen peptides are enzymatic products of collagen, which are easily absorbed in the form of small peptides and free amino acids. Animal experiments have confirmed that After ingesting radioactive collagen peptides, radioactivity was detected in tissues such as femur, tibia, joints, and muscles, indicating that to a certain extent, collagen peptides participated in the growth and synthesis of the above tissues, and clinical studies have proved that collagen peptides can promote Bone and joint health, relieve pain caused by exercise, improve sarcopenia, promote wound healing, etc., suitable for sports nutrition food. [000...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/34
CPCC12P21/06C07K14/78
Inventor 李江潘美针张格邱学如赵新辉
Owner 山东恒鑫生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products