Marine product preservative with antibacterial peptide loaded on gliadin nanoparticles and preparation method thereof

A technology of gliadin and nanoparticles, which is applied in the direction of antimicrobial preservation of food ingredients, food preservation, plant protein processing, etc., which can solve environmental pollution, fresh-keeping methods and preservatives that cannot be satisfied by people, improper packaging, etc. question

Pending Publication Date: 2021-03-19
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is a big problem to ensure the value of seafood in the process of preservation and transportation, such as microbial erosion, which leads to corruption of seafood; improper packaging, due to extrusion between products, resulting in mechanical damage, the commodity value and edible value of seafood will be greatly affected
There are also many types of seafood preservatives on the market today, but there are many deficiencies, such as polluting the environment, short preservation time, and high cost.
[0003] Nisin is an antimicrobial peptide composed of 34 amino acids, which is active against many Gram-positive bacteria but has poor stability
Chinese patent CN201310081187.8 discloses "a natural seafood preservative and its preparation method", which discloses a seafood preservation prepared by adding liquids such as glacial acetic acid and water, using Nisin and citrus young fruit extracts as basic raw materials agent, it can all play an effective antibacterial effect, and has a certain fresh-keeping effect to seafood preservation, but the antistaling agent antibacterial time of this invention is not ideal enough
Under such background, existing fresh-keeping methods and preservatives for seafood products obviously cannot be satisfied by people.

Method used

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  • Marine product preservative with antibacterial peptide loaded on gliadin nanoparticles and preparation method thereof
  • Marine product preservative with antibacterial peptide loaded on gliadin nanoparticles and preparation method thereof

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preparation example Construction

[0020] The invention provides a preparation method of a seafood antistaling agent loaded with antimicrobial peptides on gliadin nanoparticles, which comprises the following steps:

[0021] (1) Extraction of gliadin: take 10 parts of wheat area and dissolve in 100mL ethanol solution of 70% (v / v), stir at 35°C for 5h, collect the supernatant after centrifugation for 15 minutes, and extract the obtained The supernatant is subjected to rotary evaporation and freeze-drying to obtain gliadin powder;

[0022] (2) Preparation of gliadin-Nisin nanoparticles: 3-5 parts of gliadin powder obtained in step (1) and 0.05 part of Nisin were dissolved in 70% (v / v) 100mL ethanol solution in 25°C, stirred for 2h, took 20mL of the mixed liquid and added it dropwise to 100mL distilled water, stirred at 25°C for 3h, after rotary evaporation and centrifugation, the obtained gliadin-Nisin nanoparticle solution was placed in Store at 4°C for later use;

[0023] (3) Preparation of ChNW solution: Diss...

Embodiment 1

[0028] A preparation method for seafood preservatives loaded with antimicrobial peptides on gliadin nanoparticles, comprising the following steps:

[0029] (1) Extraction of gliadin: Take 10 portions of wheat area and dissolve them in 70% (v / v) 100mL ethanol solution, stir at 400r / min for 5h at 35°C, and spin in a centrifuge at 3000rpm , centrifuged for 15 min, and collected the supernatant. The solution was put into a rotary evaporator to remove the alcohol in the solution, and evaporated at -0.1Pa for 20min at 55°C. Finally, the sample was frozen at -80°C for 12 hours, and then freeze-dried for 48 hours to obtain gliadin powder;

[0030] (2) Preparation of gliadin-Nisin nanoparticles: 4 parts of gliadin and 0.05 part of Nisin were dissolved in 100 mL of ethanol solution of 70% (v / v), at 25° C., with magnetic stirring Stir at a speed of 400r / min for 2h; take 20mL of the original solution and drop it in 100mL of distilled water at a speed of 8mL / h, and stir for 3h at a speed...

Embodiment 2

[0034] (1) Extraction of gliadin: Take 10 portions of wheat area and dissolve them in 70% (v / v) 100mL ethanol solution, stir at 400r / min for 5h at 35°C, and spin in a centrifuge at 3000rpm , centrifuged for 15 min, and collected the supernatant. The solution was put into a rotary evaporator to remove the alcohol in the solution, and evaporated at -0.1Pa for 20min at 55°C. Finally, the sample was frozen at -80°C for 12 hours, and then freeze-dried for 48 hours to obtain gliadin powder;

[0035] (2) Preparation of gliadin-Nisin nanoparticles: 3 parts of gliadin and 0.05 part of Nisin were dissolved in 100 mL of ethanol solution of 70% (v / v), at 25° C., with magnetic stirring Stir at a speed of 400r / min for 2h; take 20mL of the original solution and drop it in 100mL of distilled water at a speed of 8mL / h, and stir for 3h at a speed of 400r / min at 25°C; put the solution in a rotary evaporator for 55 °C, -0.1Pa conditions, rotary evaporation for 20min to remove the alcohol in the...

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Abstract

The invention provides a preparation method of a marine product preservative with antibacterial peptide (Nisin) loaded on Gliadin Nanoparticles (GNPs). The preparation method comprises the following steps of: mixing extracted gliadin with Nisin to obtain gliadin-Nisin nanoparticles; and mixing the obtained gliadin-Nisin nanoparticle solution with an impurity-removed nano chitin whisker (ChNW) solution to obtain the marine product preservative with antibacterial peptide loaded on gliadin nanoparticles. The marine product preservative is prepared by taking Nisin as a main raw material and otheraids as auxiliary materials, and the stability and bioavailability of Nisin are improved by embedding Nisin with GNPs. Meanwhile, the stability of GNPs is improved by adding ChNW into the GNPs solution. Nisin acts on a cytoplasmic membrane to cause exudation of cytoplasm such as adenosine triphosphate, namely matrix in cytoplasm is damaged to lose activity, so that the bacteriostatic and bactericidal effects are achieved. Experimental results show that the marine product preservative has excellent performances, has indexes exceeding those of commercially available common preservatives, has excellent antibacterial property, is short in biodegradation time, is safe and environment-friendly, and can completely replace the existing common preservatives.

Description

technical field [0001] The invention relates to a seafood preservative and a preparation method thereof, in particular to a seafood preservative in which gliadin nanoparticles are loaded with antibacterial peptides and a preparation method thereof. Background technique [0002] With the improvement of living standards, people have higher and higher requirements for food quality. Seafood produced in coastal cities can already be sold to the whole country, even the whole world. However, it is a big problem to ensure the value of seafood in the process of preservation and transportation, such as microbial erosion, which leads to corruption of seafood; improper packaging, due to extrusion between products, resulting in mechanical damage, the commodity value and edible value of seafood will be greatly affected. There are also many types of fresh-keeping agents for seafood on the market today, but there are many deficiencies, such as polluting the environment, short fresh-keepin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B4/20A23L3/3463A23J1/12A23J3/18
CPCA23B4/20A23L3/3463A23J1/12A23J3/18A23V2002/00A23V2200/10
Inventor 吴春华赵建波姜海鑫孙继帅段孟霞俞珊
Owner FUJIAN AGRI & FORESTRY UNIV
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