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Electrochemical biosensor based on ZnO nano material and method for detecting glucose concentration by using same

A technology of biosensors and nanomaterials, applied in the field of glucose detection and glucose electrochemical biosensors, can solve the problems of harsh photochemical measurement conditions, high environmental conditions, and inability to increase light intensity, etc., achieve good photochemical stability, improve Effects of detection sensitivity and improvement of electron transfer rate

Active Publication Date: 2021-03-19
南通市疾病预防控制中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The measurement conditions of the photochemical method are harsh, time-consuming, and require a large amount of blood; the photoacoustic detection method has high sensitivity but requires high environmental conditions, which affects the measurement results; the measurement disadvantage of the Raman spectroscopy method: because the detection part is the anterior chamber, it cannot be increased. Large light intensity, weak detection signal, and difficult signal processing; while the electrochemical biosensor method has the advantages of high detection accuracy, simple operation, fast analysis speed, small size, easy to carry, and low price.

Method used

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  • Electrochemical biosensor based on ZnO nano material and method for detecting glucose concentration by using same
  • Electrochemical biosensor based on ZnO nano material and method for detecting glucose concentration by using same
  • Electrochemical biosensor based on ZnO nano material and method for detecting glucose concentration by using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) First prepare flower-like ZnO nanomaterials:

[0029] Take 0.2726 g (0.05 mol L −1 ) Zinc dichloride (ZnCl 2 , AR), 4.8048 g (2 mol L −1 ) urea (CON 2 H 4 , AR) and 35 ml of deionized water in a Teflon inner cup, stir for 5 min, adjust the pH to 4 with dilute hydrochloric acid, stir for 10 min, transfer the Teflon inner cup into a stainless steel autoclave and Sealed, placed in an oven and heated at 90 °C for 12 h, the system was naturally cooled to room temperature, and the white precipitate in the reaction system was separated and obtained by centrifugation, washed with absolute ethanol and water for 5 times, and dried in a vacuum drying oven for 60 Dry at °C for 12 h, take out the material and grind it into pellets with a particle size of less than 10-20 nm, and then calcine the ground pellets at 300 °C for 30 min to obtain ZnO nanomaterials; the SEM image of the ZnO nanomaterials is as follows figure 1 As shown in a, its shape is like a flower-like structur...

Embodiment 2

[0037] In this example, the GOx / ZnO / Nafion / GCE sensor and the GOx / Nafion / GCE sensor prepared in the above Example 1 are used to detect the glucose concentration. The specific detection methods are:

[0038] First, a method for glucose concentration detection using GOx / ZnO / Nafion / GCE sensor,

[0039] In the first step, by cyclic voltammetry in the electrochemical workstation, add 10ml of PBS solution with continuous stirring, air saturation, concentration of 0.1 M, pH 7.0 to the detection cell as the test base solution, GOx / ZnO / Nafion / GCE sensor is the working electrode, the saturated calomel electrode is used as the auxiliary electrode, and the platinum sheet electrode is used as the counter electrode, and the glucose solution with a molar volume concentration of 0.1 mol / L is sequentially added to the detection cell according to the volume in Table 1, and the current response value of the working electrode is detected. And output the current response time curve of each quanti...

Embodiment 3

[0049] In this example, according to the electrochemical detection method in the first step of Example 2, the GOx / ZnO / Nafion / GCE sensor is used as the working electrode, and 10 microliters of glucose solution with a concentration of 0.1mol / L is continuously added in parallel in the detection cell to carry out leveling. After 6 times of line detection, the relative standard deviation of the current response value of the working electrode is 3.5%, indicating that the sensor has high measurement accuracy.

[0050] At the same time, take another 3 GOx / ZnO / Nafion / GCE sensors and 3 detection cells with the same conditions. According to the electrochemical detection method in step 1 of Example 2, respectively, add 10 microliters to the concentration of 0.1mol / L The glucose solution was measured repeatedly for 6 times, and the relative standard deviation of the current was 6.5%, indicating that the detection results of the sensor had good reproducibility. After 6 consecutive measureme...

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Abstract

The invention relates to a glucose electrochemical biosensor based on a ZnO nano material and a method for detecting glucose concentration by using same. The glucose electrochemical biosensor is obtained by jointly modifying the surface of a glassy carbon electrode with the ZnO nano material and glucose oxidase; when the glucose electrochemical biosensor is used for electrochemical detection of glucose concentration, the method comprises the following steps: continuously dropwise adding glucose solutions with different concentrations in a detection pool by taking a PBS buffer solution as a base solution and the glucose electrochemical biosensor as a working electrode to obtain a current-time curve; fitting a standard curve of glucose concentration and current under the detection conditions; adding a certain volume of glucose solution to be detected into the detection cell and the same detection cell according to the current-time curve method, and reading a response current A value of the working electrode; and finally, determining the concentration of glucose in the detection pool according to the points drawn on the standard curve, and performing converting to obtain the concentration of the glucose to-be-detected solution in the third step.

Description

technical field [0001] The invention relates to the technical field of electrochemical analysis and detection, in particular to a glucose electrochemical biosensor based on ZnO nanomaterials and a method for glucose detection. Background technique [0002] Diabetes can cause a variety of complications in the body, which is very harmful to the body. At present, there is no cure or drug for diabetes, so the early diagnosis of diabetes and the daily monitoring of human blood sugar are particularly important. By detecting the concentration of glucose in human blood, we can indirectly know people's physical health status, and provide a means for the diagnosis of diabetes. [0003] At present, the methods of detecting glucose concentration include electrochemical sensor method, photochemical method (ie, biochemical analyzer), photoacoustic spectroscopy detection method, Raman spectroscopy detection method, etc. Photochemical measurement conditions are harsh, time-consuming, and r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/48
CPCG01N27/308G01N27/3277G01N27/3278G01N27/48
Inventor 杨娟杨占军陈建忠平文卉顾俊陈峰杨清华戴志英杨梅桂张颖茜施逸岚叶青华
Owner 南通市疾病预防控制中心
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