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Probiotics freeze-dried powder as well as preparation method and application thereof

A technology of probiotic freeze-dried powder and probiotics, applied in biochemical equipment and methods, methods based on microorganisms, bacteria used in food preparation, etc., can solve the problems of low number of viable bacteria and low survival rate, and achieve retention Short time, improved survival rate, quick freezing effect

Active Publication Date: 2021-03-26
瞿瀚鹏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a probiotic freeze-dried powder and its preparation method and application in order to overcome the problems of low viable count, low freezing and shelf-life survival rate in probiotics existing in the prior art

Method used

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  • Probiotics freeze-dried powder as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] (1) Preparation of probiotic fermentation medium: 20 tons of main fermentation tank, feeding volume 80%

[0052] Lactose 0.8%, glucose 4.5%, peptone 0.6%, yeast extract 1.6%, diammonium hydrogen citrate 0.3%, dipotassium hydrogen phosphate 0.25%, magnesium sulfate 0.5%, the rest is tap water, adjust the pH to 6.5, and prepare 121 Sterilize at ℃ for 30 minutes, cool down for later use;

[0053] (2) Under aseptic conditions, insert the bacterial classification of Lactobacillus bulgaricus into the fermentation medium of step (1) according to the inoculum amount of 5%, cultivate 20h under the condition that the temperature is 35°C and the rotating speed is 50rpm to obtain fermentation During the fermentation process, 20% ammonia water was added dropwise to control the pH value of the fermentation medium to be constant at 5.5, and the glucose mass fraction of 17wt% glucose solution was supplemented in batches to keep the reducing sugar concentration in the fermentation solut...

Embodiment 2

[0057] (1) Preparation of probiotic fermentation medium: 20 tons of main fermentation tank, feeding volume 80%

[0058] Lactose 1%, glucose 4.3%, peptone 0.6%, yeast extract 1.8%, diammonium hydrogen citrate 0.3%, dipotassium hydrogen phosphate 0.25%, magnesium sulfate 0.5%, the rest is tap water, adjust the pH to 6.5, and prepare 121 Sterilize at ℃ for 30 minutes, cool down for later use;

[0059] (2) Under sterile conditions, insert the bacterial classification of Bifidobacterium bifidum into the fermentation medium of step (1) according to the inoculum size of 5%, and cultivate it for 24h under the condition that the temperature is 37°C and the rotating speed is 50rpm Obtain fermented liquid, add sodium carbonate solution in the fermented culture process to control the pH value of fermented medium to be constant at 6.0, and supplement the glucose liquid that the mass fraction of glucose is 17wt% to keep reducing sugar concentration in the fermented liquid to be 0.6-2wt% in ...

Embodiment 3

[0063] (1) Preparation of probiotic fermentation medium: 20 tons of main fermentation tank, feeding volume 80%

[0064] Lactose 1%, glucose 4.5%, peptone 0.7%, yeast extract 2%, diammonium hydrogen citrate 0.3%, dipotassium hydrogen phosphate 0.25%, magnesium sulfate 0.5%, the rest is tap water, adjust the pH to 6.5, and prepare 121 Sterilize at ℃ for 30 minutes, cool down for later use;

[0065] (2) Under sterile conditions, insert the strain of Lactobacillus bulgaricus into the fermentation medium of step (1) according to the inoculum size of 5%, and cultivate it for 28h under the condition that the temperature is 37°C and the rotation speed is 50rpm to obtain fermentation During the fermentation process, the sodium hydroxide solution with a mass fraction of 3wt% was added to control the pH value of the fermentation medium at 6.5, and the glucose solution with a mass fraction of 17wt% was supplemented in batches to maintain the reducing sugar concentration in the fermentatio...

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Abstract

The invention relates to a probiotic production process, and discloses probiotic freeze-dried powder as well as a preparation method and application thereof. The preparation method of the probiotic freeze-dried powder comprises the following steps of (1) providing a mixture of probiotic-containing fermented bacterial sludge, a protective agent and a moisture activity regulator; and (2) spraying arefrigerating medium to the mixture to freeze the mixture, and carrying out vacuum drying. According to the preparation method, a mode of spraying the refrigerating medium to the probiotic bacterial sludge is adopted so that probiotic bacterial cells can be quickly frozen, the inactivation of protein and enzyme is reduced, ice crystals generated in the freezing process of probiotics are fine, thedamage to cell structures is avoided, and the survival rate of the probiotics in the freezing process is increased.

Description

technical field [0001] The invention relates to probiotic production technology, in particular to a probiotic freeze-dried powder and its preparation method and application. Background technique [0002] With the continuous deepening of research on the mechanism of beneficial effects of probiotics on human health and the enhancement of people's awareness of health care, probiotics have received more and more attention and research and development, and have been widely used in nutrition, health care, food, medicine and other industries. In the food industry, probiotics refer to a type of active microorganisms that are beneficial to the host by colonizing in the human body and changing the composition of the flora in a certain part of the host. The common product forms are "solid beverage", "bacteria powder", "microbial Encapsulated bacterial powder". [0003] In order to facilitate the application of probiotics in the production of nutrition, health care, food, medicine and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A23L33/135A23C9/127A23C9/16A23L2/39A23G3/36A23C9/152A23L2/52C12R1/225C12R1/46C12R1/245C12R1/23C12R1/25C12R1/01
CPCC12N1/20C12N1/04A23L33/135A23C9/127A23C9/16A23L2/39A23G3/366A23C9/152A23L2/52A23V2400/147A23V2400/165A23V2400/173A23V2400/181A23V2400/125A23V2400/123A23V2400/113A23V2400/175A23V2400/513A23V2400/517A23V2400/519A23V2400/529A23V2400/531A23V2400/533A23V2400/249A23V2400/169Y02A50/30
Inventor 梁云曹晟王身健
Owner 瞿瀚鹏
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