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Preparation method and application of nucleic acid extraction magnetic beads

A magnetic bead, nucleic acid technology, applied in DNA preparation, chemical instruments and methods, alkali metal oxides/hydroxides, etc., can solve the problems of easy agglomeration of magnetic nanoparticles, low chemical stability, insufficient surface hydroxyl groups, etc. Achieve the effect of improving biological activity, rapid and thorough solid-liquid separation, and easy operation

Inactive Publication Date: 2021-04-02
陈美玲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the small size effect of nano-Fe3O4, magnetic dipole attraction, etc., magnetic nanoparticles are easy to agglomerate, and the chemical stability is not high, easy to be oxidized, and the surface hydroxyl group is insufficient, making it difficult to directly apply to the biological field.

Method used

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  • Preparation method and application of nucleic acid extraction magnetic beads
  • Preparation method and application of nucleic acid extraction magnetic beads

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A preparation method for nucleic acid extraction magnetic beads, comprising the following steps:

[0034] Step 1, nano-magnetic oxide MnFe 2 o 4 Preparation: In a reaction flask, add (2mmol, 0.541g) iron trichloride hexahydrate, 1mmol manganese chloride tetrahydrate and 16mL ethylene glycol, stir vigorously to dissolve, add 2.16g of sodium trifluoroacetate With 1.08g of poly(4-styrenesulfonic acid-copolymerization-maleic acid) sodium salt, it was completely dissolved to form a homogeneous mixture. Under the condition of vigorous stirring, 2.16mL of ammonia water was slowly added dropwise until precipitation occurred, and then Sonicate for 30 minutes, put the reaction bottle into a high-temperature reaction furnace, raise the temperature to 190° C., and react for 6 hours. After the reaction is over, use a stronger magnetic magnet to obtain nano-magnetic oxide MnFe through magnetic separation. 2 o 4 , ultrasonically cleaned three times with deionized water and ethanol...

Embodiment 2

[0037] A preparation method for nucleic acid extraction magnetic beads, comprising the following steps:

[0038] Step 1, nano-magnetic oxide MnFe 2 o 4Preparation: In a reaction flask, add (2.5mmol, 0.675g) iron trichloride hexahydrate, 1mmol manganese chloride tetrahydrate and 22mL ethylene glycol, stir vigorously to dissolve, add 3.38g of trifluoroacetic acid Sodium and 1.35g of poly(4-styrenesulfonic acid-copolymerization-maleic acid) sodium salt were dissolved completely to form a homogeneous mixture. Under vigorous stirring conditions, 3.0mL ammonia water was slowly added dropwise until precipitation occurred. After ultrasonication for 30 minutes, the reaction bottle was put into a high-temperature reaction furnace, and the temperature was raised to 190° C. for 6 hours of reaction. After the reaction is over, use a stronger magnetic magnet to obtain nano-magnetic oxide MnFe through magnetic separation. 2 o 4 , ultrasonically cleaned three times with deionized water an...

Embodiment 3

[0041] A preparation method for nucleic acid extraction magnetic beads, comprising the following steps:

[0042] Step 1, nano-magnetic oxide MnFe 2 o 4 Preparation: In a reaction flask, add (3mmol, 0.811g) iron trichloride hexahydrate, 1mmol manganese chloride tetrahydrate and 28mL ethylene glycol, stir vigorously to dissolve, add 4.87g of sodium trifluoroacetate With 1.62g of poly(4-styrenesulfonic acid-copolymerization-maleic acid) sodium salt, dissolve it completely to form a homogeneous mixture, and slowly add 4.0mL of ammonia water dropwise under vigorous stirring until precipitation occurs, then Sonicate for 30 minutes, put the reaction bottle into a high-temperature reaction furnace, raise the temperature to 190° C., and react for 6 hours. After the reaction is over, use a stronger magnetic magnet to obtain nano-magnetic oxide MnFe through magnetic separation. 2 o 4 , ultrasonically cleaned three times with deionized water and ethanol to obtain a pure product, and f...

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Abstract

The invention discloses a preparation method of nucleic acid extraction magnetic beads. The preparation method comprises the steps of 1, preparing a nano magnetic oxide MnFe2O4; and 2, preparing nucleic acid extraction magnetic beads. According to the preparation method of the nucleic acid extraction magnetic beads, the nano magnetic oxide MnFe2O4 is prepared by adopting a chemical coprecipitationmethod, the nucleic acid extraction magnetic beads are prepared by adopting an ultrasonic-assisted sol-gel method, and the preparation method is simple in process, simple and convenient to operate and suitable for batch production.

Description

technical field [0001] The invention belongs to the technical field of magnetic material preparation, and in particular relates to a preparation method and application of nucleic acid extraction magnetic beads. Background technique [0002] Magnetic beads are a functionalized carrier coated with biologically active groups, which can be dispersed in the base liquid to form magnetic liquid materials. The biologically active groups can be coupled with various biologically active substances, and have the fluidity of liquid And the dual characteristics of solid magnetic granular materials. The magnetic beads can move and concentrate in a direction under the action of an external magnetic field, and can restore the original structure and state after the external magnetic field is removed, thus making the complex liquid-solid separation technology quick and easy. Targeted substances with high purity can be obtained by simple elution. [0003] At present, magnetic beads have been ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/06B01J20/28B01J20/30C12N15/10
CPCB01J20/06B01J20/28009C12N15/1013
Inventor 陈美玲张晓蕾
Owner 陈美玲
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