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Preparation method and application of photo-thermal driven drug release hydrogel microspheres

A hydrogel microsphere, hydrogel technology, applied in biochemical equipment and methods, gel preparation, animal cells, etc., can solve the lack of environmental responsive regulation, amplification efficiency to be improved, immune effector cytotoxicity, etc. problem, to achieve the effect of in vitro proliferation, controllable release, and good biocompatibility

Active Publication Date: 2021-04-02
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE +1
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current systems for expanding immune effector cells in vitro are often nano-scaffold materials, which are prone to toxicity to immune effector cells, and their expansion efficiency needs to be improved.
Secondly, the current T cell expansion system also lacks environmental responsive regulation and is not intelligent enough.

Method used

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  • Preparation method and application of photo-thermal driven drug release hydrogel microspheres
  • Preparation method and application of photo-thermal driven drug release hydrogel microspheres
  • Preparation method and application of photo-thermal driven drug release hydrogel microspheres

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preparation example Construction

[0024] The invention provides a method for preparing photothermally driven drug release hydrogel microspheres, comprising the following steps:

[0025] S1. Preparation of silica photonic crystal microspheres:

[0026] Mix silicon dioxide particles with water to uniformly disperse silicon dioxide in water. Using a microfluidic chip, use the above-mentioned silicon dioxide dispersion as the internal phase and silicone oil as the external phase to prepare silicon dioxide droplets, and pass the peristaltic The pump controls the flow rate of the inner and outer phases to control the size of the silica droplets; the droplet template is heated and dried to completely evaporate the water in the silica droplets, and the silicone oil is washed away, followed by high-temperature calcination to obtain silica photonic crystal microspheres ;

[0027] S2. Drug loading in silica photonic crystal microspheres

[0028] Soak the silica photonic crystal microspheres prepared in S1 in the hydrog...

Embodiment 1

[0030] Example 1——Preparation of photothermally driven drug release hydrogel microspheres

[0031] S1. Preparation of silica photonic crystal microspheres:

[0032] Mix silicon dioxide particles with water to uniformly disperse silicon dioxide in water. Using a microfluidic chip, use the above-mentioned silicon dioxide dispersion as the internal phase and silicone oil as the external phase to prepare silicon dioxide droplets, and pass the peristaltic The pump controls the flow rate of the inner and outer phases to control the size of the silica droplets; the container that has collected the droplet stencil is placed in an oven at 75°C for 12 hours, and the droplets gradually change from milky white to transparent; to ensure that the moisture in the droplets is completely Evaporate, raise the temperature of the oven to 95°C and keep it for 2 hours; take out the collection vessel from the oven, recover the silicone oil in the upper layer after cooling, then rinse slowly with n-h...

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Abstract

The invention provides a preparation method and application of photo-thermal driven drug release hydrogel microspheres. The method is based on photonic crystal microspheres, and photonic crystal hydrogel hybrid microspheres with internal drug loading are prepared by means of microfluidic chip preparation, hydrogel filling and the like. The prepared photo-thermal driven drug release hydrogel microspheres can respond to irradiation of near-infrared light, controllably release carried active antibodies and cell growth factors, promote activation of T cells in vitro and realize in-vitro amplification of the T cells. The method is reasonable in design, simple to operate, low in preparation cost, capable of being widely applied to T cell in-vitro activation and amplification, high in practicability and suitable for popularization.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, in particular to a preparation method and application of photothermally driven drug release hydrogel microspheres. Background technique [0002] Tumor immunotherapy is an emerging tumor treatment plan. It is a powerful tumor-killing treatment that enhances the anti-tumor ability of tumor patients by activating their own immune system. It has the advantages of small side effects and wide indications. However, the immunosuppressive microenvironment inside the tumor always weakens the killing process of T cells to the tumor. Clinically, this problem is mainly solved by infusing in vivo activated immune effector cells into patients, a treatment method also known as adoptive cell therapy (ACT). The number of immune effector cells required for clinical treatment is extremely large, and a very effective in vitro expansion system is required to prepare a large number of immune cells. [0003] The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783B01J13/00
CPCC12N5/0636B01J13/0056B01J13/0065C12N2501/2302C12N2501/2315C12N2501/2321C12N2501/515C12N2501/51
Inventor 沈贤寿鑫赵远锦孙维健王月桐商珞然
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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