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Magnetic cell and preparation method and application thereof

A cell and magnetic technology, applied in the field of bioremediation, can solve the problems of poor dispersion performance, difficult recovery, low mechanical strength, etc., and achieve the effect of good free dispersion.

Pending Publication Date: 2021-04-02
自然资源部天津海水淡化与综合利用研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is not suitable for in-situ treatment of oil pollution in a natural environment, and the immobilization cost and reaction cost are high
[0005] The above existing technologies all reflect the high efficiency and reliability of immobilized microorganisms in degrading oil pollution. However, the immobilization of bacteria mostly adopts the method of carrier adsorption or carrier embedding, which has low mechanical strength, poor mass transfer performance, and poor dispersion performance. , difficult to recycle and other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] In this embodiment, the preparation of magnetic cells with the function of degrading oil stains includes the following steps:

[0065] (1) Collect the oily sludge from Dagang Oilfield in Tianjin, add sterile physiological saline to immerse the sludge and fully mix it, leave it to wait for the sediment to settle, and take the supernatant as the bacterial suspension to be screened;

[0066] The bacterium suspension to be screened is inoculated in oil-containing liquid medium (2.5g / L NaNO 3 , 0.5g / LK 2 HPO 4 , 1g / L KH 2 PO 4 , 0.01g / L CaCl 2 , 0.1g / L KCl, 0.5g / L MgSO 4 ·7H 2 O, 0.01g / L FeSO 4 ·7H 2 O, 0.1g / L yeast powder, 0.5mL / L trace element solution and 1% crude oil, wherein the trace element solution includes 0.26g / LH 3 BO 3 , 0.5g / L CuSO 4 ·5H 2 O, 0.5g / L MnSO 4 ·H 2 O, 0.06g / L MoNa 2 o 4 2H 2 O and 0.7g / L ZnSO 4 ·7H 2 (0), 30°C, 160r / min vibrating shaker culture for 7 days, select the experimental group whose surface oil pollution is obviously redu...

Embodiment 2

[0071] In this embodiment, the preparation of magnetic cells with the function of degrading oil stains includes the following steps:

[0072] (1) Collect the oily sludge from Dagang Oilfield in Tianjin, add sterile physiological saline to immerse the sludge and fully mix it, leave it to wait for the sediment to settle, and take the supernatant as the bacterial suspension to be screened;

[0073] The bacteria suspension to be screened was inoculated in oily liquid medium (2.5g / L NaNO 3 , 0.5g / LK 2 HPO 4 , 1g / L KH 2 PO 4 , 0.01g / L CaCl 2 , 0.1g / L KCl, 0.5g / L MgSO 4 ·7H 2 O, 0.01g / L FeSO 4 ·7H 2 O, 0.1g / L yeast powder, 0.5mL / L trace element solution and 1% crude oil, wherein the trace element solution includes 0.26g / LH 3 BO 3 , 0.5g / L CuSO 4 ·5H 2 O, 0.5g / L MnSO 4 ·H 2 O, 0.06g / L MoNa 2o 4 2H 2 O and 0.7g / L ZnSO 4 ·7H 2 (0) in 28°C, 150r / min shaking shaker culture for 7 days, select the experimental group whose surface oil pollution is obviously reduced, and th...

Embodiment 3

[0078] In this embodiment, the preparation of magnetic cells with the function of degrading oil stains includes the following steps:

[0079] (1) Collect the oily sludge from Dagang Oilfield in Tianjin, add sterile physiological saline to immerse the sludge and fully mix it, leave it to wait for the sediment to settle, and take the supernatant as the bacterial suspension to be screened;

[0080] The bacterium suspension to be screened is inoculated in oil-containing liquid medium (2.5g / L NaNO 3 , 0.5g / LK 2 HPO 4 , 1g / L KH 2 PO 4 , 0.01g / L CaCl 2 , 0.1g / L KCl, 0.5g / L MgSO 4 ·7H 2 O, 0.01g / L FeSO 4 ·7H 2 O, 0.1g / L yeast powder, 0.5mL / L trace element solution and 1% crude oil, wherein the trace element solution includes 0.26g / LH 3 BO 3 , 0.5g / L CuSO 4 ·5H 2 O, 0.5g / L MnSO 4 ·H 2 O, 0.06g / L MoNa 2 o 4 2H 2 O and 0.7g / L ZnSO 4 ·7H 2 (0) in 28°C, 150r / min shaking shaker culture for 10 days, select the experimental group whose surface oil pollution is obviously red...

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Abstract

The invention provides a magnetic cell and a preparation method and application thereof. The magnetic cell is an oil stain degrading bacterium adsorbed with magnetic nanoparticles; and the preparationmethod of the magnetic cell comprises the following steps: co-incubating the oil stain degrading bacteria and the magnetic nanoparticles, and culturing and activating in an activation culture mediumto obtain the magnetic cell. The magnetic cells are good in dispersity, good in mass transfer property, easy to recover, high in oil stain degradation capacity, simple in preparation method and mild in condition, and have important application prospects in the field of bioremediation of petroleum and petroleum product polluted water environments.

Description

technical field [0001] The invention belongs to the technical field of bioremediation of water environment polluted by petroleum and petroleum products, and relates to a magnetic cell and its preparation method and application. Background technique [0002] With the increasing intensity of exploration and exploitation of petroleum resources, the crude oil industry develops rapidly. While crude oil and its products bring economic benefits to the society, they also cause great damage to the natural environment on which human beings depend. Crude oil pollutants pass through Various ways into the soil, surface water and groundwater, seriously polluting the environment. After an oil pollution accident, physical and chemical methods are usually used to clean up most of the surface oil. However, the alkane and aromatic hydrocarbon pollutants in the residual oil still exist in the water body, and their harm should not be underestimated. It may be harmful to the water body and human ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N1/20C12N1/02C01G49/08C02F3/34B82Y30/00C12R1/01C02F101/32
CPCC12N11/14C12N1/20C12N1/02C01G49/08C02F3/34B82Y30/00C01P2006/42C02F2101/32
Inventor 马晓蕾曹军瑞王旭楠谢宝龙王勋亮
Owner 自然资源部天津海水淡化与综合利用研究所
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