Ultrasonic-assisted mineral chelated almond peptide as well as preparation method and application thereof

An ultrasound-assisted, mineral technology, applied in the field of medicine, can solve the problem of no mineral chelated almond peptide, etc., to achieve the effect of improving the metal chelation rate, facilitating industrial production, and shortening the reaction time

Active Publication Date: 2021-04-06
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no products and processes for mineral chelated almond peptides...

Method used

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  • Ultrasonic-assisted mineral chelated almond peptide as well as preparation method and application thereof
  • Ultrasonic-assisted mineral chelated almond peptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Mix 100g of 80-mesh almond powder with 1000mL of n-hexane, react at 50°C for 1h and filter, then mix 50g of filter residue with 500mL of deionized water, adjust the pH to 8.0 with 1mol / L NaOH aqueous solution, and centrifuge at 3000rpm for 30min to obtain the above supernatant: adjust the pH of the supernatant to 4.5 with 1 mol / L HCl, centrifuge at 5000 rpm for 30 min to obtain a precipitate, and freeze-dry to obtain almond protein.

[0042](2) Add 50g of almond protein to 500mL of pH=7 phosphate buffer solution, add 0.5g of alkaline protease, and turn on the ultrasound at the same time, with a frequency of 30KHz and a power of 500W for 10min. Centrifuge for 30 minutes to obtain the supernatant, and freeze-dry to obtain almond polypeptide. The average molecular weight of the almond polypeptide in this example is 310Da.

[0043] (3) Dissolve 10 g of almond polypeptide into 100 mL of deionized water to obtain an aqueous solution of almond polypeptide; dissolve ferrou...

Embodiment 2

[0045] (1) Mix 100g of 200-mesh almond powder with 500mL of n-hexane, react at 20°C for 2h and filter, then mix 50g of filter residue with 1000mL of deionized water, adjust the pH to 9.0 with 3mol / L NaOH aqueous solution, and centrifuge at 6000rpm for 10min to obtain the above supernatant: adjust the pH of the supernatant to 6.5 with 3 mol / L HCl, centrifuge at 10,000 rpm for 10 min to obtain a precipitate, and freeze-dry to obtain almond protein.

[0046] (2) Add 1000mL of pH=8 phosphate buffer solution to 50g of almond protein, add 0.5g of flavor protease and 0.5g of neutral protease, and turn on the ultrasound at the same time, with a frequency of 80KHz and a power of 50W for 30min. After the ultrasound is finished, boil for 20min and cool down. After reaching room temperature, centrifuge at 10,000 rpm for 10 minutes to obtain supernatant, and freeze-dry to obtain almond polypeptide. The average molecular weight of almond polypeptide in this example is 580Da.

[0047] (3) D...

Embodiment 3

[0049] (1) Mix 100g of 100-mesh almond powder with 800mL of n-hexane, react at 30°C for 1.5h and then filter, then mix 50g of filter residue with 800mL of deionized water, adjust the pH to 8.5 with 2mol / L NaOH aqueous solution, and centrifuge at 4000rpm for 20min to obtain Supernatant: adjust the pH of the supernatant to 5 with 2 mol / L HCl, centrifuge at 8000 rpm for 20 min to obtain a precipitate, and freeze-dry to obtain almond protein.

[0050] (2) Add 800mL of phosphate buffer solution with pH=7.5 to 50g of almond protein, add 0.5g of trypsin and 0.5g of papain, and turn on the ultrasound at the same time, with a frequency of 50KHz and a power of 200W for 20min. After the ultrasound is finished, boil for 18min and cool After reaching room temperature, centrifuge at 8000rpm for 20min to obtain supernatant, and freeze-dry to obtain almond polypeptide. The average molecular weight of the almond polypeptide in this example is 400Da.

[0051] (3) Dissolve 10 g of almond polype...

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Abstract

The invention belongs to the technical field of medicines, and discloses an ultrasonic-assisted mineral chelated almond peptide as well as a preparation method and application thereof. The method comprises the following steps of 1) in a phosphoric acid buffer solution, carrying out enzymolysis on almond protein by adopting protease under an ultrasonic condition, deactivating enzyme, centrifuging, taking supernatant, and carrying out freeze drying to obtain almond polypeptide, 2) in a solvent, chelating and centrifuging the almond polypeptide and minerals under an ultrasonic condition, taking supernatant, and freeze-drying to obtain ultrasonic-assisted mineral chelated almond peptide. The minerals are water-soluble ferrite, water-soluble calcium salt and water-soluble zinc salt; and the solvent is water and/or a phosphate buffer. The chelated almond peptide containing multiple minerals is obtained through ultrasonic assistance and chelation of different minerals, the yield is high, the oxidation resistance is good, and meanwhile multiple mineral nutrients are provided. The method provided by the invention is simple. The prepared ultrasonic-assisted mineral chelated almond peptide is used as an antioxidant peptide, and is used in the fields of food, health products and/or medicines.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to an ultrasonic-assisted mineral chelation almond peptide, a preparation method thereof and an application as an antioxidant peptide. Background technique [0002] Minerals play a variety of biological functions on human health. For example, calcium, as the most abundant inorganic element in the body, is considered to be one of the essential nutritional minerals for the human body. , muscle contraction and heart function all have important physiological effects. Insufficient calcium intake can lead to metabolic bone diseases such as rickets in children and osteoporosis in the elderly. Zinc acts as a catalytic component of a series of enzymes and plays structural and biological roles in many proteins, peptides, hormones, cytokines, transcription factors and growth factors. Iron is essential for oxygen transport in hemoglobin and is an important raw material for hemoglobin produ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/107A61K38/01A61P39/06A23L33/18A23L33/165A61K33/26A61K33/30A61K33/06
CPCC12P21/06C07K1/1077A61K38/011A61K33/26A61K33/30A61K33/06A61P39/06A23L33/18A23L33/165A23V2002/00A61K2300/00A23V2250/55A23V2250/1592A23V2250/1578A23V2250/1642A23V2200/30Y02A40/90
Inventor 叶勇黄传庆
Owner SOUTH CHINA UNIV OF TECH
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