Cosmetic composition containing galactomyces-derived exosome as active ingredient

A cosmetic composition, galactose technology, applied in the direction of medical preparations, cosmetics, cosmetic preparations containing active ingredients, etc., can solve problems such as foreign body sensation, skin problems, skin redness or dermatitis

Active Publication Date: 2021-04-09
韩国外泌体生技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, functional cosmetics containing plant extracts as active ingredients have problems that they may cause foreign body sensation during their evaporation after application to the skin, and their action duration is short
However, galactomyces ferment filtrate can cause side effects such as skin problems, skin redness, or dermatitis in people whose skin is sensitive to fermented ingredients

Method used

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  • Cosmetic composition containing galactomyces-derived exosome as active ingredient
  • Cosmetic composition containing galactomyces-derived exosome as active ingredient
  • Cosmetic composition containing galactomyces-derived exosome as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Preparation of galactosomal exosomes

[0045] Commercially available galactosaccharomyces strains G. candidum and G. geotrichum used for brewing or fermentation were cultured with shaking, and the conditioned medium was subsequently pelleted by centrifugation and precipitated galactose was removed yeast cells.

[0046] The supernatant of the galactomyces conditioned medium obtained by the above procedure was filtered through a 0.22-μm filter to remove impurities such as cell debris, waste, and large particles. Galactomyces-derived exosomes were isolated from filtered Galactomyces conditioned medium by a tangential flow filtration (TFF) method.

[0047] The size of isolated Galactomyces-derived exosomes was analyzed by transmission electron microscopy (TEM). As shown in Figures 1A and 1B, it was determined that the isolated Galactomyces-derived exosomes were nano-sized vesicles. The size and concentration of Galactomyces-derived exosomes were analyzed by na...

Embodiment 2

[0048] Example 2: Evaluation of the delivery ability of Galactomyces-derived exosomes to dermal fibroblasts

[0049] In order to examine whether galactomyces-derived exosomes would be delivered to human dermal fibroblasts (purchased from CEFO Co., Ltd.), the following analysis was performed. To fluorescently stain the membranes of the galactomyces-derived exosomes prepared in Example 1, the exosomes were reacted with PKH67 fluorescent dye (purchased from Sigma-Aldrich). After the reaction, the reaction solution was fractionated with a MW3000 column (purchased from ThermoFisher Scientific) to remove unstained free PHK67 in the exosome membrane. A negative control was prepared by reacting PKH67 fluorescent dye with buffer solution and fractionating the reaction product with a MW3000 column. Exosomes stained with PKH67 were incubated with pre-cultured human dermal fibroblasts, and fluorescence microscopy was then used to observe whether exosomes would be delivered into the cells...

Embodiment 3

[0050] Example 3: Evaluation of the effect of stimulating collagen production

[0051] Human dermal fibroblasts (purchased from CEFO Co., Ltd.) dispersed in DMEM medium containing fetal bovine serum were dispensed into multiwell plates, and then cultured for 24 hours, and cultured in serum-free medium for another 24 hours. Hour. Thereafter, the galactomyces-derived exosomes prepared in Example 1 were diluted in different concentrations of serum-free medium, and then human dermal fibroblasts were treated with each dilution and cultured. Thereafter, the medium was collected and centrifuged, and then the centrifuged medium was prepared. The amount of collagen synthesized by human dermal fibroblasts and accumulated in the medium was measured using an EIA kit for type I procollagen C-peptide (PIP) (purchased from Takara Bio). The total protein amount of cell lysates was measured with BCA protein assay kit (purchased from ThermoFisher Scientific). Normalize the amount of collagen...

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PUM

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Abstract

The present invention provides a cosmetic composition for skin regeneration, skin elasticity improvement and skin wrinkle reduction, containing a Galactomyces-derived exosome as an active ingredient. The cosmetic composition containing a Galactomyces-derived exosome as an active ingredient, of the present invention, has a functional activity that is useful in functional cosmetics, that is, a much higher increase in collagen synthesis than a filtered product of Galactomyces conditioned media. Therefore, the cosmetic composition of the present invention has excellent effects such as skin regeneration, skin elasticity improvement and / or skin wrinkle reduction.

Description

technical field [0001] The present invention relates to a cosmetic composition for skin regeneration, improvement of skin elasticity and reduction of skin wrinkles, comprising exosomes derived from Galactomyces as an active ingredient. Background technique [0002] Skin aging is known to result in decreased skin elasticity and increased skin wrinkles, and decreased skin elasticity and skin wrinkle formation are known to occur due to decreased collagen synthesis and stimulated expression of collagenase matrix metalloproteinase (MMP). [0003] In addition, it is known that in skin cells, the enzyme COX-2, which produces inflammatory cytokines, increases due to aging or ultraviolet (UV) rays, leading to increased synthesis of prostaglandin E2 and increased production of inflammation inducers. Due to the inflammatory response, the biosynthesis of MMP is increased, leading to the degradation of collagen and leading to the decrease of skin elasticity and the formation of skin wrin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9728A61Q19/00A61Q19/08
CPCA61Q19/08A61K8/14A61K2800/85A61K8/9728A61K36/062A61P17/00A61K8/99A61Q19/00A61K8/0212A61Q19/02
Inventor 李龙源赵柄成
Owner 韩国外泌体生技有限公司
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