Application of erythritol in preparation of medicine for treating non-alcoholic fatty liver
An erythritol, non-alcoholic technology, applied in the field of application of erythritol in the preparation of drugs for the treatment of non-alcoholic fatty liver, can solve the problems of no research and reports on the therapeutic effect
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Embodiment 1
[0032] Example 1 AML12 Cell Oil Red O Staining Experiment
[0033] experimental method:
[0034] (1) AML12 cells were cultured and seeded in six-well plates. The formula of cell culture medium is F12 medium with 4.5g / L glucose, 10% fetal calf serum, 1% penicillin and streptomycin, 1% ITS, 40ng / ml dexamethasone (Sigma, D4902-100mg). Cells were cultured in 5% CO 2 and in a cell culture incubator at 37°C for 12 hours.
[0035] (2) Remove the original culture medium and replace it with F12 with only 4.5g / L glucose as the culture medium to culture the cells, and starve the AML12 cells for 3 hours.
[0036] (3) AML12 cells were co-incubated with 250 mg / ml erythritol and 1000 μM free fatty acid (oleic acid:palmitic acid=2:1) for 24 hours; among them, they were divided into blank group (F12 medium), model group (1000 μM free fatty acid ), control group (250mg / ml erythritol), experimental group (250mg / ml erythritol+1000μM free fatty acid).
[0037] (4) The cell culture medium wa...
experiment example 2W
[0044] Experimental example 2Western blot detection of lipid regulatory protein expression in HEPG2 cells
[0045] experimental method
[0046] (1) Culture HEPG2 cells and inoculate them in a six-well plate with a density of 1×10 per well 6 cells. The formula of the cell culture medium is DMEM medium with 4.5g / L glucose, 10% fetal bovine serum and 1% penicillin and streptomycin. Cells were cultured in 5% CO 2 and in a cell culture incubator at 37°C for 12 hours.
[0047] (2) Remove the original culture medium and replace it with DMEM with only 4.5g / L glucose as the culture medium to culture the cells, and starve the HEPG2 cells for 3 hours.
[0048] (3) Co-incubate HEPG2 cells with 250 mg / ml erythritol and 1000 μM free fatty acid (oleic acid:palmitic acid=2:1) for 24 hours; wherein, they are divided into blank group (DMEM medium), model group (1000 μM free fatty acid ), control group (250mg / ml erythritol), experimental group (250mg / ml erythritol+1000μM free fatty acid)....
Embodiment 3
[0058] Determination of TG in the mouse liver of embodiment 3
[0059] experimental method:
[0060] (1) Raise the mice in an SPF environment with a temperature of 24±1° C. and a relative humidity of 40%-80%.
[0061] (2) Before the experiment, the mice were separated into cages and fasted for 12 hours, and then injected intraperitoneally with 50 mg / kg, 100 mg / kg, and 200 mg / kg of erythritol for 1 hour and then injected with 500 mg / kg of tyloxapol Mice were injected intraperitoneally for 12 hours. The mice were randomly divided into seven groups: blank group (normal saline), model group (500mg / kg tyloxapol), control group (200mg / kg erythritol), drug low dose group (50mg / kg erythritol sugar alcohol+500mg / kg tyloxapol), drug middle dose group (100mg / kg erythritol+500mg / kg tyloxapol), drug high dose group (200mg / kg erythritol+500mg / kg Tyloxapol), drug positive control group (100mg / kg fenofibrate+500mg / kg Tyloxapol).
[0062] (3) The mouse liver was completely removed and cut ...
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