Application of flammulina velutipes extract in gene expression quantity regulation, skin care, depression resistance and cerebrovascular health care

A technology of golden needle flower and extract, applied in golden needle flower extract, anti-depression and cerebrovascular health care, regulation of gene expression, skin care field, can solve problems such as harmfulness to human health and high price

Inactive Publication Date: 2021-04-20
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] However, most of the medicines, food products or skin care products currently used to solve the above problems are made of chemical ingredients. Long-term use is not only harmful to human health, but also these products are often expensive and cannot be afforded by ordinary users

Method used

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  • Application of flammulina velutipes extract in gene expression quantity regulation, skin care, depression resistance and cerebrovascular health care
  • Application of flammulina velutipes extract in gene expression quantity regulation, skin care, depression resistance and cerebrovascular health care
  • Application of flammulina velutipes extract in gene expression quantity regulation, skin care, depression resistance and cerebrovascular health care

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1. Preparation of Golden Needle Flower Extract

[0062] Firstly, the daylilies (planted by farmers in Yuli, Hualien) are homogenized from dry materials, and then at 50-100°C, the homogenized daylilies are treated with an extraction solvent at a volume ratio of 5-20:1-5 The extraction is carried out for 0.5-3 hours to obtain a crude extract, wherein the extraction solvent is water, alcohols, aqueous alcohols or a combination thereof, and the preferred extraction solvent is water. Then, cool to room temperature, and then filter the crude extract with a 400-mesh filter to obtain a filtrate, and then concentrate the filtrate under reduced pressure at 45-70° C. to obtain a daylily flower extract.

Embodiment 2

[0063] Example 2. Efficacy Evaluation of Golden Needle Flower Extract on Anti-Glycation

[0064] This example is to test the anti-glycation activity of the golden needle flower extract of the present invention. Therefore, the saccharification activity is quantified by inhibiting the efficiency of D-fructose from saccharifying collagen (Collagen). First, take 0.2mL of water respectively as a control group, or take 0.2mL of the golden needle flower extract of the present invention with a concentration of 100mg / mL as an experimental group, add 0.2mL of NaN containing 0.06% 3 60mg / mL collagen solution (prepared with 200mM sodium phosphate buffer, pH 7.4), and mixed evenly with 0.2mL of 1.5M D-fructose (prepared with 200mM sodium phosphate buffer, pH 7.4), and took out 0.1 mL of the mixed solution was used as the origin product, and the remaining mixed solution was reacted at 50°C for 24 hours, and then 0.1 mL was taken out as the end product, and then the fluorescence values ​​of ...

Embodiment 3

[0067] Example 3. Efficacy Evaluation of Golden Needle Flower Extract in Inhibiting Fat Accumulation

[0068] In this embodiment, mouse bone marrow stromal cells (bone marrow stromal cells) are used to test the efficacy of the extract of Daylily Flowers of the present invention in inhibiting fat accumulation. The mouse bone marrow stromal cells were purchased from the American Type Culture Collection (USA ), No. CRL-2749 TM. The cells were cultured in pre-adipocyte expansion medium (Pre-adipocyte Expansion Medium) before differentiation, which contained 90% minimum essential medium Alpha medium (minimum essential medium alpha medium, purchased from Gibco, the United States, 12100- 046), 20% fetal bovine serum (Fetal Bovine Serum, purchased from Gibco, the U.S.), and adding 1% penicillin / streptomycin (Penicillin-streptomycin, purchased from Gibco, the U.S.); Medium) to differentiate mouse bone marrow stromal cells containing 90% minimal essential medium Alpha medium, 20% fe...

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Abstract

The invention relates to the field of plant extracts, in particular to application of a flammulina velutipes extract in gene expression quantity regulation, skin care, depression resistance and cerebrovascular health care. The flammulina velutipes extract provided by the invention is used for regulating the expression quantities of an XRCC1 gene, a UNG gene, an OGG1 gene, an MPG gene, an ERCC1 gene, an ERCC6 gene, a GPX1 gene, an MLH1 gene, an MSH6 gene, an SOD gene, an MKP-1 gene and a DDC gene, improving the anti-glycation activity, inhibiting cell fat accumulation, promoting lipolysis, improving the skeletal muscle basal metabolic rate, resisting depression, inhibiting melanogenesis, improving the anti-ultraviolet ability of skin, improving SOD activity, reducing ROS expression quantity, improving granulocyte activity and improving oxidation resistance. The flammulina velutipes extract is obtained by extracting flammulina velutipes with a solvent, and the solvent is water, alcohol or an alcohol-water mixture.

Description

technical field [0001] The invention relates to the field of plant extracts, in particular to the use of daylily flower extracts for regulating gene expression, skin care, anti-depression and cerebrovascular health care. Background technique [0002] Fat is an essential component in the human body, but excessive fat content will cause damage to the human body. In countries where the national economy is taking off more and more, the problem of obesity (also known as metabolic syndrome) will only increase. Therefore, from the perspective of the Asian region, China will be the country where the next wave of obesity problems will increase significantly. Therefore, obesity is an urgent problem to be solved. [0003] In addition, glycation is related to skin and internal aging, and it is also related to diabetes, which is very harmful to the health of Chinese people. The essence of the saccharification reaction is the Maillard reaction (Maillard reaction) or carbonyl-amine brown...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896A61K8/9794A23L33/105A61P17/18A61P25/24A61P39/06A61Q17/04A61Q19/00A61Q19/08
CPCA61K36/896A61K8/9794A61P25/24A61P39/06A61P3/04A61P17/18A61Q19/08A61Q17/04A61Q19/004A23L33/105A23V2002/00A23V2250/21A23V2200/30A23V2200/3324A23V2200/316A23V2200/318A23V2200/322A23V2200/332
Inventor 林咏翔
Owner TCI CO LTD
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