Polypeptide mixture with oxidation resistance and DPP-IV inhibitory activity and preparation method of polypeptide mixture

A DPP-IV, anti-oxidation technology, applied in the field of food processing, can solve the problems of complex denaturation mechanism and difficulty in accurately preparing bioactive peptides

Active Publication Date: 2021-04-20
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, since whey protein contains a wide variety of proteins, the existence of various proteins makes the denaturation mechanism induced by ultra-high pressure very complicated, which makes it difficult to accurately prepare bioactive peptides with specific functional activities

Method used

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  • Polypeptide mixture with oxidation resistance and DPP-IV inhibitory activity and preparation method of polypeptide mixture
  • Polypeptide mixture with oxidation resistance and DPP-IV inhibitory activity and preparation method of polypeptide mixture
  • Polypeptide mixture with oxidation resistance and DPP-IV inhibitory activity and preparation method of polypeptide mixture

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preparation example Construction

[0041] The present invention also provides a preparation method of a polypeptide mixture having antioxidant and DPP-IV inhibitory activity, the preparation method comprising the following steps:

[0042] Step 1: subject the whey protein isolate solution with a concentration of 1% to ultra-high pressure treatment to obtain an ultra-high pressure isolated whey protein solution;

[0043] Step 2: adjust the pH of the ultra-high pressure separated whey protein solution described in step 1 to 2.0-3.0, then add pepsin and acid protease, heat and hydrolyze, inactivate the enzyme, centrifuge to take the supernatant, and freeze-dry after rotary evaporation to obtain crude peptide mixture;

[0044] Step 3: adjust the concentration of the crude polypeptide mixture to 1 mg / mL, and separate and purify it by gel filtration chromatography and RP-HPLC.

[0045] Specifically, in step 1, the pressure of the ultra-high pressure treatment is 200-600 MPa, and the time of the ultra-high pressure tr...

Embodiment 1

[0067] The present invention provides a polypeptide mixture with antioxidant and DPP-IV inhibitory activity. The specific source, amino acid sequence, site, ABTS free radical scavenging rate and DPP-IV inhibitory rate of the polypeptide mixture are shown in Table 1.

[0068] The amino acid sequence of the polypeptide mixture includes: HLVDEPQNLIK, DDQNPHSSN, DDEVDVDGTVEEDLGK.

[0069] The peptide mixture is derived from whey protein and has 14 peptides in total.

Embodiment 2

[0071] The present invention also provides a preparation method of a polypeptide mixture having antioxidant and DPP-IV inhibitory activity, the preparation method comprising the following steps:

[0072] Step 1: prepare a whey protein isolate solution with a concentration of 1% (w / w) with deionized water, divide the whey protein isolate solution into polyethylene bags and vacuum pack them, and place them in an ultra-high pressure container. Control the circulating water system in the ultra-high pressure vessel, so that the ultra-high pressure treatment is carried out under the conditions of the temperature in the vessel being 31°C, the ultra-high pressure boosting rate of 8.3MPa / min, the treatment pressure of 200-600MPa, and the treatment time of 5-30min to obtain ultra-high pressure Processed whey protein solution;

[0073] Step 2: Use 1mol / L hydrochloric acid to adjust the pH value of the whey protein isolate solution after the ultra-high pressure treatment in step 1 to 2.0-...

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Abstract

The invention discloses a polypeptide mixture with oxidation resistance and DPP-IV (Dipeptidyl Peptidase IV) inhibitory activity. The amino acid sequences of the polypeptide mixture comprise HLVDEPQNLIK, DDQNPHSSN and DDEVDVDGTVEEDLGK. The polypeptide mixture is derived from whey protein. The preparation method of the polypeptide mixture comprises the following steps: step 1, carrying out ultrahigh-pressure treatment on a whey protein isolate solution with the concentration of 1% to obtain an ultrahigh-pressure whey protein isolate solution; step 2, adjusting the pH value of the ultrahigh-pressure whey protein isolate solution in the step 1 to 2.0-3.0, adding pepsase and acid protease, carrying out heating hydrolysis and enzyme deactivation, carrying out centrifugation to obtain a supernatant, and carrying out freeze drying after rotary evaporation to obtain a polypeptide mixture crude product; and step 3, adjusting the concentration of the polypeptide mixture crude product to 1mg / mL, and separating and purifying the polypeptide mixture crude product by adopting gel filtration chromatography and RP-HPLC. The polypeptide mixture disclosed by the invention is high in oxidation resistance and DPP-IV inhibitory activity, simple in preparation process, easy to operate, environment-friendly and pollution-free.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a polypeptide mixture with antioxidant and DPP-IV inhibitory activity and a preparation method thereof. Background technique [0002] Cells in the human body produce a large number of free radicals and reactive oxygen species during the metabolic process, and the antioxidant system can effectively remove free radicals. When free radicals are overproduced or antioxidant systems fail, this balance is disrupted and the body is left in a state of oxidative stress, a condition that can lead to cellular damage. Studies have shown that persistent oxidative stress is the cause of neurodegenerative diseases, cancer, liver injury, aging, cardiovascular disease, chronic pancreatitis and other diseases. Cells generally protect themselves from oxidative damage by preventing, repairing, producing antioxidants, or consuming dietary antioxidants. Known dietary antioxidants ...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K7/08C12P21/06C07K1/16C07K1/20A61P39/06A61P3/10
Inventor 郑远荣刘振民庞佳坤徐致远苏米亚刘景洪青焦晶凯
Owner BRIGHT DAIRY & FOOD
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