Construction and application of fimbriae-free escherichia coli capable of improving production efficiency

A technology of Escherichia coli and recombinant Escherichia coli, applied in the fields of genetic engineering and fermentation engineering, can solve the problems of slow growth of L-threonine fermentation, consumption of large carbon sources, and inability to meet the requirements
CN112680393AActive Publication Date: 2021-04-20JIANGNAN UNIV
2 Cites 7 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2021-04-20

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses construction and application of fimbriae-free escherichia coli capable of improving production efficiency, and belongs to the field of gene engineering and fermentation engineering. 64 genes of a fimbriae gene cluster on an escherichia coli genome are knocked out to obtain mutant bacteria WQM026, the strain grows faster in a nutrient-deficient culture medium, and the total amount of thalli is increased. The related genes synthesized by PHB and L-threonine are respectively transformed into the strain WQM026, and the obtained recombinant bacteria WQM026 / pBHR68 and WQM026 / pFW01-thrA*BC-rhtC can be used for respectively and efficiently synthesizing PHB and L-threonine. The synthesized PHB accounts for 87.87% of the dry weight of the cells and is 3.44 times that of wild type control bacteria. The yield of the L-threonine is 2.49 g / L and is 3.66 times that of wild type control bacteria.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the construction and application of a pili-free Escherichia coli capable of improving production efficiency, and belongs to the fields of genetic engineering and fermentation engineering. Background technique

[0002] Bacterial pili are large, long, and thin supramolecular protein appendages that arise on cells and are responsible for biofilm formation, chemotaxis, adhesion, and DNA transmembrane transfer. Pili are also an important component of bacterial biofilms and have multiple adverse effects on industrial activities such as increasing the risk of product spoilage or contamination, causing serious infections, forming dormant cells to increase antibiotic resistance, depleting energy and substrates of bacteria , Block the diffusion of nutrients, form biofouling, reduce heat transfer, increase corrosion, and shorten the service life of fermentation equipment.

[0003] Polyhydroxyalkanoates (PHAs) are synthesized from a varie...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More