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Streptomyces lavendulae 2-1-2F-1 and application of biomass of Streptomyces lavendulae 2-1-2F-1 in prevention and treatment of soil-borne diseases of vegetables

A technology for Streptomyces lilacinus, soil-borne diseases, applied in the fields of pest control, microorganism-based methods, chemicals for biological control, etc. Not ideal, etc., to achieve the effect of low cost, good effect, and simple screening method

Active Publication Date: 2021-04-30
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the different sensitivities of pathogenic bacteria in different crops to pesticides, the control effect on vegetable soil-borne diseases in production is not ideal.

Method used

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  • Streptomyces lavendulae 2-1-2F-1 and application of biomass of Streptomyces lavendulae 2-1-2F-1 in prevention and treatment of soil-borne diseases of vegetables
  • Streptomyces lavendulae 2-1-2F-1 and application of biomass of Streptomyces lavendulae 2-1-2F-1 in prevention and treatment of soil-borne diseases of vegetables
  • Streptomyces lavendulae 2-1-2F-1 and application of biomass of Streptomyces lavendulae 2-1-2F-1 in prevention and treatment of soil-borne diseases of vegetables

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Isolation of Actinomycetes with Strong Antagonistic Activity to Cucumber and Tomato Fusarium wilt.

[0040] Randomly collect soil samples from various places in Jiangsu. The sampling method is to shovel off the top 5cm soil layer, take 50g of fresh soil samples, store them in fresh-keeping bags, and record the sampling location and date;

[0041] Take 0.5g of soil sample and add 1mL of water, shake to make soil suspension, dilute 10 3 ~10 5 times, take 100 μl each of the dilutions to smear on the plate, and incubate in a 28°C incubator for 48 hours;

[0042] Pick a single colony, streak culture on Gao's No. 1 medium, purify and save it for later use;

[0043] Cucumber and tomato Fusarium wilt were used as target bacteria, and the antagonistic activity of the isolated strains was determined by confrontation culture method. After culturing at 24°C for 4-5 days, the inhibitory bandwidth was measured. The results showed that the strain 2-1-2F-1 had a strong inhi...

Embodiment 2

[0044] Example 2: Classification and Identification of Bacterial Strain 2-1-2F-1

[0045] (1) Morphological characteristics of strain 2-1-2F-1

[0046] Strain 2-1-2F-1 grew well on Gaoshi No. 1 solid medium, and the aerial hyphae and basal hyphae both grew abundantly ( figure 2 Middle A), the colony is round and concentric rings, the hyphae are powdery, the hyphae at the edge are radial, and multi-branched. With the differentiation and maturation of sporocydus and spores, the aerial hyphae gradually change from white to lavender grey( figure 2 Middle B), the hyphae in the base are white at the initial stage, and gradually turn into milky yellow in the later stage, and do not produce soluble pigments ( figure 2 Middle C). Strain 2-1-2F-1 was observed under an optical microscope and found that the spore filaments coexisted in straight and helical shapes, and there were many linear spore filaments. figure 2 Middle D-E); the surface of the spores is smooth, round to oval, ...

Embodiment 3

[0053] Example 3: Analysis of the properties and components of the antibacterial active substances produced by bacterial strain 2-1-2F-1

[0054] Strain 2-1-2F-1 was fermented in No. 4 fermentation broth at 30°C and 180r / min for 5 days, centrifuged at 5000r / min for 20min, and the supernatant was taken, and 4 times the volume of -20°C pre-cooled Acetone, mix by inversion, overnight. Centrifuge at 5000r / min for 30min at 4°C, remove the supernatant, and air-dry at 0°C. Redissolved with organic solvents of different polarities. It was found that the antibiotic was insoluble in organic solvents such as acetonitrile, methanol, n-butanol, ethyl acetate, chloroform, and petroleum ether, except that it was slightly soluble in dimethyl sulfoxide, but could be completely dissolved. Yu Shui ( Figure 4 ). Dissolve the antibiotic substance with PBS of 1 / 100 of the original volume, and measure the antibacterial ability of the antibacterial active substance by the confrontation culture me...

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Abstract

The invention provides Streptomyces lavendulae 2-1-2F-1 and application of the biomass of the Streptomyces lavendulae 2-1-2F-1 in prevention and treatment of soil-borne diseases of vegetables, wherein the preservation number of the Streptomyces lavendulae 2-1-2F-1 is CGMCC No: 19768. According to the invention, the Streptomyces lavendulae 2-1-2F-1 has stable natural raw material sources, the strain comes from soil, and the screening method is simple; culture medium raw materials used for fermentation, such as soybean cake powder and glucose, are low in cost; the antibacterial active substance is simple to prepare and convenient to operate; and antibiotic substances come from the nature, are green and environmentally friendly, and have a good effect of preventing and treating soil-borne diseases of vegetables.

Description

technical field [0001] The invention relates to the biological control of plant diseases, belongs to the field of biological control research and application in the plant protection discipline, and specifically relates to a new Streptomyces lavender gray and the application of its anti-living active substances. Background technique [0002] With the adjustment of industrial structure and the increase of people's demand for fresh vegetables, the planting area of ​​protected vegetables is increasing year by year. High fertilizer, continuous cropping, and multi-crop planting provide the possibility for the accumulation of plant pathogens in the soil and the outbreak of soil-borne diseases. , Destructive soil-borne diseases occur from time to time in the vegetable production areas of various facilities. At present, the prevention and control of soil-borne diseases of facility vegetables mainly relies on chemical agents. Over-frequency and excessive application of chemical agent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/28A01P3/00A01P1/00A01P21/00C12R1/465
CPCC12N1/20A01N63/28
Inventor 陈夕军吴冰越陈宸
Owner YANGZHOU UNIV
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