Method for preparing aged Dao dish by adopting direct vat set fungicide
A direct-injection, bacterial agent technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria used in food preparation, etc., can solve the problems of difficult product taste, strong wine taste, long fermentation period, etc. , to achieve the effect of blocking the formation of nitrite, strong tolerance and rapid acid production
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[0036] The preparation method of the direct injection bacterial agent: after activating the Lactobacillus plantarum, insert 3% of the total volume into the expansion culture medium to continue the cultivation. The components of the expansion culture medium are: 2.5-3.0% of glucose, 0.3-0.6% of yeast powder, and 0.5-1.0% of beef extract. The culture conditions are as follows: the initial pH of the culture medium is 6.0-6.5, the culture temperature is 30-35° C., the rotation speed of the shaker is 80-100 r / min, and the culture time is 12-16 hours. Centrifuge the culture solution after the above expansion at a temperature of 4°C and a speed of 4000r / min for 20 minutes to obtain the bacteria sludge, and add a protective agent. In terms of mass, the ratio of the protective agent to the bacteria sludge is 1: 2-3. Vacuum freeze-drying to obtain bacterial powder. Among them, the protective agent includes the following raw materials in parts by weight: 9 parts of skimmed milk powder, ...
Embodiment 1
[0042] The preparation method of the direct injection bacterial agent: after activating the Lactobacillus plantarum, insert 3% of the total volume into the expansion culture medium to continue the cultivation. The components of the expansion medium are: 2.8% glucose, 0.5% yeast powder, and 0.8% beef extract. The culture conditions are as follows: the initial pH of the medium is 6.2, the culture temperature is 32° C., the rotation speed of the shaker is 100 r / min, and the culture time is 14 hours. Centrifuge the culture solution after the above expansion at a temperature of 4°C and a speed of 4000r / min for 20 minutes to obtain the bacteria sludge, and add a protective agent. In terms of mass, the ratio of the protective agent to the bacteria sludge is 1: 2.5, vacuum freeze-drying to obtain bacterial powder. Among them, the protective agent includes the following raw materials in parts by weight: 9 parts of skim milk powder, 2 parts of trehalose, 0.6 parts of glycerin, 2.5 part...
Embodiment 2
[0050] A kind of method that adopts direct-throwing bacterial agent to make aged dish, concrete steps are as follows:
[0051] (1) Select 3000g of fresh and non-corruption mature stick vegetables, cut off the stem bark of the rod-shaped part with a knife, wash it with clean water, and drain it.
[0052] (2) cut the stick vegetables after step (1) into small pieces with a knife, place them in a large basin, add 240g of edible salt and 0.6g direct-throwing type bacterial agent (the preparation method of the bacterial agent is the same as in Example 1) , mix well and put it in a container such as a jar or a bag, and ferment for 24 hours in an airtight manner.
[0053] (3) Remove the fermented spinach from the container in step (2), discard the liquid in the container, knead it for 1 hour to make the texture of the spinach soft, put it in a large basin, add 1.5g direct-injection bacterial agent, Put it in the container of step (2) after fully mixing, and ferment for 24 hours in a...
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