Preparation method of microbial environment-friendly deodorant
An environmentally friendly deodorant and microorganism technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve good deodorization effect, wide application range and excellent effect
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Embodiment 1
[0040] A preparation method of microbial environment-friendly deodorant, comprising the steps of:
[0041] (1) Prepare medium:
[0042] Prepare MRS liquid medium, MRS medium LB medium, Erlenmeyer flask medium, seed tank medium, fermenter medium;
[0043] The components and mass percentages of the Erlenmeyer flask medium are: 3% glucose, 1.5% peptone, 1% yeast extract, 1% beef extract, and the rest is sterile ultrapure water; adjust the pH of the medium with sodium hydroxide is 6.6;
[0044] The components and mass percentages of the seed tank medium are: 10% molasses, 0.5% peptone, 0.2% yeast extract, 0.5% potassium dihydrogen phosphate, 0.2% manganese sulfate pentahydrate, and the rest is sterile ultrapure water. Sodium hydroxide adjusted the pH of the medium to 6.6, and the components of the fermentor medium were consistent with those of the seed tank medium.
[0045] (2) Rejuvenation and activation of lactic acid bacteria:
[0046] (2-1) Inoculate the preserved lactic a...
Embodiment 2
[0059] The present embodiment is roughly the same as Example 1, and the difference is that in the microbial environment-friendly deodorant mother liquor obtained in step (6), the viable count of Bacillus subtilis is 0.8×10 9 cfu / mL, the number of live lactic acid bacteria is 1.2×10 9 cfu / mL.
Embodiment 3
[0061] The present embodiment is roughly the same as Example 1, and the difference is that in the microbial environment-friendly deodorant mother liquor obtained in step (6), the viable count of Bacillus subtilis is 3.0×10 9 cfu / mL, the number of live lactic acid bacteria is 2.0×10 9 cfu / mL.
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Abstract
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