High-density fermentation culture medium for lycopene-producing saccharomyces cerevisiae

A technology of high-density fermentation and Saccharomyces cerevisiae, which is applied in the field of high-density fermentation medium and can solve problems such as optimization

Active Publication Date: 2021-05-11
GUANGDONG BOWOTE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In basic research and some industrial production, Saccharomyces cerevisiae mostly uses Yeast Extract PeptoneDextrose (YEPD) medium, and its medium components have not been optimized for specific Saccharomyces cerevisiae strains

Method used

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  • High-density fermentation culture medium for lycopene-producing saccharomyces cerevisiae
  • High-density fermentation culture medium for lycopene-producing saccharomyces cerevisiae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Comparison of single factor test results of optimum carbon source and optimum nitrogen source shake flask fermentation in the culture medium

[0032] Determination of the most suitable carbon source

[0033] Basic fermentation medium 1 composition: carbon source 2%, peptone 2%, KH 2 PO 4 0.1%, pH7.0. Cane molasses liquid, glucose, sucrose, soluble starch or maltose were added as carbon sources (2%) to the basal fermentation medium 1 respectively.

[0034] Determination of optimal nitrogen source

[0035] Composition of fermentation medium 2: sugarcane molasses liquid 2%, nitrogen source 2%, KH 2 PO 4 0.1%, pH7.0. An appropriate amount of nitrogen source was added on the basis of the selected optimum carbon source (sugarcane molasses liquid). Peptone, corn steep liquor dry powder, soybean meal powder, bran powder, ammonium sulfate or urea were added to fermentation medium 2 as nitrogen source (2%) instead.

[0036] The preparation method of the cu...

Embodiment 2

[0044] Embodiment 2: Configuration of fermentation medium 1 and comparison of fermentation results

[0045] Saccharomyces cerevisiae high-density fermentation medium composition: according to the ratio per liter, sugarcane molasses liquid 35g / L, corn steep liquor dry powder 20g / L, potassium dihydrogen phosphate 1.5g / L, MgSO 4 . 7H 2 O 3g / L, potassium sulfate 5g / L.

[0046] The preparation method of the culture medium is as follows: mix the above ingredients evenly, dissolve them with distilled water, put the prepared culture medium into a fermenter, stir evenly, and sterilize at 115° C. for 20 minutes under high temperature and high pressure.

[0047] In order to achieve the best effect of the medium, when using the above-mentioned Saccharomyces cerevisiae high-density fermentation medium to ferment Saccharomyces cerevisiae, the fermentation process is as follows:

[0048] The main operating steps of the high-density fermentation of the present embodiment are as follows:

...

Embodiment 3

[0063] Embodiment 3: the preparation of fermentation medium 2 and the comparison of fermentation result

[0064] Saccharomyces cerevisiae high-density fermentation medium composition: according to the ratio per liter, sugarcane molasses liquid 30g / L, corn steep liquor powder 20g / L, potassium dihydrogen phosphate 1.5g / L, MgSO 4 . 7H 2 O 3g / L, potassium sulfate 5g / L.

[0065] The preparation method of the culture medium is as follows: mix the above ingredients evenly, dissolve them with distilled water, put the prepared culture medium into a fermenter, stir evenly, and sterilize at 115° C. for 20 minutes under high temperature and high pressure.

[0066] In order to achieve the best effect of the medium, when using the above-mentioned Saccharomyces cerevisiae high-density fermentation medium to ferment Saccharomyces cerevisiae, the fermentation process is as follows:

[0067] The main operating steps of the high-density fermentation of the present embodiment are as follows: ...

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Abstract

The invention discloses a high-density fermentation culture medium of lycopene-producing saccharomyces cerevisiae. The preservation number of the saccharomyces cerevisiae PDC-erg9 disclosed by the invention is GDMCC No: 61352. The high-density fermentation culture medium for the lycopene-producing saccharomyces cerevisiae is prepared from 30 to 40g/L of cane molasses liquid, 20 to 25g/L of corn steep liquor dry powder, 1.5 to 2g/L of monopotassium phosphate, 3 to 3.5 g/L of MgSO4.7H2O, 5 to 6g/L of potassium sulfate and the balance of water. When the culture medium is used for culturing the saccharomyces cerevisiae, the bacterium concentration can reach 1.51*10<9>cfu/ml, and the lycopene concentration can reach 280mg/L; and higher-density thalli are effectively obtained, the expression quantity of target secondary metabolites is improved, the production cost is greatly reduced, and the production efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a high-density fermentation medium of Saccharomyces cerevisiae producing lycopene and its application. Background technique [0002] The animal husbandry industry in my country is developing rapidly. Antibiotics have strong advantages in treating animal diseases and promoting animal growth. However, due to unfavorable factors such as drug residues and drug resistance, their use in the world is gradually restricted. Therefore, the research and development of safe and effective alternatives to antibiotics has become a hot and difficult issue that enterprises related to livestock and poultry farming urgently need to solve. [0003] With the enhancement of people's awareness of environmental protection and animal product safety, antibiotic substitutes have become a hot spot. Lycopene can effectively inhibit the occurrence of harmful oxidation in animals, p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C12N1/19C12R1/865
CPCC12P5/007C12N1/18
Inventor 朱红惠胡艳娜苏卜利周莲谢小林陈猛
Owner GUANGDONG BOWOTE BIOTECH CO LTD
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