Preparation method of multi-stage bionic nano-drug carrier with targeted long circulation

A biomimetic nanotechnology, long-circulation technology, applied in nano-drugs, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc., can solve the problems of short cycle time and insufficient function

Active Publication Date: 2021-05-14
SHIHEZI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Mesoporous silica has the characteristics of high porosity, high specific surface area, thermal stability, large drug loading capacity, stable structure, good biocompatibility, and no toxic and side effects, but its circu

Method used

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  • Preparation method of multi-stage bionic nano-drug carrier with targeted long circulation
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  • Preparation method of multi-stage bionic nano-drug carrier with targeted long circulation

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preparation example Construction

[0036] A method for preparing a multi-level biomimetic nano drug carrier with targeted long circulation, comprising the following steps:

[0037] (1) Preparation of mesoporous silica.

[0038] (2) Loading anticancer drugs: adding the mesoporous silica and anticancer drugs into water, and stirring for 12 hours, to obtain mesoporous silica loaded with anticancer drugs.

[0039] (3) Modification of erythrocyte membrane: distearoylphosphatidylethanolamine-polyethylene glycol-biotin and broken erythrocyte membrane were stirred at 4°C for 12 hours, and then extruded continuously through a 200nm filter membrane with a micro extruder, Biotin-modified erythrocyte membranes were obtained.

[0040] Erythrocyte membranes have the advantage of high biocompatibility.

[0041] The biotin was stirred at 5° C. for 12 hours to modify the biotin on the red blood cell membrane, so that the nanoparticles were targeted, thereby improving the utilization efficiency of the nanoparticles loaded with...

Embodiment 1

[0057] The specific operation steps are as follows:

[0058] (1) Preparation of mesoporous silica:

[0059] Add 2 parts of n-amyl alcohol, 4 parts of TEOS (tetraethyl orthosilicate) into 30 parts of cyclohexane and stir evenly, add 2.44 parts of CTAB (cetyltrimethylammonium bromide) and 30 parts of deionized water, After stirring, it was transferred to a polytetrafluoroethylene reactor, placed in an oven at 120°C for 2 hours, washed three times with deionized water, dried in air at 70°C for one day to obtain a white powder, and then sintered in a muffle furnace at 550°C for 6 hours, namely Mesoporous silica (MSN) is available as a white powder.

[0060] (2) Preparation of mesoporous silica loaded with anticancer drug DOX:

[0061] Add mesoporous silica and anticancer drug DOX into water (the mass ratio of mesoporous silica, anticancer drug DOX and water is 5:1:20), stir for 12 hours, and load anticancer drugs by electrostatic interaction. Mesoporous silica loaded with antic...

Embodiment 2

[0070] The method of step (1) in Example 1 was used to prepare mesoporous silica. As a drug carrier, the size of mesoporous silica should not be too large or too small, preferably between 100-200nm. Therefore, the mesoporous silica prepared in Example 1 is the best drug carrier.

[0071] Mesoporous silicon dioxide prepared in Example 1 is tested for drug loading performance, and the drug loading can reach about 30%, which realizes the high drug loading of mesoporous silicon and improves the loading efficiency (in the prior art, conventional The drug loading of the drug carrier is 5-8%). Using the method of Example 1, the anticancer drug DOX was loaded on the mesoporous silica, and the different concentrations of the anticancer drug DOX were regulated, and finally the concentration of DOX was 450 μg / mL when the drug loading was maximum (such as image 3 As shown), the drug loading effect is good, which provides the possibility for future loading of drugs or other macromolecul...

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Abstract

The invention relates to a preparation method of a multi-stage bionic nano-drug carrier with targeted long circulation. The invention relates to a preparation method of a multi-stage bionic nano-drug carrier with targeted long circulation. The preparation method comprises the following steps: (1) preparing mesoporous silica; (2) loading an anti-cancer drug to the mesoporous silica; (3) modifying an erythrocyte membrane by adopting biotin; and (4) wrapping the mesoporous silica loaded with the anti-cancer drug by using the biotin-modified erythrocyte membrane. According to a technical scheme in the invention, synthesized nanoparticles can effectively avoid a biological barrier through wrapping of the erythrocyte membrane and enter blood circulation of the whole body, and efficient accumulation of the nanoparticles in tumors is achieved; and active targeting is carried out by modifying biotin, so the aggregation of a drug carrier in a tumor part is further improved, the biocompatibility of the drug carrier is greatly improved, the circulation time of the drug carrier in a human body is greatly prolonged, the aggregation effect of the drug carrier in a tumor area is improved through the targeting effect, and the drug carrier can be used for delivering drugs in cancer cells.

Description

technical field [0001] The invention specifically relates to a preparation method of a multi-level biomimetic nano-medicine carrier with targeted long circulation. Background technique [0002] Cancer is one of the main causes of human death. About 8.8 million people die of cancer every year in the world, and one person is diagnosed with cancer every 7 minutes. Therefore, finding more effective cancer treatment methods has become a major issue that people need to tackle. At present, chemotherapy and radiotherapy are the two mainstream treatments for cancer at this stage, but their further development is limited due to low efficiency and severe side effects. [0003] In the treatment of nano-tumors, nano-materials are synthesized by chemical methods as drug carriers, which reduces the side effects of drugs on the human body and can achieve the purpose of efficiently killing tumor cells. However, when nanoparticles enter the body, the body's non-specific immune system will r...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K47/46A61K47/69A61K45/00A61K47/02A61K47/54A61K47/60A61P35/00B82Y5/00B82Y30/00B82Y40/00
CPCA61K9/5068A61K47/6901A61K47/60A61K47/545A61K47/02A61K45/00A61P35/00B82Y5/00B82Y30/00B82Y40/00
Inventor 杨盛超崔林张一凡刘志勇吴建宁孟桂花李文娟杨怡平林富丽乔智强
Owner SHIHEZI UNIVERSITY
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