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Banana growth-promoting probiotic agent produced by fermenting passion fruit peel and preparation method

The technology of passion fruit peel and probiotics is applied in the field of banana growth-promoting probiotics and preparation, and can solve the problem of no passion fruit peel fermentation and the like, and achieve the effects of preventing banana fusarium wilt, having broad market prospects and high application value.

Pending Publication Date: 2021-05-14
南宁市拜欧生物工程有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After reviewing the relevant literature, there is no use of passion fruit peel fermentation to produce banana growth-promoting probiotics

Method used

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  • Banana growth-promoting probiotic agent produced by fermenting passion fruit peel and preparation method

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Experimental program
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Effect test

Embodiment 1

[0038] In this embodiment, the mixing ratio of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma, and Enterococcus faecalis was investigated, and different proportions of Bacillus subtilis and Paecilomyces lilacinus were mixed according to the inoculation amount of 5% (V / W). Add 3% glucose, 34% water, 10% bean dregs, 0.6% urea, 0.03% dipotassium hydrogen phosphate, and 0.03% magnesium ion to the dried passion fruit peel residue for mixing. In a solid fermentation medium, put in a fermenter, the fermentation temperature is 37 ° C, 28 ° C, 28 ° C, 35 ° C, the fermentation time is 24 hours, the mixture of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma, and Enterococcus faecalis The ratio is 0.4:0.3:2:0.02, 0.2:0.5:2:0.02, 0.2:0.3:4:0.02, 0.2:0.3:2:0.04 to measure the plant height, pseudostem circumference and average length of the longest root , the number of new tap roots, and the number of new leaves. Compared with the results without adding the probiotic, it can b...

Embodiment 2

[0040]In this embodiment, the mixing ratio of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma, and Enterococcus faecalis was investigated, and different proportions of Bacillus subtilis and Paecilomyces lilacinus were mixed according to the inoculation amount of 5% (V / W). Add 3% glucose, 34% water, 10% bean dregs, 0.6% urea, 0.03% dipotassium hydrogen phosphate, and 0.03% magnesium ion to the dried passion fruit peel residue for mixing. In a solid fermentation medium, placed in a fermenter, the fermentation temperature is 37°C, 28°C, 28°C, 35°C, and the fermentation time is 24h. The mixing ratios of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma, and Enterococcus faecalis are 0.4:0.4:3:0.02, 0.3:0.5:2:0.02, 0.2:0.3:4:0.02, 0.2:0.3:2 :0.04 Observing the situation of Fusarium wilt in bananas, i.e. inoculation of Fusarium oxysporum f.sp.cubense (FOC), the pathogenic bacterium of Fusarium wilt of bananas, from the appearance of the first strain of diseased plants on...

Embodiment 3

[0042] In this embodiment, the mixing ratio of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma, and Enterococcus faecalis was investigated, and different proportions of Bacillus subtilis and Paecilomyces lilacinus were mixed according to the inoculation amount of 5% (V / W). Add 3% glucose, 34% water, 10% bean dregs, 0.6% urea, 0.03% dipotassium hydrogen phosphate, and 0.03% magnesium ion to the dried passion fruit peel residue for mixing. In a solid fermentation medium, placed in a fermenter, the fermentation temperature is 37°C, 28°C, 28°C, 35°C, and the fermentation time is 24h. The mixing ratios of Bacillus subtilis, Paecilomyces lilacinus, Trichoderma, and Enterococcus faecalis are 0.4:0.2:3:0.02, 0.2:0.5:2:0.02, 0.2:0.3:4:0.02, 0.3:0.3:3 : 0.04 Determination of aerial part biomass (in terms of fresh weight and dry weight), wherein the dry weight measurement method is to pull out the banana plant from the nutrient bowl, wash off the root matrix under tap water, dry i...

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Abstract

The invention discloses a banana growth-promoting probiotic agent produced by fermenting passion fruit peels and a preparation method, the banana growth-promoting probiotic agent comprises the following components: bacillus subtilis, paecilomyces lilacinus, trichoderma and enterococcus faecalis; the bacillus subtilis, the paecilomyces lilacinus, the trichoderma and the enterococcus faecalis are mixed according to the mass ratio of (0.1-0.4): (0.1-0.5): (1-4): (0.01-0.04). The invention relates to the technical field of value-added utilization of agricultural and sideline products and microbial agents. According to the banana growth-promoting probiotic agent produced by fermenting the passion fruit peel and the preparation method, the prepared microbial agent has an obvious growth-promoting effect on bananas, has an obvious promoting effect on growth of banana rhizomes and increase of the total weight of plants on the ground, can prevent banana wilt to a great extent and promote healthy growth of the bananas, and is high in fermentation speed, low in cost, simple in preparation process, economical, environmentally friendly, high in application value and wide in market prospect, and the current situation that passion fruit peel resources are wasted and the environment is polluted is improved to a great extent.

Description

technical field [0001] The invention relates to the technical field of value-added utilization of agricultural and sideline products and microbial agents, in particular to a banana growth-promoting probiotic agent produced by fermenting passion fruit peel and a preparation method. Background technique [0002] Passion fruit, scientific name Passiflora edulis Sims, also known as egg fruit, belongs to Passiflora, is widely planted in tropical and subtropical regions, mainly yellow and purple fruits. Passion fruit fruit contains 17 kinds of amino acids, multivitamins and carotenoids needed by the human body, including very rich natural vitamin C and vitamins A, B1, B2, etc., and also rich in calcium, phosphorus, iron and various Amino acids and other substances and trace elements, the roots, stems and leaves of passion fruit can be used as medicines, which can reduce inflammation and pain, promote blood circulation and strengthen the body, nourish yin and kidney, lower fat and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/22A01N63/20A01N63/38A01N63/30A01P21/00A01P3/00C12R1/125C12R1/79C12R1/885C12R1/01
CPCC12N1/20C12N1/14A01N63/22A01N63/20A01N63/38A01N63/30
Inventor 梁树华罗石林李晓明韦朝英候守海苏智兴陆迪韦函忠
Owner 南宁市拜欧生物工程有限责任公司
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