Application of oligomeric mannuronic acid salts in preparation of drugs and functional foods for delaying skin aging and regulating immunity/resisting inflammation

A technology of polymannuronic acid and functional food, applied in the field of medicine

Pending Publication Date: 2021-05-18
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Oligomannuronate is a kind of alginate polysaccharide isolated from brown algae. At present, there are no reports on its application in skin anti-aging and anti-inflammatory allergies at home and abroad.

Method used

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  • Application of oligomeric mannuronic acid salts in preparation of drugs and functional foods for delaying skin aging and regulating immunity/resisting inflammation
  • Application of oligomeric mannuronic acid salts in preparation of drugs and functional foods for delaying skin aging and regulating immunity/resisting inflammation
  • Application of oligomeric mannuronic acid salts in preparation of drugs and functional foods for delaying skin aging and regulating immunity/resisting inflammation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Extraction, preparation and analysis of oligomannuronate

[0023] Oligomannuronic acid salt among the present invention can adopt conventional technical means in this area to make, and the specific steps in the present embodiment are:

[0024] Prepare alginate (sodium alginate in this example) into a 10wt% aqueous solution, heat it to 80-90°C with 1wt% dilute hydrochloric acid, stir and degrade it for 4-5 hours, neutralize it with 10wt% sodium carbonate aqueous solution after cooling, and then Use 5wt% dilute hydrochloric acid to adjust the pH to about 3.65, centrifuge to collect the precipitate, dissolve it with 2mol / L NaOH, add 3 times the volume of 95wt% ethanol, collect the precipitate, dehydrate with absolute ethanol and dry to obtain polymannuronic acid sodium salt. The sodium salt of polymannuronic acid is prepared into aqueous solutions of different concentrations with pure water, and degraded by the Fenton method to obtain oligomeric mannuronic acids...

Embodiment 2

[0029] Example 2: Effect of oligomannuronate on the survival rate of HaCaT cells induced by UVA

[0030] In this example, the sodium oligomannuronic acid prepared in Example 1 was used. The specific steps are:

[0031] (1) Cell culture: HaCaT cells were inoculated in MEM complete culture medium (containing 100 U / mL penicillin, 100 U / mL streptomycin and 10% FBS), placed at 37°C, 5% CO 2 cultured in an incubator.

[0032] (2) 8,000 cells per well were planted in a 96-well plate, placed in a constant temperature cell incubator and incubated for 12 hours, then added different concentrations of sodium oligomannuronate and placed in a constant temperature cell incubator to continue incubation for 48 hours. After the incubation, replace the medium and give 30J / cm 2 UVA exposure. After placing in a constant temperature cell culture incubator for 12 hours, add 20 μL (5 mg / ml) MTT solution to each well, and incubate for 4 hours in the incubator. After the end, remove the liquid, a...

Embodiment 3

[0038] Example 3: Effect of oligomannuronate on UVA-induced mitochondrial membrane potential of HaCaT cells

[0039]In this example, the sodium oligomannuronic acid prepared in Example 1 was used. The specific experimental steps are:

[0040] (1) Cell culture: HaCaT cells were inoculated in MEM complete culture medium (containing 100 U / mL penicillin, 100 U / mL streptomycin and 10% FBS), placed at 37°C, 5% CO 2 cultured in an incubator.

[0041] (2) 8,000 cells per well were planted in a 96-well plate, placed in a constant temperature cell incubator and incubated for 12 hours, then added different concentrations of sodium oligomannuronate and placed in a constant temperature cell incubator to continue incubation for 48 hours. After the incubation, replace the medium and give 30J / cm 2 UVA exposure. Place in a constant temperature cell incubator and continue to incubate for 12 hours, remove the medium, wash once with PBS solution, add 150 μL of staining working solution, mix ...

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Abstract

The invention provides application of oligomeric mannuronic acid salts in preparation of drugs and functional foods for delaying skin aging and regulating immunity / resisting inflammation. According to the invention, the oligomeric mannuronic acid salts with different molecular weights are prepared by using algin as a raw material and obtained by using a chemical degradation method or a physical degradation method or a combination of the two degradation methods; and oligomeric mannuronic acid is neutralized to prepare the oligomeric mannuronic acid salts with different molecular weights, and molecular skeletons of the oligomeric mannuronic acid salts are linear oligosaccharide compounds formed by D-mannuronic acid (M) through linking of beta-1, 4 glucosidic bonds. The product disclosed by the invention is derived from algal polysaccharides, has the advantages of being abundant in resources, prone to industrialization, safe, effective, etc., and has a wide development and application prospect in the aspect of preparing drugs and functional foods for delaying skin aging and regulating immunity.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of oligomannuronic acid salt in the preparation of medicines for delaying skin aging and immunity and anti-inflammation and functional food. Background technique [0002] The number of patients suffering from skin photodamage has increased dramatically, and skin photodamage has become an important public health problem. The skin acts as a physiological barrier with a complex network of enzymatic and non-enzymatic antioxidant defenses that scavenge volatile reactive oxygen species (ROS) / free radicals into less toxic species, thereby maintaining cellular redox homeostasis. Long-wave ultraviolet (UVA) is stronger than medium-wave ultraviolet (UVB) and can penetrate deep into the dermis. UVA is also the main cause of premature skin aging. Excessive exposure of skin to UVA can induce the generation of intracellular ROS, directly or indirectly damage cell ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61K31/702A23L33/125A61K8/60A61K8/73A61P17/00A61P17/16A61P29/00A61P37/08A61P39/00A61P39/06A61Q19/00A61Q19/08
CPCA61K31/715A61K31/702A61P39/06A61P29/00A61P17/00A61P37/08A61P39/00A61P17/16A61K8/73A61K8/60A61Q19/08A61Q19/004A23L33/125A23V2002/00A23V2200/304A23V2200/318A23V2200/324
Inventor 郝杰杰李琼于广利白董慧张帅
Owner OCEAN UNIV OF CHINA
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