Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus plantarum SLB01 with inhibiting effect on enterobacter cloacae as well as derivative product and application of lactobacillus plantarum SLB01

A technology of Lactobacillus plantarum and Enterobacter cloacae, applied in the field of microbiology, can solve problems such as hidden health problems

Active Publication Date: 2021-05-18
广西爱生生命科技有限公司
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, streptococcus itself is a pathogenic bacteria, which may bring new health risks to the human body

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus plantarum SLB01 with inhibiting effect on enterobacter cloacae as well as derivative product and application of lactobacillus plantarum SLB01
  • Lactobacillus plantarum SLB01 with inhibiting effect on enterobacter cloacae as well as derivative product and application of lactobacillus plantarum SLB01
  • Lactobacillus plantarum SLB01 with inhibiting effect on enterobacter cloacae as well as derivative product and application of lactobacillus plantarum SLB01

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Lactobacillus plantarum SLB01 was screened from feces samples of healthy and long-lived elderly in Guangxi. Specifically, the screening and identification methods of the Lactobacillus plantarum SLB01 are as follows:

[0034] 1) Weigh 1.0 g of fresh feces sample and add it to 9.0 mL of sterile saline, shake and mix evenly to make a bacterial suspension. Take 5mL of the above bacterial suspension and add it to 45mL enrichment medium, and incubate anaerobically at 37°C for 7 days. The enrichment medium formula is: tryptone 10.0g, beef heart extract powder 9g, beef powder 4.0g, yeast powder 2.0g, glucose 11.0g, sodium chloride 2.5g, disodium hydrogen phosphate 1.5g, dipotassium hydrogen phosphate 1.0 g. Diammonium hydrogen citrate 1.0g, sodium acetate 2.5g, magnesium sulfate 0.1g, manganese sulfate 0.02g, Tween 801.0g, distilled water 1000mL, pH value 6.5, sterilize at 115°C for 20min.

[0035] 2) Gradually dilute the cultured enriched culture solution with sterile physio...

Embodiment 2

[0040] In vitro inhibition experiment of Enterobacter cloacae

[0041] Method 1: Plate antibacterial test

[0042] Enter Enterobacter cloacae into MRS liquid medium, activate anaerobic culture at 37°C to a concentration of 1×10 8 CFU / ml. Take 0.1ml of Enterobacter cloacae bacteria liquid in a sterile plate, quantitatively pour 14ml of MRS solid medium at a temperature of 40°C, mix the medium and the bacteria liquid evenly, let the plate solidify, and prepare the Enterobacter cloacae plate .

[0043] The strain to be tested was inserted into the MRS liquid medium and cultured anaerobically at 37°C for 48h. Centrifuge to remove the supernatant. Use sterile tweezers to soak the sterile circular filter paper in the supernatant for a while, take it out, stick the filter paper to the center of the Enterobacter cloacae plate, and cover it. Incubate the plate anaerobically at 37°C for 48 hours, observe whether there is a bacteriostatic zone around the filter paper sheet, and meas...

Embodiment 3

[0052] acid and bile salt test

[0053] Bacterial suspension preparation: transfer and activate the bacterial strain from the preserved slant twice, wash the bacterial body from the slanted surface with sterile saline to prepare a bacterial suspension, and adjust the concentration of the bacterial suspension to 1×10 9 CFU / ml.

[0054] Acid resistance test: Experimental group: Take 0.1ml of the bacterial suspension and add it to 2mL of phosphate buffer with a pH value of 3.0, and incubate anaerobically at 37°C for 3 hours; after the culture is completed, the bacterial solution is serially diluted and spread on the MRS plate for plate counting. Control group: Phosphate buffer with a pH value of 3.0 was replaced with sterile physiological saline, and the rest of the operations were the same. Survival was calculated according to Formula I.

[0055] Survival rate=(experimental group plate colony number / control group plate colony number)×100% formula I

[0056] Acid-resistant and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides lactobacillus plantarum SLB01 with an inhibiting effect on enterobacter cloacae as well as a derivative product and application of the lactobacillus plantarum SLB01, and belongs to the technical field of microorganisms. The invention provides Lactobacillus plantarum SLB01 with an inhibition effect on enterobacter cloacae, and the preservation number of the Lactobacillus plantarum SLB01 is GDMCCNo: 61178. In-vitro bacteriostasis experiments and in-vivo bacteriostasis experiments show that compared with conventional lactobacillus, the Lactobacillus plantarum SLB01 has a relatively strong inhibiting effect on enterobacter cloacae and has the characteristics of acid resistance and cholate resistance. According to the present invention, by continuously eating the food or the bacterial agent containing the Lactobacillus plantarum SLB01, the results show that the abundance of the enterobacter cloacae in the intestinal tract can be significantly reduced so as to achieve the purposes of regulating the intestinal flora balance and assisting the prevention and treatment of obesity and other diseases caused by the enterobacter cloacae.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of Lactobacillus plantarum SLB01 having an inhibitory effect on Enterobacter cloacae, derivative products and applications thereof. Background technique [0002] Enterobacter cloacae exists widely in nature and can be detected in human and animal feces, sewage, soil, and plants. In the human gut, Enterobacter cloacae, as one of the common gut microbes, resides in the gut together with other gut microbes. Enterobacter cloacae is an opportunistic pathogen. In a healthy intestinal tract, Enterobacter cloacae is restricted by healthy flora and will not cause disease; but when the host's immunity is low and the intestinal flora is imbalanced, it can cause respiratory tract, wound , urinary tract infection and bacteremia. The latest research shows that Enterobacter cloacae is also related to diseases such as obesity and diabetes, and is one of the boosting facto...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A23L33/135A23L2/38A61K35/747A61P3/04A61P31/04A61P3/00C12R1/25C12R1/225
CPCC12N1/20A23L33/135A23L2/382A61K35/747A61P3/04A61P31/04A61P3/00A23V2002/00A23V2400/145A23V2400/143A23V2400/181A23V2400/169A23V2200/3204A23V2200/332A61K2300/00
Inventor 肖桂龙林艳金冯恩辉
Owner 广西爱生生命科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com