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Ultraviolet-resistant antioxidant acinetobacter and application thereof
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A UV-resistant and anti-oxidation technology, applied in the field of microorganisms, can solve the problems that biofilms are easily affected by ultraviolet and visible light, death, poisoning, etc.
Active Publication Date: 2021-05-18
HEILONGJIANG UNIV
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[0004] The present invention aims to solve the problem that the biofilm in the existing ICPB process is easily affected by ultraviolet light and visible light, resulting in death or poisoning, and provides a strain of iminetobacter resistant to ultraviolet light and anti-oxidation and its application
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specific Embodiment approach 1
[0019] Embodiment 1: The Acinetobacter that is resistant to ultraviolet light and anti-oxidation in this embodiment is Acinetobacter enhanphotocatalysis C3, which is preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, and the preservation address is Chaoyang District, Beijing No. 3, No. 1 Yard, Beichen West Road, the preservation date is November 20, 2019, and the preservation number is CGMCC No.18984.
specific Embodiment approach 2
[0020] Specific embodiment 2: In this embodiment, the screening method of Acinetobacter C3 resistant to ultraviolet light and anti-oxidation: Take 300 mg of surface soil from the flower bed in the campus of Heilongjiang University, add distilled water to 1 L, stir evenly and then let stand. The supernatant after standing still is the soil leachate. Take 3 parts of 300mL soil leaching liquid and add 500mL screening liquid respectively, at 35℃, UV 254 Under the irradiation of ultraviolet light and magnetic stirrer, cultured for 3 days. After serial dilution, at 35°C, UV 254 Separation was carried out under the condition of ultraviolet light irradiation, and a typical single colony was selected for purification and culture, which was the strain C3 of this embodiment.
[0021] The screening liquid composition is: 1g / L carbon source, NH 4 Cl 0.1~0.5g / L, MgSO 4 ·7H 2 O 0.01~0.05g / L, K 2 HPO 4 0.05~0.2g / L, NaCl 0.05~0.12g / L, MnSO 4 4H 2 O 0.01g / L, FeSO 4 0.01g / L, 0.5~1g / L...
specific Embodiment approach 3
[0027] Specific embodiment three: In this embodiment, strong photocatalytic Acinetobacter (Acinetobacterenhanphotocatalysis) C3 is activated under ultraviolet light UV 254 Growth test under irradiation conditions:
[0028]The ultraviolet light is 254nm, and the intensity of the ultraviolet light is 27.8μW / cm 2 , 35°C, using standard LB medium (tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L) to cultivate the strain Acinetobacter enhanphotocatalysisC3, by measuring the absorbance value (OD 600 ) to draw the growth curve of the strain, and compare it with the growth curve of the strain C3 cultivated under the conditions of no light and 35°C, the results are as follows image 3 As shown, ● in the figure indicates the ultraviolet light condition, and ■ indicates the dark culture condition. UV light 254 Under irradiation conditions, the adaptation period of strain C3 was shortened, which was related to the decomposition of organic matter in water by ultraviolet light, which made ...
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technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Acinetobacter resistant to ultraviolet light and anti-oxidation and its application. Background technique [0002] In the early 21st century, Bruce E. Rittmann first proposed the direct coupling technology of photocatalysis and biodegradation (Intimated Coupling of Photocatalysis and Biodegradation, ICPB). The technique is to grow biofilms on TiO-loaded 2 In the porous carrier framework, prevent microorganisms from being affected by TiO 2 The UV rays required for photocatalysis and the attack of hydroxyl radicals produced by photocatalysis, and TiO 2 The biodegradable part of the photocatalytic degradation product is immediately degraded by the biofilm, while the non-biodegradable part is further oxidized by photocatalysis, thereby achieving rapid and thorough mineralization of organic pollutants. After the ICPB technology was proposed, it has aroused extensive resear...
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