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Indirect ELISA antibody identification detection kit for African swine fever virus

A technology for the identification and detection of African swine fever virus, which is applied in the field of virus detection, can solve the problems of inability to realize the identification of African swine fever infection antibody and immune antibody, poor sensitivity and specificity, inconvenient high-throughput detection, etc., and achieve high sensitivity High, good stability, good sensitivity and specificity effect

Inactive Publication Date: 2021-05-25
禾旭(郑州)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to aim at the simple structure of the existing test kit or detection card for detection of African swine fever infection antibody, which cannot realize the identification of African swine fever infection antibody and immune antibody, poor sensitivity and specificity, and is not convenient to realize high-pass Quantitative detection, detection efficiency and detection speed can not meet the defects and shortcomings of the requirements, provide a reasonable structure, easy to use, can realize the identification of African swine fever infection antibody and immune antibody, good sensitivity and specificity, easy to realize high-pass An indirect ELISA antibody identification kit for African swine fever virus with greatly improved detection efficiency and detection speed

Method used

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  • Indirect ELISA antibody identification detection kit for African swine fever virus
  • Indirect ELISA antibody identification detection kit for African swine fever virus
  • Indirect ELISA antibody identification detection kit for African swine fever virus

Examples

Experimental program
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Effect test

preparation example Construction

[0020] The preparation method of the color developing solution A is as follows: weigh 5.76g of citric acid and add appropriate amount of pure water, fully mix, adjust the pH value to about 3.0 with hydrochloric acid, add 0.5g of urea peroxide and 6.21g of sodium acetate to make it completely Dissolve, add Proclin-3000.5mL, then add double distilled water to make up to 1000mL, store at 2~8℃.

[0021] The preparation method of the chromogenic solution B is as follows: Weigh 0.2g of TMB and add it into pure water, adjust the pH to about 3.0 with hydrochloric acid, add 5.76g of citric acid, and dissolve it completely, then add 0.5mL of Proclin-300, and use a graduated cylinder to Measure double-distilled water to make up to 1000mL, and store at 2-8°C.

[0022] The nucleotide sequence of the P54 protein is shown in the sequence table SEQ ID No.1, and the nucleotide sequence of the CD2v protein is shown in the sequence table SEQ ID No.2,

[0023] The detection kit of the present in...

example 2

[0050] Example 2, the main index of the identification ELISA antibody detection method of African swine fever virus

[0051] sensitivity

[0052] The positive quality control serum was carried out at 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128, 1:256, 1:512, 1:1024, 1:2048 Dilution, adopt the differential ELISA antibody detection kit of African swine fever virus to detect the serum after the above dilution, the data are shown in the following table:

[0053] Table 1 Sensitivity test of African swine fever virus identification ELISA antibody detection method

[0054]

[0055] The test results show that the data of the three repeated experiments are consistent, the minimum detection amount of P54 can reach 1:128, and the minimum detection amount of CD2v can reach 1:256.

[0056] specificity

[0057] The 2 specific quality control sera were tested with 3 batches of African swine fever differential ELISA antibody detection kits to verify the specificity of the product.

[0058] ...

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Abstract

The invention relates to an indirect ELISA antibody identification and detection kit for the African swine fever virus. The kit comprises a coating plate, a sample diluent, an enzyme conjugate, a positive control, a negative control, a color developing solution A, a color developing solution B, a concentrated washing solution, a solid washing solution, a stop solution, a sample adding groove for sample adding, a plate sealing film and a serum diluting plate for serum dilution. According to the kit of the invention, the indirect ELISA technology is applied; on the basis of an indirect method principle, through detecting an African swine fever virus antibody, an African swine fever virus wild virus infection antibody and a vaccination generated antibody are further identified and detected, so that the African swine fever virus wild virus infection antibody and the vaccine immune antibody are distinguished; and the sensitivity and the specificity of the kit are good, high-throughput detection is convenient to realize, and the detection efficiency and the detection speed are greatly improved.

Description

technical field [0001] The invention relates to a virus detection kit, in particular to an African swine fever virus indirect ELISA antibody identification detection kit, which belongs to the technical field of virus detection. Background technique [0002] African swine fever (African swine fever, ASF) is an acute, highly contagious disease of domestic pigs and wild boars caused by African swine fever virus (ASFV). Organ hemorrhage is the main feature, and the general death is 2-10 days after the onset, and the mortality rate can reach 100% in severe cases. The disease belongs to the Class A animal disease that must be reported by the World Organization for Animal Health (OIE), and it is listed as a Class I animal disease in my country. African swine fever is endemic in dozens of countries in Africa, Europe and the Americas, causing huge economic losses and has spread to many Asian countries bordering Europe. [0003] The African swine fever epidemic has broken out in lar...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/543
CPCG01N33/543G01N33/56983G01N2333/01
Inventor 鲁龙步志高赵东明刘近李芳郭宇谢彩华江涛任灵芝杨转姬杰菲梁静石书霞陈焕姣
Owner 禾旭(郑州)生物技术有限公司
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