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Artificial feed for promoting rapid growth and reproduction of springtails and preparation method thereof

A technology for artificial feed and springtails, which is applied in the field of microorganisms, can solve the problems that the number of pests obtained is small, the number of tests cannot be reached, and the rapid growth and reproduction of springtails cannot be satisfied.

Inactive Publication Date: 2021-05-28
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The first purpose of the present invention is to provide a kind of artificial feed that promotes the rapid growth and reproduction of Collembola, to overcome the problem that although the existing formula of insect artificial feed can make Collembola reproduce, the number of the pests obtained is still small, which is far from reaching The number of experiments simply cannot meet the requirements for rapid growth and reproduction of Collembola

Method used

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  • Artificial feed for promoting rapid growth and reproduction of springtails and preparation method thereof
  • Artificial feed for promoting rapid growth and reproduction of springtails and preparation method thereof
  • Artificial feed for promoting rapid growth and reproduction of springtails and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] This example illustrates the preparation of the feed.

[0023] Step 1: Take by weighing dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate heptahydrate (MgSO 4 ·7H 2 (2) 0.5g, ferrous sulfate is 0.01g, tryptophan 3g, soybean powder 200g, vitamin C 3g, sucrose 30g, agar is 20g;

[0024] Step 2: Mix the weighed medicines together, add deionized water to make the volume to 1000mL;

[0025] Step 3: Place the prepared feed in an autoclave, autoclave at 121°C for 30 minutes, and then take it out for use;

[0026] Step 4: Put the sterilized solid feed into a grinder and crush it to a size of 8-15mm×8-15mm×8-15mm, mix it with rice bran at a weight ratio of 1:1, and place it in a culture tank.

Embodiment 2

[0028] This example illustrates the preparation of the feed.

[0029] Step 1: Take by weighing dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate heptahydrate (MgSO 4 ·7H 2 (2) 0.5g, ferrous sulfate is 0.01g, lysine 3g, clover powder 200g, vitamin C 1.5g, vitamin E1.5g, sucrose 30g, agar is 20g;

[0030] Step 2: Mix the weighed medicines together, add deionized water to make the volume to 1000mL;

[0031] Step 3: Place the prepared feed in an autoclave, autoclave at 121°C for 30 minutes, and then take it out for use;

[0032] Step 4: Put the sterilized solid feed into a grinder and crush it to a size of 8-15mm×8-15mm×8-15mm, mix it with rice bran at a weight ratio of 1:1, and place it in a culture tank.

Embodiment 3

[0034] This example illustrates the preparation of the feed.

[0035] Step 1: Take by weighing dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate heptahydrate (MgSO 4 ·7H 2 (2) 0.5g, ferrous sulfate is 0.01g, methionine 3g, soybean powder 200g, vitamin E 3g, sucrose 30g, agar is 20g;

[0036] Step 2: Mix the weighed medicines together, add deionized water to make the volume to 1000mL;

[0037] Step 3: Place the prepared feed in an autoclave, autoclave at 121°C for 30 minutes, and then take it out for use;

[0038] Step 4: Put the sterilized solid feed into a grinder and crush it to a size of 8-15mm×8-15mm×8-15mm, mix it with rice bran at a weight ratio of 1:1, and place it in a culture tank.

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Abstract

The invention relates to an artificial feed for promoting rapid growth and reproduction of springtails. The artificial feed comprises the following components: 2-5g / L of essential amino acid, 200g / L of protein, 2-5g / L of vitamin, 1-4g / L of dipotassium phosphate, 0.1-2g / L of magnesium sulfate heptahydrate, 0.1-2g / L of potassium chloride, 0.01-1g / L of ferrous sulfate, 15-25g / L of agar and the balance of water. The essential amino acid is any one of lysine, tryptophan or methionine, the protein is radix tetrastigme powder or bean flour, and the vitamin is vitamin C and / or vitamin E. The invention also relates to a preparation method and a use method of the feed. According to the feed disclosed by the invention, the growth speed and biomass of the springtails are greatly increased, the springtails can breed one generation after being cultured for 7 days, the number of the springtails can reach more than 600, and the requirement on the quantity of the springtails in the actual research work of biology and entomology can be fully met.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an artificial feed for promoting rapid growth and reproduction of collembola and a preparation method thereof. Background technique [0002] Purple springtail (Hypogastrura communis) belongs to Collembola (Collembola) Hypogastrura family (Hypogastrura), purple springtail is the most common species of damage to mushroom beds, often floating in pieces on the water surface called black purple, mainly harmful to oyster mushrooms and straw mushrooms and edible fungi such as shiitake mushrooms, and sometimes endanger protected vegetables such as ice vegetables. Purple collembola often reproduces and survives on fertile and moist soil, weeds, library roads, and livestock manure, and can be seen all year round. [0003] At present, there are many types of insect artificial feeds, and different insect species have different feed formulas, such as peach borer, corn borer, Spodopt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/90A23K20/142A23K20/174A23K20/26A23K20/20A23K20/163A23K10/30A23K10/37
CPCA23K50/90A23K20/142A23K20/174A23K20/26A23K20/30A23K20/163A23K10/30A23K10/37Y02P60/87A23K20/147A23K20/22A23K50/00A23V2002/00A23K20/24A23V2250/065A23V2250/708A23V2250/628A23V2250/5024A23V2250/063A23V2250/712A23V2250/0632
Inventor 王汉荣谢昀烨方丽武军王连平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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