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Preparation method of co-reactant-free electrochemical luminescence immunosensor

An immunosensor, luminescent immunological technology, applied in the field of analysis

Active Publication Date: 2021-05-28
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no application technology that only relies on anodic oxygen or cathodic reduction to achieve co-reactant-free electrochemiluminescence

Method used

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  • Preparation method of co-reactant-free electrochemical luminescence immunosensor
  • Preparation method of co-reactant-free electrochemical luminescence immunosensor
  • Preparation method of co-reactant-free electrochemical luminescence immunosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] The preparation of oil-soluble indium phosphide nanocrystals (InP NCs): put 0.34mmol indium bromide, 2.2mmol zinc bromide and 5mL3mmol / L oleylamine into a three-necked flask, mix them in a temperature-controlled heating magnetic stirrer, and pass into Nitrogen, stirred at 120°C for 1h, removed water and oxygen in the mixed solution until the solution was clear; continued to heat up to 200°C, added 1mL tris(dimethylamino)phosphine and 1mL oleylamine with a concentration of 2.4mmol / mL, and reacted for 20min Stop heating until the solution turns from colorless to red, and obtain an oil-soluble indium phosphide nanocrystal dispersion solution; then centrifuge the obtained dispersion solution, and wash the obtained precipitate with ethanol to obtain oil-soluble indium phosphide nanocrystals.

[0079] The oil-soluble indium phosphide nanocrystals are tested by fluorescence ultraviolet spectrum, and the fluorescence ultraviolet spectrum is shown as figure 1 shown.

[0080] Pr...

Embodiment 2

[0094] The steps are the same as in Example 1, except that the prepared indium phosphide / zinc sulfide nanocrystal solution is dropped on a copper grid to observe the morphology. Test the high-power transmission electron micrographs of the prepared water-soluble indium phosphide / zinc sulfide nanocrystals, such as Figure 5 shown.

Embodiment 3

[0096] The steps are the same as in Example 1, except that the prepared indium phosphide / zinc sulfide nanocrystal solution is drip-dried and dried to make the X-ray diffraction spectrum of the prepared water-soluble indium phosphide / zinc sulfide nanocrystal for powder testing, Such as Figure 6 shown.

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Abstract

The invention belongs to the field of analytical technical methods, and relates to a preparation method of a co-reactant-free electrochemical luminescence immunosensor, wherein the preparation method mainly comprises the steps: (1) preparing an oil-soluble indium phosphide nanocrystalline dispersion solution by using indium bromide, tris(dimethylamino) phosphine, zinc bromide and oleylamine as raw materials; (2) preparing oil-soluble indium phosphide / zinc sulfide nanocrystals by taking the oil-soluble indium phosphide nanocrystals, zinc acetate and n-dodecanethiol as raw materials; (3) dissolving the oil-soluble indium phosphide / zinc sulfide nanocrystals in dichloromethane, and adding N,N-dimethylformamide, thiohydracrylic acid and thiomalic acid to prepare a water-soluble indium phosphide / zinc sulfide nanocrystal solution; (4) preparing a water-soluble indium phosphide / zinc sulfide nanocrystal labeled secondary antibody; and (5) preparing the co-reactant-free electrochemical luminescence immunosensor. In the preparation process, no co-reaction agent needs to be added, so that the damage to biological substances and cells caused by the co-reaction agent is avoided.

Description

technical field [0001] The invention belongs to the field of analytical techniques and methods, and relates to a preparation method of an electrochemiluminescence immunosensor without co-reactants, which uses indium phosphide / zinc sulfide nanomaterials as markers. Background technique [0002] Electrochemiluminescence (ECL) is a technique that combines both chemiluminescence and electrochemiluminescence. Due to its fast response, high sensitivity, low cost, and easy control, it has attracted widespread attention in bioanalysis, environmental monitoring, and clinical diagnosis. In ECL detection, high-sensitivity detection of targets depends not only on the high luminous efficiency of luminophores, but also on effective signal switching modes. [0003] Co-reactant electrochemiluminescence can rely on unidirectional anode or unidirectional cathode electrochemical drive to achieve ECL, and has high radiation efficiency and high intensity. It is currently the most common applicat...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N21/76
CPCG01N27/26G01N27/30G01N27/3278G01N21/76
Inventor 邹桂征傅莉
Owner SHANDONG UNIV
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