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Method for separating flavone, saponin and polysaccharide from cyclocarya paliurus by continuous method

A technology of Cyclocarya paliurus and continuous method, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, plant/algae/fungus/moss components, etc. It can solve the problems of low yield of polysaccharides and waste of resources, and achieve high content , low production cost and high yield

Pending Publication Date: 2021-06-01
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method utilizes enzymatic hydrolysis, but does not effectively utilize flavonoids and saponins, resulting in a waste of resources; on the other hand, the yield of polysaccharides is low due to the dissolution and refining of alkaline water, decolorization with high-temperature hydrogen peroxide, and repeated alcohol precipitation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] (1) Enzymatic hydrolysis: take 20kg of dry leaf raw materials of P. chinensis, pulverize to a particle size of 1~2mm, add 20kg of water (containing 0.02kg of compound enzyme, 0.01kg of surfactant; wherein the compound enzyme is cellulase: hemifiber Pectinase: pectinase mass ratio = 2:2:1, the surfactant is dodecyl ethoxy sulfobetaine) infiltration, mixed evenly, and enzymatically hydrolyzed at room temperature for 6 hours;

[0055] (2) Percolation extraction: the raw materials after enzymatic hydrolysis are put into the percolator, compressed, and 200L of ethanol solution with a volume fraction of 97% is added, and percolation is extracted at room temperature. The percolation flow rate is 0.5BV / hour to obtain percolation liquid , the seepage slag is reserved for use;

[0056] (3) Filtration: the leachate is filtered with a ceramic membrane having a pore size of 50 nm (the filtration pressure is 0.03 Mpa) to obtain an ethanol filtrate;

[0057] (4) Decolorization: the e...

Embodiment 2

[0062] (1) Enzymolysis: take 20kg of dry leaf raw materials of P. chinensis, pulverize to a particle size of 1~2mm, add 18kg of water (containing 0.015kg of compound enzyme, 0.01kg of surfactant; wherein the compound enzyme is cellulase: hemifiber Pectinase: pectinase mass ratio = 4:4:1.5, the surfactant is benzalkonium chloride) infiltration, mixed evenly, and enzymatically hydrolyzed at room temperature for 8 hours;

[0063] (2) Percolation extraction: the raw materials after enzymolysis are put into the percolator, compressed, and 240L of ethanol solution with a volume fraction of 95% is added, and percolation is extracted at room temperature. The percolation flow rate is 0.4BV / hour, and the percolation solution is obtained , the seepage slag is reserved for use;

[0064] (3) Filtration: leachate is filtered with a ceramic membrane with a pore size of 50nm (the filtration pressure is 0.05Mpa) to obtain ethanol filtrate;

[0065] (4) decolorization: the ethanol filtrate is ...

Embodiment 3

[0070] (1) Enzymatic hydrolysis: take 20kg of dry leaf raw materials of P. chinensis, pulverize to a particle size of 1~2mm, add 20kg of water (containing 0.02kg of compound enzyme, 0.01kg of surfactant; wherein the compound enzyme is cellulase: hemifiber Pectinase: pectinase mass ratio = 4:2:1, the surfactant is sodium lauryl sulfate) infiltration, mixed evenly, and enzymatically hydrolyzed at room temperature for 6 hours;

[0071] (2) Percolation extraction: the raw materials after enzymolysis are put into the percolator, compressed, and 300L of ethanol solution with a volume fraction of 98% is added, and percolation is extracted at room temperature. The percolation flow rate is 0.5BV / hour, and the percolation solution is obtained , the seepage slag is reserved for use;

[0072] (3) Filtration: the leachate is filtered with a ceramic membrane having a pore size of 50 nm (the filtration pressure is 0.03 Mpa) to obtain an ethanol filtrate;

[0073] (4) decolorization: the eth...

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Abstract

The invention relates to a method for separating flavone, saponin and polysaccharide from cyclocarya paliurus by a continuous method. The method comprises the following steps: enzymolysis, percolation extraction, filtration, decoloration, polyamide adsorption separation of flavone and saponin, and separation and purification of polysaccharide. The enzymolysis is performed under the action of a compound enzyme and an ionic surfactant. The invention provides a brand new method for separating various natural active components from cyclocarya paliurus, the process has strong operability, no acid or alkali is used, toxic, harmful, flammable and explosive chemical solvents are not used, the method is safe, environment-friendly, pollution-free and low in production cost, efficient and comprehensive utilization of cyclocarya paliurus resources is also realized, and the method is suitable for industrial production. The three obtained cyclocarya paliurus active ingredient products are high in yield and content, and the method is a process for remarkably improving the economic value of a cyclocarya paliurus extraction process.

Description

technical field [0001] The invention relates to a method for separating natural active components of Radix vulgaris, in particular to a continuous method for separating flavonoids, saponins and polysaccharides from Radix vulgaris. Background technique [0002] Cyclocarya paliurus Iljinskaja (Cyclocarya paliurus Iljinskaja) belongs to the genus Cyclocarya Iljinsk. of the dicotyledon family Juglandaceae. Widely distributed in Jiangxi, Zhejiang, Jiangsu, Anhui, Fujian, Hunan, Hubei, Sichuan, Guizhou, Yunnan and other places. According to the Records of Chinese Medicine Resources, its leaves, bark, and roots can be used as medicine, warm in nature, slightly bitter in taste, and have the functions of clearing away heat and swelling, detoxifying and relieving pain. For a long time, folks have traditionally used its leaves to make tea as a beverage, which has the effects of clearing away heat and reducing heat, lowering blood pressure, treating hypertension and diabetes, and prolo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/52C08B37/00A61K127/00
CPCA61K36/52A61K2236/19A61K2236/333A61K2236/53A61K2236/55C08B37/0003
Inventor 李伟黄华学
Owner HUNAN HUACHENG BIOTECH
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