Unlock instant, AI-driven research and patent intelligence for your innovation.

Bacterial strain for antagonizing sclerotiniose of asarum and application of bacterial strain

A kind of Sclerotinia asarum, antagonistic technology, applied in the direction of application, fungicides, fungi, etc., can solve the problems of polluting the environment, using only chemical pesticides, hindering the sustainable use of the environment, etc., and achieve fast growth and sporulation big effect

Active Publication Date: 2021-06-01
JILIN AGRICULTURAL UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to discover biocontrol bacteria and verify its control effect, overcome the shortcomings of only using chemical pesticides, polluting the environment and hindering the sustainable use of the environment in the existing technology of preventing and treating Sclerotinia, and provide a new type of antagonistic bacteria Sclerotinia pungent bacterial strains and applications thereof, the Trichoderma bacterial strains provided by the present invention have advantages such as high antagonisticity and strong specialization, and the biological bacterial agents produced by using the bacterial strains can effectively prevent and control soil-borne diseases

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacterial strain for antagonizing sclerotiniose of asarum and application of bacterial strain
  • Bacterial strain for antagonizing sclerotiniose of asarum and application of bacterial strain
  • Bacterial strain for antagonizing sclerotiniose of asarum and application of bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A strain of the present invention that antagonizes Sclerotinia sclerotiorum, the name of the strain is Trichoderma koningiopsis (Trichodermakoningiopsis) JLAUWZQ-2, and its preservation number in the General Microorganism Center of China Microbiological Culture Collection Management Committee is CGMCC No.21465 , the address of the preservation unit is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation date is January 29, 2021.

[0030] Such as figure 1 and figure 2 As shown, the Trichoderma koningiopsis (Trichoderma koningiopsis) JLAUWZQ-2 shows the following morphological characteristics: on the potato dextrose agar medium, it is lawn-like, and the conidia are evenly distributed on the aerial hyphae and are granular. Green, with white aerial hyphae on the edge, conidia with a diameter of 0.5mm-2mm, conidiophores with an obvious main axis, the width is 2.8μm-3.5μm, and...

Embodiment 2

[0054] A kind of inhibition of Trichoderma pseudoconii JLAUWZQ-2 bacterial strain n-butanol extract (mixture) of the present invention to the inhibition of Sclerotinia asarum comprises the following steps: 50mL Trichoderma pseudoconii JLAUWZQ-2 bacterial strain fermented liquid is used Extract 3 times with 50 mL of ethyl acetate, separate the ethyl acetate phase and the water phase, separate the water phase and extract 3 times with an equal volume of water-saturated n-butanol, and evaporate the separated n-butanol phase to dryness on a rotary evaporator at 55°C , dissolved in 10 mL of methanol. Take 300 μL of methanol solution in the culture medium, and carry out the antibacterial experiment, after determination, the antibacterial rate is 100%. Such as Figure 7 As shown, after the present embodiment utilizes water-water saturated n-butanol-ethyl acetate to separate and extract the metabolites of the JLAUWZQ-2 bacterial strain, its n-butanol phase mixture has an inhibitory ra...

Embodiment 3

[0056] A kind of Trichoderma pseudoconii JLAUWZQ-2 bacterial strain n-butanol extract (monomer) of the present invention is to the inhibition of Sclerotinia asarum, the early stage process is the same as embodiment 2, after obtaining n-butanol extract, to n-butanol After the alcohol extract was analyzed by LC-MS, it was found that there were many antibacterial active ingredients in JLAUWZQ-2, such as Figure 8 As indicated, the concentration of the monomeric compound avermectin or methyl eugenol in its composition is greater than or equal to 300 μg·mL -1 When the antibacterial rate reaches 100%, the concentration of monomeric compounds such as docosanoic acid, minocycline or erythromycin is greater than or equal to 500 μg·mL -1 When the antibacterial rate reaches 100%, the antibacterial effect is remarkable.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Widthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a bacterial strain for antagonizing sclerotiniose of asarum and application of the bacterial strain, and belongs to the technical field of plant protection. The strain is Trichoderma koningiopsis JLAUWZQ-2, and the preservation number of the Trichoderma koningiopsis JLAUWZQ-2 is CGMCC No.21465 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. The strain has the advantages of high antagonism, strong specificity and the like, is mainly applied to antagonism of asarum sclerotiniose, including root irrigation with bacterial liquid, seed soaking with bacterial liquid, seedling spraying with bacterial liquid and the like, can effectively inhibit the growth of asarum sclerotiniose bacteria, and has the advantages of high control effect, good environmental safety and good development and application prospects.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and relates to a bacterial strain antagonizing Sclerotinia asarum and its application, in particular to a Trichoderma pseudoconii JLAUWZQ-2 for efficiently preventing and treating Sclerotinia asarum and its application. Background technique [0002] Asarum heterotropoides Fr. Schmidt Var. mandshuricum (Maxim) kitag. is a large amount of traditional Chinese medicine in Northeast China. It is the first authentic product included in the Pharmacopoeia of the People's Republic of China. Headache, phlegm, cough and asthma, joint pain, nasal congestion, toothache, etc.; in the chemical industry, it is often used in the product development of targeted drugs, daily necessities, flavors, insecticides, and green pesticides. In addition to medicinal parts, asarum leaves contain a large amount of phenylpropanoid compounds such as cinnamic acid and coumaric acid, which have anti-oxidation, antibacteri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14A01N63/38A01P3/00C12R1/885
CPCA01N63/38
Inventor 王志清王子晴卢宝慧马海琴张敏权兴周赵光远
Owner JILIN AGRICULTURAL UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com