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Decellularized small intestinal submucosa/polylactic acid-glycolic acid copolymer composite scaffold as well as preparation method and application thereof

A small intestinal submucosa and glycolic acid technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve problems such as unsatisfactory repair effect and suboptimal overall biological activity, achieve good tissue repair function, and promote mucosal repair. Effect of cell migration and growth, high biological activity

Pending Publication Date: 2021-06-04
NINGBO MEDICAL CENT LIHUILI HOSPITACL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, in order to meet the requirements of strength and other mechanical properties, the whole or most of the stent is made of synthetic polymer materials, resulting in the suboptimal overall biological activity; Microstructure that is conducive to promoting tissue repair, resulting in unsatisfactory repair effects

Method used

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  • Decellularized small intestinal submucosa/polylactic acid-glycolic acid copolymer composite scaffold as well as preparation method and application thereof
  • Decellularized small intestinal submucosa/polylactic acid-glycolic acid copolymer composite scaffold as well as preparation method and application thereof
  • Decellularized small intestinal submucosa/polylactic acid-glycolic acid copolymer composite scaffold as well as preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0032] The invention provides a method for preparing a decellularized small intestinal submucosa / polylactic acid-glycolic acid copolymer composite scaffold. The composite scaffold preparation method comprises: electrospinning a spinning solution containing polylactic acid-glycolic acid copolymer (PLGA) into On the decellularized small intestinal submucosa (dSIS), a decellularized small intestinal submucosa / polylactic acid-glycolic acid copolymer composite scaffold was prepared.

[0033] Poly(lactic-co-glycolic acid) was electrospun onto the decellularized small intestinal submucosa attached to the receiver by electrospinning to prepare a composite scaffold with a layered structure. The decellularized intestinal submucosa in the composite scaffold supported the growth of muscle layer cells, and the poly(lactic-co-glycolic acid) layer supported the growth of mucosal layer cells.

[0034] PLGA in the prior art is mainly used to mechanically enhance dSIS rather than promote epithe...

Embodiment 1

[0057] This example provides a method for preparing a decellularized small intestinal submucosa / polylactic acid-glycolic acid copolymer composite scaffold, which includes the preparation of decellularized small intestinal submucosa (dSIS) and the preparation of dSIS / PLGA composite scaffold.

[0058] Specifically, the preparation of decellularized small intestinal submucosa (dSIS) includes the following steps:

[0059] Small intestines were harvested from healthy pigs (about 6 months of age and about 100 kg body weight) within 4 hours of slaughter. The serosa, muscular layer, and mucosa were mechanically removed, and the remaining submucosa was rinsed with deionized water. The decellularization process included: (1) treatment of small intestinal submucosa (SIS) with acetone and ethanol (2:1, v / v), (2) treatment with 0.05% trypsin and 0.02% EDTA at 37°C for 8 h, (3 ) treated with 4% SDS and 3% TritonX-100 for 24h, (4) treated with 0.1% peracetic acid and 20% ethanol for 30min. ...

Embodiment 2

[0065] This example provides a method for preparing a decellularized small intestinal submucosa / polylactic acid-glycolic acid copolymer composite scaffold, which includes the preparation of decellularized small intestinal submucosa (dSIS) and the preparation of dSIS / PLGA composite scaffold.

[0066] Compared with Example 1, the only difference lies in the different number of stacked layers of single-layer dSIS and the different electrospinning conditions. In this example, 6 pieces of single-layer dSIS prepared as in Example 1 were stacked, and the electrospinning conditions were set as follows:

[0067] The voltage is 20kV, the flow rate of the needle is 3ml / h, and the distance from the needle tip to the receiver is 15cm. Electrospinning continued for 1 h to form dSIS / PLGA composite scaffolds.

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Abstract

The invention discloses a decellularized small intestinal submucosa / polylactic acid-glycolic acid copolymer composite scaffold as well as a preparation method and application thereof, and relates to the technical field of tissue repair. The shape of the composite scaffold is similar to a layered structure of laryngeal pharynx and neck esophageal tissues. In the composite scaffold, the amount of residual DNA of the decellularized small intestinal submucosa is small, the composite scaffold has low immunogenicity and high biological activity, the polylactic acid-glycolic acid copolymer is arranged in a nanofiber mode and can promote migration and growth of mucosa cells, and the composite scaffold has good mechanical properties. Through verification, interaction between cells and each layer of the scaffold is good, ideal cell proliferation and cell phenotypes are shown, and it is proved that the composite scaffold has a good tissue repair function.

Description

technical field [0001] The invention relates to the technical field of tissue repair, in particular to a decellularized small intestinal submucosa / polylactic acid-glycolic acid copolymer composite scaffold and a preparation method and application thereof. Background technique [0002] Currently, the incidence of throat and neck esophageal cancer has increased around the world due to environmental pollution problems, excessive drinking and smoking in certain populations, and the habit of chewing betel nuts in some areas. A significant proportion of patients with such cancers require laryngopharyngectomy and / or esophagectomy, which inevitably results in tissue loss. Among them, large tissue loss is usually repaired using the patient's own muscular skin flap or intestinal segment, and these operations are destructive and may cause secondary disease in the patient, so surgeons and researchers have been actively looking for suitable tissue repair materials to solve the above pro...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/36A61L27/18A61L27/50A61L27/56
CPCA61L27/3629A61L27/18A61L27/3679A61L27/3687A61L27/3691A61L27/56A61L27/50A61L2430/22C08L67/04
Inventor 裘世杰邹鹏梁丽金谭睿哲陈琦
Owner NINGBO MEDICAL CENT LIHUILI HOSPITACL
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