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Method for catalytically synthesizing vitamin A palmitate by immobilized enzyme

A technology of palmitate and immobilized enzymes, which is applied in the field of medicine, can solve the problems of short service life of enzyme catalysts, low conversion rate, difficult regeneration, etc., and achieve the effects of many application times, high product conversion rate and low cost

Pending Publication Date: 2021-06-08
ZHEJIANG MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Compared with the current domestic traditional chemical synthesis process, the bio-enzyme catalyzed production of vitamin A palmitate currently uses the transesterification method, but the conversion rate is not high, resulting in the coexistence of vitamin A palmitate and vitamin A acetate. Subsequent separation will be very difficult, and there are problems such as short service life of the enzyme catalyst and difficult regeneration

Method used

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  • Method for catalytically synthesizing vitamin A palmitate by immobilized enzyme
  • Method for catalytically synthesizing vitamin A palmitate by immobilized enzyme
  • Method for catalytically synthesizing vitamin A palmitate by immobilized enzyme

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1: VA alcohol preparation

[0027] Accurately weigh 50g (0.147mol) of vitamin A acetate and place it in a 500ml three-necked bottle, add 100ml of methanol to replace the air in the bottle with nitrogen, stir and mix, stir at 100-200rpm, and add sodium hydroxide lye dropwise at 10°C After 0.5 hours, the reaction was completed, extracted with 500ml n-heptane, and washed with water until neutral. The n-heptane extract was detected by HPLC method, and the conversion rate of alcoholysis was 100%.

Embodiment 2

[0028] Embodiment 2: Esterification reaction

[0029] The vitamin A alcohol solution prepared in Example 1 was placed in a 1000ml three-necked bottle, 44g (0.17mol) of palmitic acid was added, and 10g of immobilized lipase (purchased from Novozymes Co. TL IM lipase) The purchased enzyme is silica gel immobilized enzyme, add 600ml n-hexane, replace the air with nitrogen, reflux reaction at 10°C, and remove the water generated in the reaction process, react for 3 hours. The esterification solution was taken and detected by HPLC method, and the conversion rate of esterification was 99.6%.

Embodiment 3

[0031] The operation steps are the same as in Example 2, the difference is that different types of dehydrating agents such as anhydrous sodium sulfate, anhydrous magnesium sulfate, 4A molecular sieve, etc. are added in the normal pressure esterification process, and the effectiveness of different dehydration methods is investigated. The results are shown in Table 1 .

[0032] Table 1

[0033]

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PUM

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Abstract

The invention relates to a preparation method for catalytically synthesizing vitamin A palmitate by immobilized enzyme. The method comprises the following steps: adding palmitic acid, immobilized lipase and a non-polar solvent into a vitamin A alcohol solution in an inert environment to perform esterification reaction, removing water generated by esterification so as to separate the water generated by esterification reaction from an organic solvent, to obtain esterified liquid; desolventizing, crystallizing and filtering the esterified liquid to obtain the high-purity vitamin A palmitate. The method has advantages of high esterification conversion rate (greater than or equal to 99%), small use amount of the immobilized enzyme, simple application mode, long service life, capability of meeting industrial production conditions, good product quality, and conformity to CP\EP\USP and other existing pharmacopeia quality standard requirements.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to a method for preparing vitamin A palmitate. The specific content is that vitamin A alcohol and palmitic acid are catalyzed by immobilized enzymes and dehydrated to generate vitamin A palmitate. The product obtained by the invention has high yield and high purity. Background technique [0002] Vitamin A, also known as retinol, is one of the essential nutrients for the human body. It is an indispensable micronutrient during the growth and development of children. It participates in various physiological processes of the body and can maintain and promote human growth, development, reproduction and cell membranes. The stability has a significant effect on the formation of vision. Because of its anti-inflammatory, anti-oxidation, immune regulation, anti-skin aging, anti-cancer and other effects, it has been widely used in food, medicine, nutritional health products, cosmetics, and fee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12N11/14C12N11/04C12N11/08
CPCC12P23/00C12N9/20C12Y301/01003C12N11/14C12N11/04C12N11/08
Inventor 黑延琳梁智平周志祥何剑锋白亚龙王红卫
Owner ZHEJIANG MEDICINE CO LTD
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