Arthrinium fungus strain and application thereof

A technology of Arthrobium sp. and genus, which is applied in the field of microorganisms and can solve the problems such as the limitation of 3-nitropropionic acid method

Active Publication Date: 2021-06-11
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current methods for producing 3-nitropropionic acid are relatively limited

Method used

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  • Arthrinium fungus strain and application thereof
  • Arthrinium fungus strain and application thereof
  • Arthrinium fungus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Screening process:

[0019] Collect moldy sugarcane samples from different provinces and regions in the country, cut sugarcane pieces by aseptic operation, plant them on potato dextrose agar medium, and cultivate them at 28°C. For secondary purification, the purified strains were stored at 4°C on a PDA slant. Observe the colony morphology, and observe characteristics such as spore morphology and size under a microscope. The strain was initially identified as Arthrinium, and then further verified by ITS sequence comparison analysis, and it was confirmed that the strain isolated from sugarcane was Arthrinium arundinis.

[0020] 2. Colony morphology:

[0021] PDA colonies are off-white, flat, dense cotton-wool-like, and the matrix hyphae are yellowish (such as figure 1 Shown), the hyphae consist of smooth, transparent, branched, spaced hyphae with a diameter of 2-3 μm. Conidiophores erect, septated, light brown, smooth, degenerated into meristematic cells. Meriste...

Embodiment 2

[0030] 1. Detection of 3-nitropropionic acid

[0031] HPLC conditions

[0032] 1. Detector: PDA detector

[0033] 2. Detection wavelength: 210nm (3D scanning range: 190~290nm)

[0034] 3. Mobile phase: acetonitrile+20mmol / L potassium dihydrogen phosphate (adjust pH=3.0)=20+80

[0035] 4. Chromatographic column: Thermo syncronis C18 5μ (250×4.6mm)

[0036] 5. Column temperature: 35°C

[0037] 6. Injection volume: 10μL

[0038] 3. Sample Processing

[0039] Select the Arthrinium arundinis T5-2 strain that was purified many times in the screening process of Example 1, inoculate it on a PDA medium plate, cultivate it at 28° C. for 7 days, and punch 3 mycelium cakes with a 5 mm puncher to insert PYS liquid medium (potato-yeast extract-sucrose), 28°C, 130r / min shaking culture for 14d. Take 5 mL of the PYS culture solution filtered by four layers of gauze into a 50 mL centrifuge tube, add 15 mL of acetonitrile and vortex mix for 1 min, add 3 g of sodium chloride after ultrason...

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Abstract

The invention discloses an arthrinium fungus strain. The arthrinium fungus strain is named as Arthrinium arundinis T5-2 and is preserved in the Guangdong Microbial Culture Collection Center on October 23, 2020, the preservation address is the 5th floor, No.59 building, No.100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No.61237. According to the invention, the Arthrinum arundinis T5-2 is separated and screened from sugarcane for the first time, the Arthrinum arundinis T5-2 can secrete 3-nitropropionic acid, the yield is high, and a wide application prospect is brought to the field of production of 3-nitropropionic acid; and furthermore, the Arthrinium arundinis T5-2 disclosed by the invention can also be used for secreting alcohol chemical substances such as ethanol.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a fungus strain of the genus Arthrospora and its application. Background technique [0002] Microorganisms are the main source of natural products in nature. 3-Nitropropionic acid (β-Nitropropionic acid) is a toxic metabolite produced by fungi such as Aspergillus flavus, Arthrospora, and actinomycetes. There are also 3-nitropropionic acids in some higher plants. Nitropropionic acid is present. 3-Nitropropionic acid is a very useful organic chemical intermediate, which can be used in the synthesis of β-amino acid, and can also be used in the preparation of the key intermediate of Aliskiren. However, the current methods for producing 3-nitropropionic acid are relatively limited. Contents of the invention [0003] The object of the present invention is to provide a Arthrinium arundinis strain capable of secreting 3-nitropropionic acid and producing ethanol and...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/06C12P13/00C12R1/645
CPCC12P7/06C12P13/008Y02E50/10
Inventor 廖洁何洁李慧玲莫磊兴蒋文艳吴小建王天顺程亮陈伟闫飞燕牙禹韦宇宁
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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