Arthrinium fungus strain and application thereof
A technology of Arthrobium sp. and genus, which is applied in the field of microorganisms and can solve the problems such as the limitation of 3-nitropropionic acid method
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Embodiment 1
[0018] 1. Screening process:
[0019] Collect moldy sugarcane samples from different provinces and regions in the country, cut sugarcane pieces by aseptic operation, plant them on potato dextrose agar medium, and cultivate them at 28°C. For secondary purification, the purified strains were stored at 4°C on a PDA slant. Observe the colony morphology, and observe characteristics such as spore morphology and size under a microscope. The strain was initially identified as Arthrinium, and then further verified by ITS sequence comparison analysis, and it was confirmed that the strain isolated from sugarcane was Arthrinium arundinis.
[0020] 2. Colony morphology:
[0021] PDA colonies are off-white, flat, dense cotton-wool-like, and the matrix hyphae are yellowish (such as figure 1 Shown), the hyphae consist of smooth, transparent, branched, spaced hyphae with a diameter of 2-3 μm. Conidiophores erect, septated, light brown, smooth, degenerated into meristematic cells. Meriste...
Embodiment 2
[0030] 1. Detection of 3-nitropropionic acid
[0031] HPLC conditions
[0032] 1. Detector: PDA detector
[0033] 2. Detection wavelength: 210nm (3D scanning range: 190~290nm)
[0034] 3. Mobile phase: acetonitrile+20mmol / L potassium dihydrogen phosphate (adjust pH=3.0)=20+80
[0035] 4. Chromatographic column: Thermo syncronis C18 5μ (250×4.6mm)
[0036] 5. Column temperature: 35°C
[0037] 6. Injection volume: 10μL
[0038] 3. Sample Processing
[0039] Select the Arthrinium arundinis T5-2 strain that was purified many times in the screening process of Example 1, inoculate it on a PDA medium plate, cultivate it at 28° C. for 7 days, and punch 3 mycelium cakes with a 5 mm puncher to insert PYS liquid medium (potato-yeast extract-sucrose), 28°C, 130r / min shaking culture for 14d. Take 5 mL of the PYS culture solution filtered by four layers of gauze into a 50 mL centrifuge tube, add 15 mL of acetonitrile and vortex mix for 1 min, add 3 g of sodium chloride after ultrason...
PUM
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