Liquid culture medium and culture method for ginseng adventitious root culture

Abstract

The invention discloses a liquid culture medium and a culture method for ginseng adventitious root culture. The liquid culture medium is prepared from the components: 10-55 g / L of cane sugar, 0.6-1.6 g / L of a B5 culture medium, 0.3-1.2 g / L of a 1 / 2 MS culture medium, 0-5.4 mg / L of 6-benzylamino adenine, 0-5.4 mg / L of naphthylacetic acid, 0-8 mg / L of gibberellin and 0-5.4 mg / L of indoleacetic acid; the culture method comprises the following steps of cleaning, disinfecting and slicing mature ginseng, inoculating into an induction culture medium to induce ginseng adventitious roots, inoculating the obtained ginseng adventitious roots into the induction culture medium again for subculture and propagation, cutting the obtained ginseng adventitious roots into segments, inoculating the segments into the liquid culture medium, and culturing the segments to obtain the adventitious roots. By adopting the liquid culture medium and the culture method disclosed by the invention, each part of the mature ginseng can be directly induced to generate the adventitious roots, and an intermediate step of inducing calluses is not needed, so that the induction step can be simplified, the induction time can be shortened, and meanwhile, the content of ginsenoside in the adventitious roots can be greatly increased.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a liquid culture medium and a culture method for culturing adventitious roots of ginseng. Background technique [0002] Ginseng (Panax ginseng C.A.Mey.) is a plant of the genus Panax of Araliaceae, distributed in China, Japan, and Korea. Its rhizome is a valuable Chinese medicinal material, known as the "King of Herbs". Ginseng is sweet, slightly bitter, and slightly warm. It has the functions of invigorating vital energy, restoring pulse and strengthening the spleen, nourishing the spleen and lung, promoting body fluid and nourishing blood, calming the nerves and improving intelligence, etc. Wound thirst, palpitations, insomnia, etc. Ginsenoside is the main active ingredient of ginseng, which has the effects of anti-fatigue, anti-aging, regulating the central nervous system, improving immunity, improving cardiovascular and cerebrovascular insufficiency,...

AI Technical Summary

Problems solved by technology

In the above scheme, the tissue-cultured seedlings aged 28-32 days are cut into small pieces to directly induce adventitious roots. The tissue-cultured seedlings are tender and have strong differentiation ability, and actually need to undergo seed germination or explant culture to obtain tissue-cultured plants. For seedlings, it still takes at least 28-32 days, explant culture still needs to induce callus, and the cycle is not actually shortened

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